Please select year or author:
2009
Kukavica, B.; Mojović, M.; Vučinić, Ž.; Maksimović, V.; Takahama, U.; Jovanović, S. V.
In: Plant and Cell Physiology, vol. 50, no. 2, pp. 304-317, 2009, (cited By 69).
Abstract | Links | BibTeX | Tags:
@article{Kukavica2009304,
title = {Generation of hydroxyl radical in isolated pea root cell wall, and the role of cell wall-bound peroxidase, Mn-SOD and phenolics in their production},
author = {B. Kukavica and M. Mojović and Ž. Vučinić and V. Maksimović and U. Takahama and S. V. Jovanović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-60149094200&doi=10.1093%2fpcp%2fpcn199&partnerID=40&md5=3a1745eef0e87e4a70c9a99913b2002f},
doi = {10.1093/pcp/pcn199},
year = {2009},
date = {2009-01-01},
journal = {Plant and Cell Physiology},
volume = {50},
number = {2},
pages = {304-317},
abstract = {The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H2O2 formation from dioxygen; and (ii) the POD-catalyzed reduction of H2O2 to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (·OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate ·OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electrophoresis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5- methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between ·OH and the superoxide radical (O 2-·). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPO/OH adduct formation. A decrease in DEPMPO/OH adduct formation in the presence of H 2O2 scavengers demonstrated that this hydroxyl radical was derived from H2O2. During the generation of ·OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H2O2 required for the formation of ·OH in isolated cell walls is produced during the reduction of O2 by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce ·OH. Addition of exogenous H2O2 again induced the production of ·OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions. However, the appearance of the DEPMPO/OOH adduct could also be observed, due to the production of O 2-· when endogenous SOD has been inactivated. Also, O2-· was converted to ·OH in an in vitro horseradish peroxidase (HRP)/H2O2 system to which exogenous SOD has been added. Taken together with the discovery of the cell wall-bound Mn-SOD isoform, these results support the role of such a cell wall-bound SOD in the formation of ·OH jointly with the cell wall-bound POD. According to the above findings, it seems that the hydroxycinnamic acids from the cell wall, acting as reductants, contribute to the formation of H 2O2 in the presence of O2 in an autocatalytic manner, and that POD and Mn-SOD coupled together generate ·OH from such H2O2. © The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.},
note = {cited By 69},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Spasojević, I.; Mojović, M.; Blagojević, D.; Spasić, S. D.; Jones, D. R.; Nikolić-Kokić, A.; Spasić, M. B.
Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical Journal Article
In: Carbohydrate Research, vol. 344, no. 1, pp. 80-84, 2009, (cited By 52).
Abstract | Links | BibTeX | Tags:
@article{Spasojević200980,
title = {Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical},
author = {I. Spasojević and M. Mojović and D. Blagojević and S. D. Spasić and D. R. Jones and A. Nikolić-Kokić and M. B. Spasić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-57749111966&doi=10.1016%2fj.carres.2008.09.025&partnerID=40&md5=efd9e15381559ca758d6e1449d776864},
doi = {10.1016/j.carres.2008.09.025},
year = {2009},
date = {2009-01-01},
journal = {Carbohydrate Research},
volume = {344},
number = {1},
pages = {80-84},
abstract = {The hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy. © 2008 Elsevier Ltd. All rights reserved.},
note = {cited By 52},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Kojic, D.; Spasojevic, I.; Mojovic, M.; Blagojevic, D.; Worland, M. Roger; Grubor-Lajsic, G.; Spasic, M. B.
Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae) Journal Article
In: European Journal of Entomology, vol. 106, no. 3, pp. 451-454, 2009, (cited By 17).
Abstract | Links | BibTeX | Tags:
@article{Kojic2009451,
title = {Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)},
author = {D. Kojic and I. Spasojevic and M. Mojovic and D. Blagojevic and M. Roger Worland and G. Grubor-Lajsic and M. B. Spasic},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-71949101615&doi=10.14411%2feje.2009.056&partnerID=40&md5=b5b45070bc766cdb874fdaa92f016b71},
doi = {10.14411/eje.2009.056},
year = {2009},
date = {2009-01-01},
journal = {European Journal of Entomology},
volume = {106},
number = {3},
pages = {451-454},
publisher = {Czech Academy of Sciences},
abstract = {The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5°C, -3°C and -16°C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3°C than at 5°C and -16°C. At the same temperature (-3°C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3°C, which is the temperature that triggers freezing tolerance, is suggested. © 2003 Institute of Entomology.},
note = {cited By 17},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Daković, M.; Mojović, M.; Bačić, G.
