Research papers

@article{Nakarada2024880,

title = {Bridging the buzz: In vivo EPR imaging unlocking the secrets of honey bee health},

author = {Đura Nakarada and Uroš Glavinić and Marko Ristanić and Milan Popović and Jevrosima Stevanović and Zoran Stanimirović and Miloš Mojović},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85196743130&doi=10.1002%2fjez.2845&partnerID=40&md5=ca2e9b540623c23a9500468f67ddf5a5},

doi = {10.1002/jez.2845},

year  = {2024},

date = {2024-01-01},

journal = {Journal of Experimental Zoology Part A: Ecological and Integrative Physiology},

volume = {341},

number = {8},

pages = {880 – 884},

abstract = {Honey bees play a pivotal role in shaping ecosystems and sustaining human health as both pollinators and producers of health-promoting products. However, honey bee colony mortality is on the rise globally, driven by various factors, including parasites, pesticides, habitat loss, poor nutrition, and climate change. This has far-reaching consequences for the environment, economy, and human welfare. While efforts to address these issues are underway, the current progress in electron paramagnetic resonance (EPR) instrumentation affords using the immense potential of this magnetic resonance technique to study small samples such as honey bees. This paper presents the pioneering 2D in vivo EPR imaging experiment on a honey bee, revealing the ongoing redox-status of bees’ intestines. This way, by monitoring the spatio-temporal changes of the redox-active spin-probes’ EPR signal, it is possible to gain access to valuable information on the course of ongoing bees’ pathologies and the prospect of following-up on the efficiency of applied therapies. Employing a selection of diverse spin-probes could further reveal pH levels and oxygen concentrations in bee tissues, allowing a noninvasive assessment of bee physiology. This approach offers promising strategies for safeguarding pollinators and understanding their biology, fostering their well-being and ecological harmony. © 2024 Wiley Periodicals LLC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jelisić2024,

title = {The Potential of Agaricus bisporus in Mitigating Pesticide-Induced Oxidative Stress in Honey Bees Infected with Nosema ceranae},

author = {Stefan Jelisić and Zoran Stanimirović and Marko Ristanić and Đura Nakarada and Miloš Mojović and Dušan Bošnjaković and Uroš Glavinić},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85210448501&doi=10.3390%2flife14111498&partnerID=40&md5=9db74c339dfae77832298776a00696a9},

doi = {10.3390/life14111498},

year  = {2024},

date = {2024-01-01},

journal = {Life},

volume = {14},

number = {11},

abstract = {Global climate change, environmental pollution, and frequent pesticide use severely reduce bee populations, greatly challenging beekeeping. Pesticides such as deltamethrin, a pyrethroid insecticide commonly used to control mosquitoes, can kill individual bees and entire colonies, depending on the exposure. Due to mosquito resistance to pyrethroid insecticides, components that enhance their effect are commonly used. This study explores the potential of Agaricus bisporus mushroom extract in mitigating oxidative stress in bees triggered by pesticides and Nosema ceranae infection. Our findings indicate that A. bisporus extract significantly reduced mortality rates of bees and spore counts of N. ceranae. Furthermore, the extract demonstrated antioxidant properties that lower enzyme activity related to oxidative stress (CAT, SOD, and GST) and MDA concentration, which is linked to lipid peroxidation. These results indicate that natural extracts like A. bisporus can aid bee health by mitigating the effects of pesticides and pathogens on honey bees, thus improving biodiversity. © 2024 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Savić2024,

title = {Glutathione Involvement in Potato Response to French Marigold Volatile Organic Compounds},

author = {Jelena Savić and Đura Nakarada and Sofija Stupar and Ljiljana Tubić and Milica Milutinović and Miloš Mojović and Nina Devrnja},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85213294791&doi=10.3390%2fantiox13121565&partnerID=40&md5=7ab0d70b1ed3fc43d4db038fa72bb955},

