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2020
Matijević, Milica; Nakarada, Đura; Liang, Xinyue; Korićanac, Lela; Rajsiglova, Lenka; Vannucci, Luca; Nešić, Maja; Vranješ, Mila; Mojović, Miloš; Mi, Lan; Estrela-Lopis, Irina; Böttner, Julia; Šaponjić, Zoran; Petković, Marijana; Stepić, Milutin
Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer Journal Article
In: Journal of Nanoparticle Research, vol. 22, no. 7, 2020.
Abstract | Links | BibTeX | Tags:
@article{Matijević2020,
title = {Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer},
author = {Milica Matijević and Đura Nakarada and Xinyue Liang and Lela Korićanac and Lenka Rajsiglova and Luca Vannucci and Maja Nešić and Mila Vranješ and Miloš Mojović and Lan Mi and Irina Estrela-Lopis and Julia Böttner and Zoran Šaponjić and Marijana Petković and Milutin Stepić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087054250&doi=10.1007%2fs11051-020-04899-3&partnerID=40&md5=9019709ed3fedee914b332555d4534ce},
doi = {10.1007/s11051-020-04899-3},
year = {2020},
date = {2020-01-01},
journal = {Journal of Nanoparticle Research},
volume = {22},
number = {7},
abstract = {TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study. © 2020, Springer Nature B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study. © 2020, Springer Nature B.V.
Zang, Lili; Paven, Marie-Christine Morère-Le; Clochard, Thibault; Porcher, Alexis; Satour, Pascale; Mojović, Miloš; Vidović, Marija; Limami, Anis M.; Montrichard, Françoise
Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula Journal Article
In: Plant Physiology and Biochemistry, vol. 146, pp. 363 – 373, 2020.
Abstract | Links | BibTeX | Tags:
@article{Zang2020363,
title = {Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula},
author = {Lili Zang and Marie-Christine Morère-Le Paven and Thibault Clochard and Alexis Porcher and Pascale Satour and Miloš Mojović and Marija Vidović and Anis M. Limami and Françoise Montrichard},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075562315&doi=10.1016%2fj.plaphy.2019.11.006&partnerID=40&md5=8ce9220ea6bedb365cdc4b0f2655e7fa},
doi = {10.1016/j.plaphy.2019.11.006},
year = {2020},
date = {2020-01-01},
journal = {Plant Physiology and Biochemistry},
volume = {146},
pages = {363 – 373},
abstract = {In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O2 •− → H2O2 → •OH) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O2 •−, H2O2 and •OH in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O2 •− accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into •OH. These changes impair the accumulation of H2O2 and then the accumulation of •OH, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway. © 2019 Elsevier Masson SAS},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O2 •− → H2O2 → •OH) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O2 •−, H2O2 and •OH in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O2 •− accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into •OH. These changes impair the accumulation of H2O2 and then the accumulation of •OH, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway. © 2019 Elsevier Masson SAS
Vujacic-Mirski, Ksenija; Bruns, Kai; Kalinovic, Sanela; Oelze, Matthias; Kröller-Schön, Swenja; Steven, Sebastian; Mojovic, Milos; Korac, Bato; Münzel, Thomas; Daiber, Andreas
In: Antioxidants, vol. 9, no. 5, 2020.