EPR study of the production of OH radicals in aqueous solutions of uranium irradiated by ultraviolet light [Russian Source] Journal Article
In: Journal of the Serbian Chemical Society, vol. 74, no. 6, pp. 651-661, 2009, (cited By 1).
Abstract | Links | BibTeX | Tags:
@article{Daković2009651,
title = {EPR study of the production of OH radicals in aqueous solutions of uranium irradiated by ultraviolet light [Russian Source]},
author = {M. Daković and M. Mojović and G. Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-68949085650&doi=10.2298%2fJSC0906651D&partnerID=40&md5=d74ca1b3fba8dca9bbfd34904aebb7b9},
doi = {10.2298/JSC0906651D},
year = {2009},
date = {2009-01-01},
journal = {Journal of the Serbian Chemical Society},
volume = {74},
number = {6},
pages = {651-661},
publisher = {Serbian Chemical Society},
abstract = {The aim of the study was to establish whether hydroxyl radicals (•OH) were produced in UV-irradiated aqueous solutions of uranyl salts. The production of •OH was studied in uranyl acetate and nitrate solutions by an EPR spin trap method over a wide pH range, with variation of the uranium concentrations. The production of •OH in uranyl solutions irradiated with UV was unequivocally demonstrated for the first time using the EPR spin-trapping method. The production of •OH can be connected to speciation of uranium species in aqueous solutions, showing a complex dependence on the solution pH. When compared with the results of radiative de-excitation of excited uranyl (* UO22+) by the quenching of its fluorescence, the present results indicate that the generation of hydroxyl radicals plays a major role in the fluorescence decay of * UO22+. The role of the presence of carbonates and counter ions pertinent to environmental conditions in biological systems on the production of hydroxyl radicals was also assessed in an attempt to reveal the mechanism of * UO22+ de-excitation. Various mechanisms, including •OH production, are inferred but the main point is that the generation of •OH in uranium containing solutions must be considered when assessing uranium toxicity. Copyright CC(2009) SCS.},
note = {cited By 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Daković, Marko; Mojović, Miloš; Bačić, Goran
EPR study of the production of OH radicals in aqueous solutions of uranium irradiated by ultraviolet light; [Russian Source] Journal Article
In: Journal of the Serbian Chemical Society, vol. 74, no. 6, pp. 651 – 661, 2009, (Cited by: 1; All Open Access, Bronze Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Daković2009651b,
title = {EPR study of the production of OH radicals in aqueous solutions of uranium irradiated by ultraviolet light; [Russian Source]},
author = {Marko Daković and Miloš Mojović and Goran Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-68949085650&doi=10.2298%2fJSC0906651D&partnerID=40&md5=d74ca1b3fba8dca9bbfd34904aebb7b9},
doi = {10.2298/JSC0906651D},
year = {2009},
date = {2009-01-01},
journal = {Journal of the Serbian Chemical Society},
volume = {74},
number = {6},
pages = {651 – 661},
abstract = {The aim of the study was to establish whether hydroxyl radicals (•OH) were produced in UV-irradiated aqueous solutions of uranyl salts. The production of •OH was studied in uranyl acetate and nitrate solutions by an EPR spin trap method over a wide pH range, with variation of the uranium concentrations. The production of •OH in uranyl solutions irradiated with UV was unequivocally demonstrated for the first time using the EPR spin-trapping method. The production of •OH can be connected to speciation of uranium species in aqueous solutions, showing a complex dependence on the solution pH. When compared with the results of radiative de-excitation of excited uranyl (* UO22+) by the quenching of its fluorescence, the present results indicate that the generation of hydroxyl radicals plays a major role in the fluorescence decay of * UO22+. The role of the presence of carbonates and counter ions pertinent to environmental conditions in biological systems on the production of hydroxyl radicals was also assessed in an attempt to reveal the mechanism of * UO22+ de-excitation. Various mechanisms, including •OH production, are inferred but the main point is that the generation of •OH in uranium containing solutions must be considered when assessing uranium toxicity. Copyright CC(2009) SCS.},
note = {Cited by: 1; All Open Access, Bronze Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Živković, Jelena; Zeković, Zoran; Mujić, Ibrahim; Signevac, Dejan Gocrossed D; Mojović, Miloš; Mujić, Aida; Spasojević, Ivan
In: Food Biophysics, vol. 4, no. 2, pp. 126 – 133, 2009, (Cited by: 24).