doi = {10.3390/antiox13121565},

year  = {2024},

date = {2024-01-01},

journal = {Antioxidants},

volume = {13},

number = {12},

abstract = {To elucidate the involvement of glutathione in the mitigation of induced oxidative changes and the sequestration of perceived volatiles in cells, we exposed potato plants to French marigold essential oil. The formation of short-lived radicals, the determination of scavenging activity towards ascorbyl and DPPH radicals, and the assessment of the potato plants’ overall intra/extracellular reduction status were performed using electron paramagnetic resonance spectroscopy (EPR). The results showed the presence of hydroxyl radicals in potatoes, with significantly reduced accumulation in exposed plants compared to the control group after 8 h. However, the kinetics of EPR signal intensity change for the pyrrolidine spin probe (3CP) in these plants showed very low reducing potential, suggesting that the antioxidant system acts lethargically and/or the probe has been reoxidized. Total glutathione and its reduced/oxidized form ratio, determined spectrophotometrically, showed that the exposed plants initially had lower glutathione levels with diminutive, reduced form compared to the control. Still, after 8 h, both characteristics were similar to those of the control. RT-qPCR analysis revealed that the volatiles altered the expression of glutathione metabolism-involved genes, especially that of glutathione-S-transferase, after 8 h. Glutathione metabolism was affected by volatiles in the initial response of potato plants exposed to French marigold essential oil, and glutathione molecules were involved in the mitigation of induced oxidative burst. © 2024 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Milutinović2023,

title = {Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening},

author = {Milica Milutinović and Đura Nakarada and Jelena Božunović and Miloš Todorović and Uroš Gašić and Suzana Živković and Marijana Skorić and Đurđa Ivković and Jelena Savić and Nina Devrnja and Neda Aničić and Tijana Banjanac and Miloš Mojović and Danijela Mišić},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149231449&doi=10.3390%2fantiox12020346&partnerID=40&md5=16739c9e61a2f5d2bfb9a909d6ad207d},

doi = {10.3390/antiox12020346},

year  = {2023},

date = {2023-01-01},

journal = {Antioxidants},

volume = {12},

number = {2},

abstract = {The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated. © 2023 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Božilović2023,

title = {Influence of 24-Epibrassinolide on the Energetic Parameters and Early Stages of Growth and Development in Seedlings of Two Maize (Zea mays L.) Genotypes},

author = {Bojana Božilović and Bogdan Nikolić and Hadi Waisi and Jelena Trifković and Vladimir Dodevski and Bojan Janković and Sanja Krstić and Miloš Mojović},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175115563&doi=10.3390%2fagronomy13071673&partnerID=40&md5=24684bb1feac960e5e5ad4c634b63533},

doi = {10.3390/agronomy13071673},

year  = {2023},

date = {2023-01-01},

journal = {Agronomy},

volume = {13},

number = {7},

abstract = {Brassinosteroids (BRs) are a class of plant hormones that play important roles in regulating various physiological and developmental processes in plants. One of the most effective BRs involved in modulating crop growth is 24-epibrassinolide (24-EBL). The effects of different concentrations of 24-EBL on various biochemical and biophysical parameters critical to early growth stages and seedling development were investigated using two maize hybrids, ‘ZP 434’ (a new-generation hybrid) and ‘ZP 704’ (an older-generation hybrid). The evaluation of results is based on measurements of germination percentage, morphometric parameters, redox status, comparative analysis of thermodynamic parameters (such as Gibbs free energy, enthalpy, entropy), and the concentration of specific sugars in different parts of maize seedlings. The results indicate that the germination and initial growth of maize seedlings are influenced by the flow of crucial sugars from the remaining seed (as a source of nutrients) towards the plumule and radicle (as sink organs). Furthermore, alterations in Gibbs free energy play a significant role in these sugar transfers within the maize seedlings. The seed germination was most affected by the highest concentrations of 24-EBL, showing inhibitory effects, whereas lower and moderate concentrations of exogenously added 24-EBL exhibited a beneficial influence on the initial phases of seedling growth. The mentioned approach gives new insights into source–sink relationships and can be used as a quantitative measure of the germination energy, which until now has been a qualitative criterion in seed science. © 2023 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Pagnacco2022,

title = {The Influence of Electrode Constituents on Hydrogen Evolution Reaction on Phosphate W- and Mo-Bronze-Based Electrodes},

author = {Maja Pagnacco and Smilja Marković and Jelena Potočnik and Vesna Krstić and Pavle Tančić and Miloš Mojović and Zorica Mojović},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140306202&doi=10.1149%2f1945-7111%2fac96ab&partnerID=40&md5=55812e236eeeb9b3f86b307056e07250},