Abstract | Links | BibTeX | Tags:
@article{Vujacic-Mirski2020,
title = {Development of an analytical assay for electrochemical detection and quantification of protein-bound 3-nitrotyrosine in biological samples and comparison with classical, antibody-based methods},
author = {Ksenija Vujacic-Mirski and Kai Bruns and Sanela Kalinovic and Matthias Oelze and Swenja Kröller-Schön and Sebastian Steven and Milos Mojovic and Bato Korac and Thomas Münzel and Andreas Daiber},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084254228&doi=10.3390%2fantiox9050388&partnerID=40&md5=9144982ce1a9eea136a9663ecf80df32},
doi = {10.3390/antiox9050388},
year = {2020},
date = {2020-01-01},
journal = {Antioxidants},
volume = {9},
number = {5},
abstract = {Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantified by electrochemical detection (ECD, CoulArray) and compared to classical methods, namely enzyme-linked immunosorbent assay (ELISA) and dot blot analysis using specific 3-NT antibodies. Calibration curves for authentic 3-NT (detection limit 10 nM) and a concentration-response pattern for 3-NT obtained from digested nitrated bovine serum albumin (BSA) were highly linear over a wide 3-NT concentration range. Also, ex vivo nitration of protein from heart, isolated mitochondria, and serum/plasma could be quantified using the HPLC/ECD method and was confirmed by LC-MS/MS. Of note, nitro-oxidative damage of mitochondria results in increased superoxide (O2 •–) formation rates (measured by dihydroethidium-based HPLC assay), pointing to a self-amplification mechanism of oxidative stress. Based on our ex vivo data, the CoulArray quantification method for 3-NT seems to have some advantages regarding sensitivity and selectivity. Establishing a reliable automated HPLC assay for the routine quantification of 3-NT in biological samples of cell culture, of animal and human origin seems to be more sophisticated than expected. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantified by electrochemical detection (ECD, CoulArray) and compared to classical methods, namely enzyme-linked immunosorbent assay (ELISA) and dot blot analysis using specific 3-NT antibodies. Calibration curves for authentic 3-NT (detection limit 10 nM) and a concentration-response pattern for 3-NT obtained from digested nitrated bovine serum albumin (BSA) were highly linear over a wide 3-NT concentration range. Also, ex vivo nitration of protein from heart, isolated mitochondria, and serum/plasma could be quantified using the HPLC/ECD method and was confirmed by LC-MS/MS. Of note, nitro-oxidative damage of mitochondria results in increased superoxide (O2 •–) formation rates (measured by dihydroethidium-based HPLC assay), pointing to a self-amplification mechanism of oxidative stress. Based on our ex vivo data, the CoulArray quantification method for 3-NT seems to have some advantages regarding sensitivity and selectivity. Establishing a reliable automated HPLC assay for the routine quantification of 3-NT in biological samples of cell culture, of animal and human origin seems to be more sophisticated than expected. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Carević, Milica V.; Savić, Tatjana D.; Abazović, Nadica D.; Mojović, Miloš D.; Novaković, Tatjana B.; Čomor, Mirjana I.
Effect of Fe3+ ion doping on photocatalytic ability of nanozirconia ceramic to degrade 2, 4, 6- trichlorophenol Journal Article
In: Ceramics International, vol. 46, no. 5, pp. 6820 – 6827, 2020.
Abstract | Links | BibTeX | Tags:
@article{Carević20206820,
title = {Effect of Fe3+ ion doping on photocatalytic ability of nanozirconia ceramic to degrade 2, 4, 6- trichlorophenol},
author = {Milica V. Carević and Tatjana D. Savić and Nadica D. Abazović and Miloš D. Mojović and Tatjana B. Novaković and Mirjana I. Čomor},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078751596&doi=10.1016%2fj.ceramint.2019.11.175&partnerID=40&md5=1d7e4c1e95c26cae0304d07dcdf02f98},
doi = {10.1016/j.ceramint.2019.11.175},
year = {2020},
date = {2020-01-01},
journal = {Ceramics International},
volume = {46},
number = {5},
pages = {6820 – 6827},
abstract = {Pure and a series of Fe3+ doped zirconia nanopowders were synthesized combining colloidal chemistry and solvothermal method from organometallic precursors in order to correlate doping and properties of zirconia matrix. After annealing of washed nanopowders at 600 °C, detailed characterization was performed using X-ray diffraction, UV/Vis absorption and luminescence, infrared and electron paramagnetic spectroscopy, transmission electron microscopy and BET measurements. Zirconia samples had mixed monoclinic and tetragonal crystalline phase; increasing Fe3+ ions concentration was followed by increasing of tetragonal phase share. In the sample with highest Fe3+ concentration, hematite can be detected. Also, UV/Vis spectrophotometry showed that Fe3+ doping lowers effective band gap of zirconia matrix from 4.5 eV (bulk value) to 2.1 eV for doped/nanocomposite samples. EPR measurements proved presence of dopant and showed that isolated Fe3+ ions in zirconia matrix exist in both crystalline phases; monoclinic and tetragonal (g ~ 4.8 and g ~ 4.27–4.2, respectively) surroundings. Zirconia samples were also successfully used as photocatalysts for photocatalytic degradation of 2, 4, 6, trichlorophenol. © 2019 Elsevier Ltd and Techna Group S.r.l.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pure and a series of Fe3+ doped zirconia nanopowders were synthesized combining colloidal chemistry and solvothermal method from organometallic precursors in order to correlate doping and properties of zirconia matrix. After annealing of washed nanopowders at 600 °C, detailed characterization was performed using X-ray diffraction, UV/Vis absorption and luminescence, infrared and electron paramagnetic spectroscopy, transmission electron microscopy and BET measurements. Zirconia samples had mixed monoclinic and tetragonal crystalline phase; increasing Fe3+ ions concentration was followed by increasing of tetragonal phase share. In the sample with highest Fe3+ concentration, hematite can be detected. Also, UV/Vis spectrophotometry showed that Fe3+ doping lowers effective band gap of zirconia matrix from 4.5 eV (bulk value) to 2.1 eV for doped/nanocomposite samples. EPR measurements proved presence of dopant and showed that isolated Fe3+ ions in zirconia matrix exist in both crystalline phases; monoclinic and tetragonal (g ~ 4.8 and g ~ 4.27–4.2, respectively) surroundings. Zirconia samples were also successfully used as photocatalysts for photocatalytic degradation of 2, 4, 6, trichlorophenol. © 2019 Elsevier Ltd and Techna Group S.r.l.