Abstract | Links | BibTeX | Tags:
@article{Živković2009126b,
title = {EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: Example on extracts of catkin, leaves, and spiny burs of castanea sativa},
author = {Jelena Živković and Zoran Zeković and Ibrahim Mujić and Dejan Gocrossed D Signevac and Miloš Mojović and Aida Mujić and Ivan Spasojević},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67349213556&doi=10.1007%2fs11483-009-9109-8&partnerID=40&md5=396637db5c47c6c616ea572464c292d4},
doi = {10.1007/s11483-009-9109-8},
year = {2009},
date = {2009-01-01},
journal = {Food Biophysics},
volume = {4},
number = {2},
pages = {126 – 133},
abstract = {Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K + was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media. © 2009 Springer Science+Business Media, LLC.},
note = {Cited by: 24},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ciric-Marjanovic, Gordana; Dragičević, Ljiljana; Milojević, Maja; Mojović, Miloš; Mentus, Slavko; Dojčinović, Biljana; Marjanović, Budimir; Stejskal, Jaroslav
Synthesis and characterization of self-assembled polyaniline nanotubes/silica nanocomposites Journal Article
In: Journal of Physical Chemistry B, vol. 113, no. 20, pp. 7116 – 7127, 2009, (Cited by: 71).
Abstract | Links | BibTeX | Tags:
@article{Ciric-Marjanovic20097116b,
title = {Synthesis and characterization of self-assembled polyaniline nanotubes/silica nanocomposites},
author = {Gordana Ciric-Marjanovic and Ljiljana Dragičević and Maja Milojević and Miloš Mojović and Slavko Mentus and Biljana Dojčinović and Budimir Marjanović and Jaroslav Stejskal},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67650065013&doi=10.1021%2fjp900096b&partnerID=40&md5=cda45850184db3f9f2c87f17aff96fee},
doi = {10.1021/jp900096b},
year = {2009},
date = {2009-01-01},
journal = {Journal of Physical Chemistry B},
volume = {113},
number = {20},
pages = {7116 – 7127},
abstract = {Self-assembled semiconducting, paramagnetic polyaniline nanotubes have been synthesized by the oxidative polymerization of aniline with ammonium peroxydisulfate in aqueous medium in the presence of colloidal silica particles of an average diameter ∼12 nm, without added acid. The electrical conductivity of polyaniline nanotubes/silica nanocomposites is in the range (3.3-4.0) × 10-3 S cm-1. The presence of paramagnetic polaronic emeraldine salt form of polyaniline and phenazine units in nanocomposites was proved by FTIR, Raman, and EPR spectroscopies. The influence of the initial silica/aniline weight ratio on the morphology of polyaniline/silica nanocomposites was studied by scanning and transmission electron microscopies. Nano-composites synthesized by using the initial weight ratio silica/aniline ≤0.2 contain polyaniline nanotubes which have a typical outer diameter of 100-250 nm and an inner diameter of 10-80 nm, and nanorods with a diameter of 60-100 nm, accompanied with polyaniline/silica nanogranules, while the nanocomposite synthesized at weight ratio silica/aniline ∼2 contains polyaniline/silica nanogranules with an average diameter of 35-70 nm. The evolution of molecular and supramolecular structure of polyaniline in the presence of colloidal silica is discussed. © 2009 American Chemical Society.},
note = {Cited by: 71},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Kukavica, Biljana; Mojović, Miloš; Vučinić, Željko; Maksimović, Vuk; Takahama, Umeo; Jovanović, Sonja Veljović
In: Plant and Cell Physiology, vol. 50, no. 2, pp. 304 – 317, 2009, (Cited by: 73; All Open Access, Bronze Open Access).