doi = {10.1149/1945-7111/ac96ab},

year  = {2022},

date = {2022-01-01},

journal = {Journal of the Electrochemical Society},

volume = {169},

number = {10},

abstract = {Phosphate tungsten bronze (WPB) and phosphate molybdenum bronze (MoPB) were synthesized and modified with rhenium. The existing phases were established by X-ray powder diffraction (XRPD), electron paramagnetic spectroscopy (EPR) and Field emission scanning electron microscopy (FESEM). The electroactivity of bronze samples, with and without rhenium for hydrogen evolution reaction (HER) was tested. The influence of carbon black presence in the catalytic ink on the electrochemical activity was investigated. Collected results provide insight into the effects of the constituents of an electrode on its electrochemical activity. © 2022 The Electrochemical Society (“ECS”). Published on behalf of ECS by IOP Publishing Limited.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Milosavljević2022,

title = {Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars},

author = {Dragica M. Milosavljević and Vuk M. Maksimović and Jasminka M. Milivojević and Đura J. Nakarada and Miloš D. Mojović and Jelena J. Dragišić Maksimović},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144957209&doi=10.3390%2fplants11243566&partnerID=40&md5=1cbb6cf17d855eb4d9a4fe969e2fa6d6},

doi = {10.3390/plants11243566},

year  = {2022},

date = {2022-01-01},

journal = {Plants},

volume = {11},

number = {24},

abstract = {Phenolic compounds of 25 newly introduced strawberry cultivars were profiled using spectrophotometry, electron paramagnetic resonance (EPR) spectroscopy, and high-performance liquid chromatography-mass spectrometry. Total phenolic and anthocyanin content (TPC and TACY, respectively), as well as vitamin C, and concentrations of individual phenolic compounds in fruits were evaluated to identify the most promising cultivars according to their phenolic profile. The highest values of TPC, TACY, and vitamin C were recorded in ‘Premy’ (1.53 mg eq GA g−1 FW), ‘Sandra’ (30.60 mg eq Pg-3-g 100 g−1 FW), and ‘Laetitia’ (56.32 mg 100 g−1 FW), respectively. The DPPH and •OH radicals scavenging activity of fruit methanolic extracts was estimated using EPR spectroscopy. All cultivars are almost uniformly effective in the scavenging of •OH radical, while ‘Tea’, ‘Premy’, and ‘Joly’ were marked as highly potent cultivars (over 70%) in terms of DPPH-antiradical activity. Specific peroxidase activities were the highest in ‘Garda’, ‘Federica’, and ‘Rumba’ (0.11, 0.08, and 0.06 U mg−1 prot, respectively). ‘Laetitia’, ‘Joly’, ‘Arianna’, ‘Tea’, and ‘Mila’ cultivars were distinguished from others as the richest concerning almost all flavonoids and phenolic acids, including some other parameters of bioactivity. These cultivars could be recommended to consumers as functional fruit foods. © 2022 by the authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bartolić20222500,

title = {Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study},

author = {Dragana Bartolić and Miloš Mojović and Miloš Prokopijević and Daniela Djikanović and Aleksandar Kalauzi and Dragosav Mutavdžić and Rada Baošić and Ksenija Radotić},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118771838&doi=10.1002%2fjsfa.11591&partnerID=40&md5=0c702ebc1e1f64180a75b337db6ff19a},

doi = {10.1002/jsfa.11591},

year  = {2022},

date = {2022-01-01},

journal = {Journal of the Science of Food and Agriculture},

volume = {102},

number = {6},

pages = {2500 – 2505},

abstract = {BACKGROUND: Aflatoxin B1 (AFB1) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB1 and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB1-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB1. RESULTS: Different changes in the content of lignin and free radicals with increasing AFB1 concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Cvjetinović2021301,

title = {Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking},

author = {Đorđe Cvjetinović and Željko Prijović and Drina Janković and Magdalena Radović and Marija Mirković and Zorana Milanović and Miloš Mojović and Đani Škalamera and Sanja Vranješ-Đurić},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102414049&doi=10.1016%2fj.jconrel.2021.03.006&partnerID=40&md5=e3d7f2072a0b004f8d726c2358b83d71},

doi = {10.1016/j.jconrel.2021.03.006},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Controlled Release},