2019
Mojović, Zorica; Novaković, Tatjana; Mojović, Miloš; Barudžija, Tanja; Mitrić, Miodrag
Electrochemical and structural properties of Ni(II)-alumina composites as an annealing temperature function Journal Article
In: Science of Sintering, vol. 51, no. 3, pp. 339 – 351, 2019.
Abstract | Links | BibTeX | Tags:
@article{Mojović2019339,
title = {Electrochemical and structural properties of Ni(II)-alumina composites as an annealing temperature function},
author = {Zorica Mojović and Tatjana Novaković and Miloš Mojović and Tanja Barudžija and Miodrag Mitrić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073755580&doi=10.2298%2fSOS1903339M&partnerID=40&md5=1378fd3c58eb5c654b04be2b551f6f94},
doi = {10.2298/SOS1903339M},
year = {2019},
date = {2019-01-01},
journal = {Science of Sintering},
volume = {51},
number = {3},
pages = {339 – 351},
abstract = {Alumina powders, pure and doped with nickel, were synthetized by sol-gel method and calcined at 500, 900 and 1100oC in order to obtain mesoporous structures with a high specific surface area, well adapTab. to catalytic application. The characterization of samples was performed by XRD, EPR spectroscopy and electrochemical impedance spectroscopy (EIS). XRD analysis showed that the addition of Ni2+, as well as the annealing temperature, affects the structural properties of the obtained composites. EPR analysis revealed the traces of Fe3+ impurities, the presence of oxy defects in alumina and Ni2+ in tetrahedral position for samples calcined at 1100oC. The impedance of the Nafion/alumina modified GCE depended on combined effect of porous structure and surface properties of alumina samples. The electrochemical behavior of a glassy carbon electrode modified with Ni (II)-doped aluminas was studied in 0.5 M NaOH solution, with and without methanol. The electrochemical activity of nickel-doped alumina composites was dictated by the amount of present NiO impurity. © 2018 Authors.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Alumina powders, pure and doped with nickel, were synthetized by sol-gel method and calcined at 500, 900 and 1100oC in order to obtain mesoporous structures with a high specific surface area, well adapTab. to catalytic application. The characterization of samples was performed by XRD, EPR spectroscopy and electrochemical impedance spectroscopy (EIS). XRD analysis showed that the addition of Ni2+, as well as the annealing temperature, affects the structural properties of the obtained composites. EPR analysis revealed the traces of Fe3+ impurities, the presence of oxy defects in alumina and Ni2+ in tetrahedral position for samples calcined at 1100oC. The impedance of the Nafion/alumina modified GCE depended on combined effect of porous structure and surface properties of alumina samples. The electrochemical behavior of a glassy carbon electrode modified with Ni (II)-doped aluminas was studied in 0.5 M NaOH solution, with and without methanol. The electrochemical activity of nickel-doped alumina composites was dictated by the amount of present NiO impurity. © 2018 Authors.
Mišurović, Jana; Mojović, Miloš; Marjanović, Budimir; Vulić, Predrag; Ćirić-Marjanović, Gordana
Magnetite nanoparticles-catalysed synthesis of conductive polyaniline Journal Article
In: Synthetic Metals, vol. 257, 2019.