Abstract | Links | BibTeX | Tags:
@article{Kukavica2009304b,
title = {Generation of hydroxyl radical in isolated pea root cell wall, and the role of cell wall-bound peroxidase, Mn-SOD and phenolics in their production},
author = {Biljana Kukavica and Miloš Mojović and Željko Vučinić and Vuk Maksimović and Umeo Takahama and Sonja Veljović Jovanović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-60149094200&doi=10.1093%2fpcp%2fpcn199&partnerID=40&md5=3a1745eef0e87e4a70c9a99913b2002f},
doi = {10.1093/pcp/pcn199},
year = {2009},
date = {2009-01-01},
journal = {Plant and Cell Physiology},
volume = {50},
number = {2},
pages = {304 – 317},
abstract = {The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H2O2 formation from dioxygen; and (ii) the POD-catalyzed reduction of H2O2 to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (·OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate ·OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electrophoresis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5- methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between ·OH and the superoxide radical (O 2-·). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPO/OH adduct formation. A decrease in DEPMPO/OH adduct formation in the presence of H 2O2 scavengers demonstrated that this hydroxyl radical was derived from H2O2. During the generation of ·OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H2O2 required for the formation of ·OH in isolated cell walls is produced during the reduction of O2 by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce ·OH. Addition of exogenous H2O2 again induced the production of ·OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions. However, the appearance of the DEPMPO/OOH adduct could also be observed, due to the production of O 2-· when endogenous SOD has been inactivated. Also, O2-· was converted to ·OH in an in vitro horseradish peroxidase (HRP)/H2O2 system to which exogenous SOD has been added. Taken together with the discovery of the cell wall-bound Mn-SOD isoform, these results support the role of such a cell wall-bound SOD in the formation of ·OH jointly with the cell wall-bound POD. According to the above findings, it seems that the hydroxycinnamic acids from the cell wall, acting as reductants, contribute to the formation of H 2O2 in the presence of O2 in an autocatalytic manner, and that POD and Mn-SOD coupled together generate ·OH from such H2O2. © The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.},
note = {Cited by: 73; All Open Access, Bronze Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2008
Filipović, M. R.; Duerr, K.; Mojović, M.; Simeunović, V.; Zimmermann, R.; Niketić, V.; Ivanović-Burmazović, I.
NO dismutase activity of seven-coordinate manganese(II) pentaazamacrocyclic complexes Journal Article
In: Angewandte Chemie - International Edition, vol. 47, no. 45, pp. 8735-8739, 2008, (cited By 28).
Abstract | Links | BibTeX | Tags:
@article{Filipović20088735,
title = {NO dismutase activity of seven-coordinate manganese(II) pentaazamacrocyclic complexes},
author = {M. R. Filipović and K. Duerr and M. Mojović and V. Simeunović and R. Zimmermann and V. Niketić and I. Ivanović-Burmazović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-55249091126&doi=10.1002%2fanie.200801325&partnerID=40&md5=9ce584f68a0e9cf9f27296e391311168},
doi = {10.1002/anie.200801325},
year = {2008},
date = {2008-01-01},
journal = {Angewandte Chemie - International Edition},
volume = {47},
number = {45},
pages = {8735-8739},
abstract = {Redox selectivity? Seven-coordinate manganese(II) pentaazamacrocyclic complexes stimulate NO disproportionation by a novel dismutation mechanism based on the formation of labile metal-nitrosyl adducts and which is associated with the MnII/MnIII redox cycle. The metal-bound NO in these aducts has the character and reactivity of NO- and NO+ species. Ex vivo studies suggest that superoxide dismutase mimics of this kind could interfere with NO-mediated processes in biological milieu. (Chemical Equation Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.},
note = {cited By 28},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bogdanović, J.; Mojović, M.; Milosavić, N.; Mitrović, A.; Vučinić, Ž.; Spasojević, I.
Role of fructose in the adaptation of plants to cold-induced oxidative stress Journal Article
In: European Biophysics Journal, vol. 37, no. 7, pp. 1241-1246, 2008, (cited By 46).
Abstract | Links | BibTeX | Tags:
@article{Bogdanović20081241,
title = {Role of fructose in the adaptation of plants to cold-induced oxidative stress},
author = {J. Bogdanović and M. Mojović and N. Milosavić and A. Mitrović and Ž. Vučinić and I. Spasojević},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-50449105987&doi=10.1007%2fs00249-008-0260-9&partnerID=40&md5=025a55921bc130426ef8ff55eaed5332},
doi = {10.1007/s00249-008-0260-9},
year = {2008},
date = {2008-01-01},
journal = {European Biophysics Journal},
volume = {37},
number = {7},
pages = {1241-1246},
abstract = {This work presents findings, which indicate important role of fructose, fructose 6-phosphate (F6P), and fructose 1,6-bisphosphate (FBP) in preservation of homeostasis in plants under low temperature. Cold combined with light is known to incite increased generation of superoxide in chloroplasts leading to photoinhibition, but also an increased level of soluble sugars. In the present study, oxidative stress in pea leaves provoked by cold/light regime was asserted by the observed decrease of the level of oxidized form of PSI pigment P700 (P700+). Alongside, the increased antioxidative status and the accumulation of fructose were observed. The antioxidative properties of fructose and its phosphorylated forms were evaluated to appraise their potential protective role in plants exposed to chilling stress. Fructose, and particularly F6P and FBP exhibited high capacities for scavenging superoxide and showed to be involved in antioxidative protection in pea leaves. These results combined with previously established links implicate that the increase in level of fructose sugars through various pathways intercalated into physiological mechanisms of homeostasis represents important non-enzymatic antioxidative defense in plants under cold-related stress. © 2008 EBSA.},
note = {cited By 46},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bačić, G.; Spasojević, I.; Šećerov, B.; Mojović, M.
Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities Journal Article
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, vol. 69, no. 5, pp. 1354-1366, 2008, (cited By 79).
Abstract | Links | BibTeX | Tags:
@article{Bačić20081354,
title = {Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities},
author = {G. Bačić and I. Spasojević and B. Šećerov and M. Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-42149158251&doi=10.1016%2fj.saa.2007.09.047&partnerID=40&md5=f41fe03eec3fadb649a427f1029d8d4a},
doi = {10.1016/j.saa.2007.09.047},
year = {2008},
date = {2008-01-01},
journal = {Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy},
volume = {69},
number = {5},
pages = {1354-1366},
abstract = {The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (radical dotH) atom. © 2007 Elsevier B.V. All rights reserved.},
note = {cited By 79},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Godevac, D.; Vujisić, L.; Mojović, M.; Ignjatović, A.; Spasojević, I.; Vajs, V.
Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity Journal Article
In: Food Chemistry, vol. 107, no. 4, pp. 1692-1700, 2008, (cited By 53).
Abstract | Links | BibTeX | Tags:
@article{Godevac20081692,
title = {Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity},
author = {D. Godevac and L. Vujisić and M. Mojović and A. Ignjatović and I. Spasojević and V. Vajs},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-36448978506&doi=10.1016%2fj.foodchem.2007.10.017&partnerID=40&md5=8d3b102026c429cf7918f62d79d34886},
doi = {10.1016/j.foodchem.2007.10.017},
year = {2008},
date = {2008-01-01},
journal = {Food Chemistry},
volume = {107},
number = {4},
pages = {1692-1700},
abstract = {A total of 20 components were identified in Allium ursinum volatile oil (AUVO) by GC-MS, and 10 of them are found for the first time in this plant species. The antioxidant capacity of AUVO was examined by β-carotene-linoleic acid bleaching, lipid peroxidation by Fenton reaction, EPR spin-probing assay using stable nitroxide radicals, DPPHradical dot, and ABTSradical dot+ scavenging assays. Reaction mechanism of the volatile oil components with nitroxide radicals, based on IR spectra analysis, is proposed. AUVO demonstrated poor scavenging ability against DPPHradical dot and ABTSradical dot+ comparing to synthetic antioxidants BHT and trolox, while in β-carotene-linoleic acid system AUVO showed an effect comparable to those for BHT. AUVO was also capable to scavenge stable nitroxide radicals such as water-soluble Tempone, and 7-DS and 12-DS, incorporated into the liposome membrane. Finally, AUVO increased membrane fluidity, which could be an important feature for further in vivo investigation of some disorders, such as hypertension and atherosclerosis. © 2007 Elsevier Ltd. All rights reserved.},
note = {cited By 53},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bogdanović, J.; Mojović, M.; Milosavić, N.; Mitrović, A.; Vučinić, Ž.; Spasojević, I.
Role of fructose in the adaptation of plants to cold-induced oxidative stress Journal Article
In: European Biophysics Journal, vol. 37, no. 7, pp. 1241 – 1246, 2008, (Cited by: 52).