volume = {332},

pages = {301 – 311},

abstract = {Liposomes are promising drug's delivery systems due to decreased toxicity of the liposome-encapsulated drug, but wider clinical application requires their more efficient tumor targeting with uptake, controlled drug release and higher shelf life. The unique metabolic characteristics of cancer cells based on higher demand for energy and therefore increased glucose utilization were exploited in the design of glucose modified liposomes (GML) with the aim to provide increased tumor targeting via glucose transporters and increased ability of drug delivery into tumor cells. Tumor accumulating potential of GML and non-glucose liposomes (NGL) were investigated on CT26 and LS174T tumor-bearing mice by simple and reliable radiotracer method using 177Lu as radioactive marker. Both liposomes, GML and NGL were radiolabeled in high radiolabeling yield, showing high in vitro stability in biological media, as the main prerequisite for the biodistribution studies. Tumors displayed significantly better accumulation of 177Lu-GML with the maximum uptake 6 h post-injection (5.8 ± 0.2%/g in LS174T tumor and 5.1 ± 0.5%/g in CT26 tumor), compared to negligible uptake of 177Lu-NGL (0.6 ± 0.1%/g in LS174T tumor and 0.9 ± 0.2%/g in CT26 tumor). Results of comparative biodistribution studies of 177Lu-NGL and 177Lu-GML indicate that increased accumulation of GML is enabled by glucose transporters and subsequent endocytosis, resulting in their prolonged retention in tumor tissues (up to 72 h). Direct radiolabeling of liposomes with 177Lu may be used not only for biodistribution studies using radiotracking, but also for cancer treatment. © 2021 Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Petrović20211097,

title = {Nickel oxide on mechanochemically synthesized TiO2–CeO2: photocatalytic and electrochemical activity},

author = {Srdjan Petrović and Miroslav Stanković and Stefan Pavlović and Zorica Mojović and Nenad Radić and Miloš Mojović and Ljiljana Rožić},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108609286&doi=10.1007%2fs11144-021-02014-8&partnerID=40&md5=77b077247e58e48489f41b8a9f6b6b14},

doi = {10.1007/s11144-021-02014-8},

year  = {2021},

date = {2021-01-01},

journal = {Reaction Kinetics, Mechanisms and Catalysis},

volume = {133},

number = {2},

pages = {1097 – 1110},

abstract = {This paper presents a new two-step method for the synthesis of NiO/TiO2–CeO2 semiconductors, which implies, the first, the mechanochemical synthesis of TiO2–CeO2 support, and the second is the doping of different NiO contents by chemical precipitation in an aqueous medium in an ambient atmosphere. Structural, optical, electrochemical and photocatalytic properties of NiO/TiO2–CeO2 semiconductors were examined. The influence of different NiO contents on the electrocatalytic activity of NiO/TiO2–CeO2 samples was investigated in the oxygen evolution reaction and the results showed that the samples with the lowest NiO content have the highest electroactivity. The photocatalytic activity was determined during the phenol decomposition process over the samples with different NiO:TiO2–CeO2 ratios and the best photocatalytic activity is observed for the sample with 4.1 mol% NiO. The sample with the lowest NiO content obtained by a relatively simple method is suitable for use both as an electrode material in the oxygen evolution reaction and as a photocatalyst in the phenol decomposition process. © 2021, Akadémiai Kiadó, Budapest, Hungary.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matijević20211087,

title = {Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles},

author = {Milica Matijević and Jelena Žakula and Lela Korićanac and Marija Radoičić and Xinyue Liang and Lan Mi and Jelena Filipović Tričković and Ana Valenta Šobot and Maja N. Stanković and Đura Nakarada and Miloš Mojović and Marijana Petković and Milutin Stepić and Maja D. Nešić},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85112557363&doi=10.1007%2fs43630-021-00082-2&partnerID=40&md5=13e1274c257164a0fd80c90cb86a6ad9},

doi = {10.1007/s43630-021-00082-2},

year  = {2021},

date = {2021-01-01},

journal = {Photochemical and Photobiological Sciences},

volume = {20},

number = {8},

pages = {1087 – 1098},

abstract = {Abstract: In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy. Graphic abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Grippo2021,

title = {Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes},

author = {Valentina Grippo and Milos Mojovic and Aleksandra Pavicevic and Martin Kabelac and Frantisek Hubatka and Jaroslav Turanek and Martina Zatloukalova and Bruce A. Freeman and Jan Vacek},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096158093&doi=10.1016%2fj.redox.2020.101756&partnerID=40&md5=2da8f31c4fb805691910ec16078ae42b},

doi = {10.1016/j.redox.2020.101756},

year  = {2021},

date = {2021-01-01},

journal = {Redox Biology},

volume = {38},

abstract = {Fatty acid nitroalkenes (NO2-FA) are endogenously-generated products of the reaction of metabolic and inflammatory-derived nitrogen dioxide (.NO2) with unsaturated fatty acids. These species mediate signaling actions and induce adaptive responses in preclinical models of inflammatory and metabolic diseases. The nitroalkene substituent possesses an electrophilic nature, resulting in rapid and reversible reactions with biological nucleophiles such as cysteine, thus supporting post-translational modifications (PTM) of proteins having susceptible nucleophilic centers. These reactions contribute to enzyme regulation, modulation of inflammation and cell proliferation and the regulation of gene expression responses. Herein, focus is placed on the reduction-oxidation (redox) characteristics and stability of specific NO2-FA regioisomers having biological and clinical relevance; nitro-oleic acid (NO2-OA), bis-allylic nitro-linoleic acid (NO2-LA) and the conjugated diene-containing nitro-conjugated linoleic acid (NO2-cLA). Cyclic and alternating-current voltammetry and chronopotentiometry were used to the study of reduction potentials of these NO2-FA. R–NO2 reduction was observed around −0.8 V (vs. Ag/AgCl/3 M KCl) and is related to relative NO2-FA electrophilicity. This reduction process could be utilized for the evaluation of NO2-FA stability in aqueous milieu, shown herein to be pH dependent. In addition, electron paramagnetic resonance (EPR) spectroscopy was used to define the stability of the nitroalkene moiety under aqueous conditions, specifically under conditions where nitric oxide (.NO) release could be detected. The experimental data were supported by density functional theory calculations using 6–311++G (d,p) basis set and B3LYP functional. Based on experimental and computational approaches, the relative electrophilicities of these NO2-FA are NO2-cLA >> NO2-LA > NO2-OA. Micellarization and vesiculation largely define these biophysical characteristics in aqueous, nucleophile-free conditions. At concentrations below the critical micellar concentration (CMC), monomeric NO2-FA predominate, while at greater concentrations a micellar phase consisting of self-assembled lipid structures predominates. The CMC, determined by dynamic light scattering in 0.1 M phosphate buffer (pH 7.4) at 25 °C, was 6.9 (NO2-LA) 10.6 (NO2-OA) and 42.3 μM (NO2-cLA), respectively. In aggregate, this study provides new insight into the biophysical properties of NO2-FA that are important for better understanding the cell signaling and pharmacological potential of this class of mediators. © 2020 The Authors},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Krunić2021167,

title = {Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death},

author = {Matija Krunić and Biljana Ristić and Mihajlo Bošnjak and Verica Paunović and Gordana Tovilović-Kovačević and Nevena Zogović and Aleksandar Mirčić and Zoran Marković and Biljana Todorović-Marković and Svetlana Jovanović and Duška Kleut and Miloš Mojović and Đura Nakarada and Olivera Marković and Irena Vuković and Ljubica Harhaji-Trajković and Vladimir Trajković},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117824181&doi=10.1016%2fj.freeradbiomed.2021.10.025&partnerID=40&md5=dca6b01a062016c9894c25afee0a0369},

doi = {10.1016/j.freeradbiomed.2021.10.025},

year  = {2021},

date = {2021-01-01},

journal = {Free Radical Biology and Medicine},

volume = {177},

pages = {167 – 180},

abstract = {We investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP). GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•−), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagy-limiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy. © 2021},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Mojović2021,

title = {Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection},

author = {Zorica Mojović and Srdjan Petrović and Miloš Mojović and Stefan Pavlović and Ljiljana Rožić},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089660184&doi=10.1016%2fj.jpcs.2020.109649&partnerID=40&md5=2178ca9e6a6a97bf29af47c7530f08d5},

doi = {10.1016/j.jpcs.2020.109649},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Physics and Chemistry of Solids},