Abstract | Links | BibTeX | Tags:
@article{Bogdanović20081241b,
title = {Role of fructose in the adaptation of plants to cold-induced oxidative stress},
author = {J. Bogdanović and M. Mojović and N. Milosavić and A. Mitrović and Ž. Vučinić and I. Spasojević},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-50449105987&doi=10.1007%2fs00249-008-0260-9&partnerID=40&md5=025a55921bc130426ef8ff55eaed5332},
doi = {10.1007/s00249-008-0260-9},
year = {2008},
date = {2008-01-01},
journal = {European Biophysics Journal},
volume = {37},
number = {7},
pages = {1241 – 1246},
abstract = {This work presents findings, which indicate important role of fructose, fructose 6-phosphate (F6P), and fructose 1,6-bisphosphate (FBP) in preservation of homeostasis in plants under low temperature. Cold combined with light is known to incite increased generation of superoxide in chloroplasts leading to photoinhibition, but also an increased level of soluble sugars. In the present study, oxidative stress in pea leaves provoked by cold/light regime was asserted by the observed decrease of the level of oxidized form of PSI pigment P700 (P700+). Alongside, the increased antioxidative status and the accumulation of fructose were observed. The antioxidative properties of fructose and its phosphorylated forms were evaluated to appraise their potential protective role in plants exposed to chilling stress. Fructose, and particularly F6P and FBP exhibited high capacities for scavenging superoxide and showed to be involved in antioxidative protection in pea leaves. These results combined with previously established links implicate that the increase in level of fructose sugars through various pathways intercalated into physiological mechanisms of homeostasis represents important non-enzymatic antioxidative defense in plants under cold-related stress. © 2008 EBSA.},
note = {Cited by: 52},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Godevac, Dejan; Vujisić, Ljubodrag; Mojović, Miloš; Ignjatović, Aleksandar; Spasojević, Ivan; Vajs, Vlatka
Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity Journal Article
In: Food Chemistry, vol. 107, no. 4, pp. 1692 – 1700, 2008, (Cited by: 54).
Abstract | Links | BibTeX | Tags:
@article{Godevac20081692b,
title = {Evaluation of antioxidant capacity of Allium ursinum L. volatile oil and its effect on membrane fluidity},
author = {Dejan Godevac and Ljubodrag Vujisić and Miloš Mojović and Aleksandar Ignjatović and Ivan Spasojević and Vlatka Vajs},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-36448978506&doi=10.1016%2fj.foodchem.2007.10.017&partnerID=40&md5=8d3b102026c429cf7918f62d79d34886},
doi = {10.1016/j.foodchem.2007.10.017},
year = {2008},
date = {2008-01-01},
journal = {Food Chemistry},
volume = {107},
number = {4},
pages = {1692 – 1700},
abstract = {A total of 20 components were identified in Allium ursinum volatile oil (AUVO) by GC-MS, and 10 of them are found for the first time in this plant species. The antioxidant capacity of AUVO was examined by β-carotene-linoleic acid bleaching, lipid peroxidation by Fenton reaction, EPR spin-probing assay using stable nitroxide radicals, DPPHradical dot, and ABTSradical dot+ scavenging assays. Reaction mechanism of the volatile oil components with nitroxide radicals, based on IR spectra analysis, is proposed. AUVO demonstrated poor scavenging ability against DPPHradical dot and ABTSradical dot+ comparing to synthetic antioxidants BHT and trolox, while in β-carotene-linoleic acid system AUVO showed an effect comparable to those for BHT. AUVO was also capable to scavenge stable nitroxide radicals such as water-soluble Tempone, and 7-DS and 12-DS, incorporated into the liposome membrane. Finally, AUVO increased membrane fluidity, which could be an important feature for further in vivo investigation of some disorders, such as hypertension and atherosclerosis. © 2007 Elsevier Ltd. All rights reserved.},
note = {Cited by: 54},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bačić, Goran; Spasojević, Ivan; Šećerov, Bojana; Mojović, Miloš
Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities Journal Article
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, vol. 69, no. 5, pp. 1354 – 1366, 2008, (Cited by: 82).
Abstract | Links | BibTeX | Tags:
@article{Bačić20081354b,
title = {Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities},
author = {Goran Bačić and Ivan Spasojević and Bojana Šećerov and Miloš Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-42149158251&doi=10.1016%2fj.saa.2007.09.047&partnerID=40&md5=f41fe03eec3fadb649a427f1029d8d4a},
doi = {10.1016/j.saa.2007.09.047},
year = {2008},
date = {2008-01-01},
journal = {Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy},
volume = {69},
number = {5},
pages = {1354 – 1366},
abstract = {The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (radical dotH) atom. © 2007 Elsevier B.V. All rights reserved.},
note = {Cited by: 82},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Filipović, Miloš R.; Duerr, Katharina; Mojović, Miloš; Simeunović, Vladica; Zimmermann, Robert; Niketić, Vesna; Ivanović-Burmazović, Ivana
NO dismutase activity of seven-coordinate manganese(II) pentaazamacrocyclic complexes Journal Article
In: Angewandte Chemie - International Edition, vol. 47, no. 45, pp. 8735 – 8739, 2008, (Cited by: 28).