volume = {148},

abstract = {In this paper, the relationship of structure, type of oxygen defects, valence states of Cr and Ru, and electrochemical properties of La0.7Sr0.3Cr1-XRuXO3 (0 < x < 0.1) perovskite-type oxides were studied. The samples were synthesized by a conventional solid-state reaction method and characterized by powder X-ray diffraction, X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and electron paramagnetic resonance spectroscopy. The electrochemical performance of these samples was evaluated towards the detection of 4-nitrophenol and strongly depends on the concentration of ruthenium incorporated in the crystal lattice. A new La0.7Sr0.3Cr0.925Ru0.075O3 electrocatalyst with a wide linear concentration range (25–5000 mM) and a detection limit of 8 mM was obtained. These results provide an opportunity for its application in the analysis of real water samples. © 2020 Elsevier Ltd},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{DragišićMaksimović2021818,

title = {Spatial distribution of apoplastic antioxidative constituents in maize root},

author = {Jelena Dragišić Maksimović and Miloš Mojović and Željko Vučinić and Vuk Maksimović},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108365280&doi=10.1111%2fppl.13476&partnerID=40&md5=25d74508b1a75aa19bf65104d3635b34},

doi = {10.1111/ppl.13476},

year  = {2021},

date = {2021-01-01},

journal = {Physiologia Plantarum},

volume = {173},

number = {3},

pages = {818 – 828},

abstract = {Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showed a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial–temporal changes in small regions of the intact root tissue. © 2021 Scandinavian Plant Physiology Society},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Vesković202125,

title = {In vivo/ex vivo EPR investigation of the brain redox status and blood-brain barrier integrity in the 5xFAD mouse model of Alzheimer's disease},

author = {Ana Vesković and Đura Nakarada and Aleksandra Pavićević and Bogomir Prokić and Milka Perović and Selma Kanazir and Ana Popović-Bijelić and Miloš Mojović},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104293047&doi=10.2174%2f1567205018666210324121156&partnerID=40&md5=49c60056dd19f58d7aebc09a3518eae1},

doi = {10.2174/1567205018666210324121156},

year  = {2021},

date = {2021-01-01},

journal = {Current Alzheimer Research},

volume = {18},

number = {1},

pages = {25 – 34},

abstract = {Background: Alzheimer’s disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the research focus from Aβ to other pathological features of the disease. Objective: The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. Methods: In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibiting different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectroscopy was employed to investigate the brain metal content. Results: This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-mediated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. Conclusion: The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neuronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spectroscopy application in preclinical studies of neurodegenerative diseases. © 2021 Bentham Science Publishers.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Mišurović2020,

title = {Magnetite nanoparticles-catalyzed synthesis of conductive poly(p-aminodiphenylamine)},

author = {Jana Mišurović and Miloš Mojović and Budimir Marjanović and Predrag Vulić and Gordana Ćirić-Marjanović},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091590368&doi=10.1016%2fj.synthmet.2020.116577&partnerID=40&md5=9d58d17505031d1fc2956c8449632aa1},

doi = {10.1016/j.synthmet.2020.116577},

year  = {2020},

date = {2020-01-01},

journal = {Synthetic Metals},

volume = {269},

abstract = {Para-aminodiphenylamine (p-ADPA) was successfully polymerized by simple, economical and environmentally improved procedure. Magnetite nanoparticles (Fe3O4 NPs) were found to successfully catalyze the oxidation of p-ADPA in the presence of hydrogen peroxide (H2O2), an eco-friendly and clean oxidant, with a very small amount of ammonium peroxydisulfate (APS) as an initiator. In this manner, the amount of by-products occurring during the reaction is drastically reduced. The oxidation of p-ADPA proceeded also in the absence of Fe3O4 NPs, but far more slowly than the catalyzed process. The influence of the mass ratio Fe3O4NPs/p-ADPA on the properties of products was systematically studied by different characterization techniques. Spectroscopic measurements (UV-Vis-NIR, FTIR, Raman, EPR) indicate that the polymerization products are polyaniline emeraldine salt-like polymers and their measured electrical conductivity is one of the highest values reported in the literature for p-ADPA oligomerization/polymerization products (1.8∙10-2 S cm-1). X-ray powder diffraction (XRPD) measurements revealed highly crystalline structure of Poly(p-ADPA) samples synthesized with Fe3O4 NPs, while scanning electron microscopy (SEM) showed layered and prismatic-shaped particles as prevailing in their morphology. © 2020 Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Dimić20201,

title = {An experimental and theoretical study of the reactivity of selected catecholamines and their precursors towards ascorbyl radical},

author = {Dušan Dimić and Dura Nakarada and Miloš Mojović and Jasmina Dimitrić Marković},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089268212&doi=10.24874%2fjsscm.2020.01.01&partnerID=40&md5=53a5048f1ed5fbd05fef983f7cc340fc},

doi = {10.24874/jsscm.2020.01.01},

year  = {2020},

date = {2020-01-01},

journal = {Journal of the Serbian Society for Computational Mechanics},

number = {SpecialIssue},

pages = {1 – 12},

abstract = {Ascorbyl radical is often used as a biomarker of oxidative stress in human organism. It is a product of the antioxidant activity of ascorbic acid and it is not expected to react further with biologically important molecules. For the first time, the reactivity of catecholamines and their precursors was investigated theoretically and experimentally towards ascorbyl radical and the main structural parameters governing activity were analyzed. It was shown that catechol moiety plays an important role, which classifies norepinephrine and 3,4-dihydroxyphenylacetic acid as the most reactive when compared to homovanillic acid, vanillylmandelic acid, and octopamine. DFT methods have been employed to determine the most probable mechanism of the reaction. Based on the change in reaction enthalpy it was concluded that Hydrogen Atom Transfer (HAT) is a preferred mechanism both in water and pentyl ethanoate. The stabilization interactions in ascorbic acid, its anion and radical are carefully analyzed in order to understand the preferability of the mentioned mechanism. The stability of the ascorbyl radical is explained in detail. The results prove that ascorbyl radical is not just a product of antioxidant activity, but also a potentially harmful species in an organism. © 2020, Serbian Society of Computational Mechanics.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Carević20206820,

title = {Effect of Fe3+ ion doping on photocatalytic ability of nanozirconia ceramic to degrade 2, 4, 6- trichlorophenol},

author = {Milica V. Carević and Tatjana D. Savić and Nadica D. Abazović and Miloš D. Mojović and Tatjana B. Novaković and Mirjana I. Čomor},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078751596&doi=10.1016%2fj.ceramint.2019.11.175&partnerID=40&md5=1d7e4c1e95c26cae0304d07dcdf02f98},

doi = {10.1016/j.ceramint.2019.11.175},

year  = {2020},

date = {2020-01-01},

journal = {Ceramics International},

volume = {46},

number = {5},

pages = {6820 – 6827},

abstract = {Pure and a series of Fe3+ doped zirconia nanopowders were synthesized combining colloidal chemistry and solvothermal method from organometallic precursors in order to correlate doping and properties of zirconia matrix. After annealing of washed nanopowders at 600 °C, detailed characterization was performed using X-ray diffraction, UV/Vis absorption and luminescence, infrared and electron paramagnetic spectroscopy, transmission electron microscopy and BET measurements. Zirconia samples had mixed monoclinic and tetragonal crystalline phase; increasing Fe3+ ions concentration was followed by increasing of tetragonal phase share. In the sample with highest Fe3+ concentration, hematite can be detected. Also, UV/Vis spectrophotometry showed that Fe3+ doping lowers effective band gap of zirconia matrix from 4.5 eV (bulk value) to 2.1 eV for doped/nanocomposite samples. EPR measurements proved presence of dopant and showed that isolated Fe3+ ions in zirconia matrix exist in both crystalline phases; monoclinic and tetragonal (g ~ 4.8 and g ~ 4.27–4.2, respectively) surroundings. Zirconia samples were also successfully used as photocatalysts for photocatalytic degradation of 2, 4, 6, trichlorophenol. © 2019 Elsevier Ltd and Techna Group S.r.l.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Zang2020363,

title = {Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula},

author = {Lili Zang and Marie-Christine Morère-Le Paven and Thibault Clochard and Alexis Porcher and Pascale Satour and Miloš Mojović and Marija Vidović and Anis M. Limami and Françoise Montrichard},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075562315&doi=10.1016%2fj.plaphy.2019.11.006&partnerID=40&md5=8ce9220ea6bedb365cdc4b0f2655e7fa},

doi = {10.1016/j.plaphy.2019.11.006},

year  = {2020},

date = {2020-01-01},

journal = {Plant Physiology and Biochemistry},

volume = {146},

pages = {363 – 373},

abstract = {In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O2 •− → H2O2 → •OH) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O2 •−, H2O2 and •OH in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O2 •− accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into •OH. These changes impair the accumulation of H2O2 and then the accumulation of •OH, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway. © 2019 Elsevier Masson SAS},

keywords = {},

pubstate = {published},

tppubtype = {article}

}