Abstract | Links | BibTeX | Tags:
@article{Filipović20088735b,
title = {NO dismutase activity of seven-coordinate manganese(II) pentaazamacrocyclic complexes},
author = {Miloš R. Filipović and Katharina Duerr and Miloš Mojović and Vladica Simeunović and Robert Zimmermann and Vesna Niketić and Ivana Ivanović-Burmazović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-55249091126&doi=10.1002%2fanie.200801325&partnerID=40&md5=9ce584f68a0e9cf9f27296e391311168},
doi = {10.1002/anie.200801325},
year = {2008},
date = {2008-01-01},
journal = {Angewandte Chemie - International Edition},
volume = {47},
number = {45},
pages = {8735 – 8739},
abstract = {Redox selectivity? Seven-coordinate manganese(II) pentaazamacrocyclic complexes stimulate NO disproportionation by a novel dismutation mechanism based on the formation of labile metal-nitrosyl adducts and which is associated with the MnII/MnIII redox cycle. The metal-bound NO in these aducts has the character and reactivity of NO- and NO+ species. Ex vivo studies suggest that superoxide dismutase mimics of this kind could interfere with NO-mediated processes in biological milieu. (Chemical Equation Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.},
note = {Cited by: 28},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2006
Maksimović, Vuk; Mojović, Miloš; Vučinić, Željko
Monosaccharide-H 2 O 2 reactions as a source of glycolate and their stimulation by hydroxyl radicals Journal Article
In: Carbohydrate Research, vol. 341, no. 14, pp. 2360 – 2369, 2006, (Cited by: 18; All Open Access, Bronze Open Access).
Abstract | Links | BibTeX | Tags:
@article{Maksimović20062360b,
title = {Monosaccharide-H 2 O 2 reactions as a source of glycolate and their stimulation by hydroxyl radicals},
author = {Vuk Maksimović and Miloš Mojović and Željko Vučinić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747801093&doi=10.1016%2fj.carres.2006.06.023&partnerID=40&md5=f26dbe11f9acbf8a464e76e446e12a98},
doi = {10.1016/j.carres.2006.06.023},
year = {2006},
date = {2006-01-01},
journal = {Carbohydrate Research},
volume = {341},
number = {14},
pages = {2360 – 2369},
abstract = {An analysis of the H 2 O 2 -induced breakdown and transformation of different keto-monosaccharides at physiological concentrations reveals that glycolate and other short-chained carbohydrates and organic acids are produced. Depletion of monosaccharides and glycolate synthesis occurs at increased rates as the length of the carbohydrate chain is decreased, and is significantly increased in the presence of trace amounts of Fe 2+ ions (10 μM). Rates of monosaccharide depletion (initial concentration of 3 mM) observed were up to 1.55 mmol h -1 in the case of fructose, and 2.59 mmol h -1 in the case of dihydroxyacetone, depending upon pH, H 2 O 2 concentration, temperature and the presence or absence of catalytic amounts of Fe 2+ . Glycolate was produced by dihydroxyacetone cleavage at rates up to 0.45 mmol h -1 in the absence, and up to 1.88 mmol h -1 in the presence of Fe 2+ ions (pH 8). Besides glycolate, other sugars (ribose, glyceraldehyde, glucose), glucitol (sorbitol) and organic acids (formic and 2-oxogluconic acid) were produced in such H 2 O 2 -induced reactions with fructose or dihydroxyacetone. EPR measurements demonstrated the participation of the radical dot OH radical, especially at higher pH. Presence of metal ions at higher pH values, resulting in increased glycolate synthesis, was accompanied by enhanced hydroxyl radical generation. Observed changes in intensity of DEPMPO-OH signals recorded from dihydroxyacetone and fructose reactions demonstrate a strong correlation with changes in glycolate yield, suggesting that radical dot OH radical formation enhances glycolate synthesis. The results presented suggest that different mechanisms are responsible for the cleavage or other reactions (isomerisation, auto- or free-radical-mediated oxidation) of keto-monosaccharides depending of experimental conditions. © 2006 Elsevier Ltd. All rights reserved.},
note = {Cited by: 18; All Open Access, Bronze Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Maksimović, V.; Mojović, M.; Vučinić, Z.
Monosaccharide-H 2 O 2 reactions as a source of glycolate and their stimulation by hydroxyl radicals Journal Article
In: Carbohydrate Research, vol. 341, no. 14, pp. 2360-2369, 2006, (cited By 18).
Abstract | Links | BibTeX | Tags:
@article{Maksimović20062360,
title = {Monosaccharide-H 2 O 2 reactions as a source of glycolate and their stimulation by hydroxyl radicals},
author = {V. Maksimović and M. Mojović and Z. Vučinić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747801093&doi=10.1016%2fj.carres.2006.06.023&partnerID=40&md5=f26dbe11f9acbf8a464e76e446e12a98},
doi = {10.1016/j.carres.2006.06.023},
year = {2006},
date = {2006-01-01},
journal = {Carbohydrate Research},
volume = {341},
number = {14},
pages = {2360-2369},
publisher = {Elsevier BV},
abstract = {An analysis of the H 2 O 2 -induced breakdown and transformation of different keto-monosaccharides at physiological concentrations reveals that glycolate and other short-chained carbohydrates and organic acids are produced. Depletion of monosaccharides and glycolate synthesis occurs at increased rates as the length of the carbohydrate chain is decreased, and is significantly increased in the presence of trace amounts of Fe 2+ ions (10 μM). Rates of monosaccharide depletion (initial concentration of 3 mM) observed were up to 1.55 mmol h -1 in the case of fructose, and 2.59 mmol h -1 in the case of dihydroxyacetone, depending upon pH, H 2 O 2 concentration, temperature and the presence or absence of catalytic amounts of Fe 2+ . Glycolate was produced by dihydroxyacetone cleavage at rates up to 0.45 mmol h -1 in the absence, and up to 1.88 mmol h -1 in the presence of Fe 2+ ions (pH 8). Besides glycolate, other sugars (ribose, glyceraldehyde, glucose), glucitol (sorbitol) and organic acids (formic and 2-oxogluconic acid) were produced in such H 2 O 2 -induced reactions with fructose or dihydroxyacetone. EPR measurements demonstrated the participation of the radical dot OH radical, especially at higher pH. Presence of metal ions at higher pH values, resulting in increased glycolate synthesis, was accompanied by enhanced hydroxyl radical generation. Observed changes in intensity of DEPMPO-OH signals recorded from dihydroxyacetone and fructose reactions demonstrate a strong correlation with changes in glycolate yield, suggesting that radical dot OH radical formation enhances glycolate synthesis. The results presented suggest that different mechanisms are responsible for the cleavage or other reactions (isomerisation, auto- or free-radical-mediated oxidation) of keto-monosaccharides depending of experimental conditions. © 2006 Elsevier Ltd. All rights reserved.},
note = {cited By 18},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2005
Mojović, M.; Spasojević, I.; Bačić, G.
Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems Journal Article
In: Journal of Chemical Information and Modeling, vol. 45, no. 6, pp. 1716-1718, 2005, (cited By 6).
Abstract | Links | BibTeX | Tags:
@article{Mojović20051716,
title = {Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems},
author = {M. Mojović and I. Spasojević and G. Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28944439903&doi=10.1021%2fci050173d&partnerID=40&md5=ad8614aa10785184910cfbae82e481df},
doi = {10.1021/ci050173d},
year = {2005},
date = {2005-01-01},
journal = {Journal of Chemical Information and Modeling},
volume = {45},
number = {6},
pages = {1716-1718},
publisher = {American Chemical Society},
abstract = {We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (*H) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bačič, G.; Mojović, M. Ann. N. Y. Acad. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (*H) adduct i.e., DEPMPO/H. An electrochemical system known to produce only *H radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems. © 2005 American Chemical Society.},
note = {cited By 6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Veljović-Jovanović, S.; Kukavica, B.; Cvetić, T.; Mojović, M.; Vučinić, Ž.
Ascorbic acid and the oxidative processes in pea root cell wall isolates: Characterization by fluorescence and EPR spectroscopy Journal Article
In: Annals of the New York Academy of Sciences, vol. 1048, pp. 500-504, 2005, (cited By 9).
Abstract | Links | BibTeX | Tags:
@article{Veljović-Jovanović2005500,
title = {Ascorbic acid and the oxidative processes in pea root cell wall isolates: Characterization by fluorescence and EPR spectroscopy},
author = {S. Veljović-Jovanović and B. Kukavica and T. Cvetić and M. Mojović and Ž. Vučinić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144470117&doi=10.1196%2fannals.1342.076&partnerID=40&md5=0d832399737d944722017d3ab82e5494},
doi = {10.1196/annals.1342.076},
year = {2005},
date = {2005-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1048},
pages = {500-504},
publisher = {New York Academy of Sciences},
abstract = {A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals. © 2005 New York Academy of Sciences.},
note = {cited By 9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}