Abstract | Links | BibTeX | Tags:
@article{Mišurović2019,
title = {Magnetite nanoparticles-catalysed synthesis of conductive polyaniline},
author = {Jana Mišurović and Miloš Mojović and Budimir Marjanović and Predrag Vulić and Gordana Ćirić-Marjanović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072565581&doi=10.1016%2fj.synthmet.2019.116174&partnerID=40&md5=92bb2ed38ff6e8a6b54636036aa5a80a},
doi = {10.1016/j.synthmet.2019.116174},
year = {2019},
date = {2019-01-01},
journal = {Synthetic Metals},
volume = {257},
abstract = {Nowadays, there is a growing interest to find environmentally-friendly, simple and economical routes for the synthesis of polyaniline (PANI), one of the most important conducting polymers. For the oxidative polymerization approach hydrogen peroxide (H2O2) is highly recommended oxidant due to ecological aspects, since the product of its reduction is only water. However, its usage as an oxidant, aimed to produce conductive PANI with reasonable reaction rate, needs a catalyst. Here we report the preparation of conductive PANI in good yield by the oxidative polymerization of aniline using magnetite nanoparticles (Fe3O4 NPs) as a catalyst and H2O2 as an oxidant, with added very small amount of ammonium peroxydisulfate as the initiator. Synthesized Fe3O4 NPs (size 10–50 nm) were characterized by X-ray powder diffraction (XRPD) and scanning electron microscopy (SEM). It was found that without Fe3O4 NPs, under otherwise same conditions, the reaction product is not PANI. The effect of mass ratio Fe3O4 NPs/aniline and polymerization time on the structure and physicochemical properties of prepared PANI was studied by various techniques. FTIR, Raman, UV–vis-NIR and electrical conductivity measurements confirmed the existence of conducting emeraldine salt form of PANI. XRPD revealed unchanged crystalline structure of Fe3O4 NPs upon polymerization and semi-crystalline structure of PANI. EPR spectra showed peaks assigned to PANI and Fe3O4 NPs. Described simple, environmentally improved synthetic route opens possibilities for the syntheses of conductive PANI-like polymers from other aromatic amines by using environmentally-friendly oxidant H2O2 and Fe3O4 NPs catalyst which can replace more expensive catalysts such as peroxidase enzymes. © 2019 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Nowadays, there is a growing interest to find environmentally-friendly, simple and economical routes for the synthesis of polyaniline (PANI), one of the most important conducting polymers. For the oxidative polymerization approach hydrogen peroxide (H2O2) is highly recommended oxidant due to ecological aspects, since the product of its reduction is only water. However, its usage as an oxidant, aimed to produce conductive PANI with reasonable reaction rate, needs a catalyst. Here we report the preparation of conductive PANI in good yield by the oxidative polymerization of aniline using magnetite nanoparticles (Fe3O4 NPs) as a catalyst and H2O2 as an oxidant, with added very small amount of ammonium peroxydisulfate as the initiator. Synthesized Fe3O4 NPs (size 10–50 nm) were characterized by X-ray powder diffraction (XRPD) and scanning electron microscopy (SEM). It was found that without Fe3O4 NPs, under otherwise same conditions, the reaction product is not PANI. The effect of mass ratio Fe3O4 NPs/aniline and polymerization time on the structure and physicochemical properties of prepared PANI was studied by various techniques. FTIR, Raman, UV–vis-NIR and electrical conductivity measurements confirmed the existence of conducting emeraldine salt form of PANI. XRPD revealed unchanged crystalline structure of Fe3O4 NPs upon polymerization and semi-crystalline structure of PANI. EPR spectra showed peaks assigned to PANI and Fe3O4 NPs. Described simple, environmentally improved synthetic route opens possibilities for the syntheses of conductive PANI-like polymers from other aromatic amines by using environmentally-friendly oxidant H2O2 and Fe3O4 NPs catalyst which can replace more expensive catalysts such as peroxidase enzymes. © 2019 Elsevier B.V.
Zatloukalova, Martina; Mojovic, Milos; Pavicevic, Aleksandra; Kabelac, Martin; Freeman, Bruce A.; Pekarova, Michaela; Vacek, Jan
Redox properties and human serum albumin binding of nitro-oleic acid Journal Article
In: Redox Biology, vol. 24, 2019.
Abstract | Links | BibTeX | Tags:
@article{Zatloukalova2019,
title = {Redox properties and human serum albumin binding of nitro-oleic acid},
author = {Martina Zatloukalova and Milos Mojovic and Aleksandra Pavicevic and Martin Kabelac and Bruce A. Freeman and Michaela Pekarova and Jan Vacek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066450363&doi=10.1016%2fj.redox.2019.101213&partnerID=40&md5=f04f807c490de0eab343cd1cc4201baf},
doi = {10.1016/j.redox.2019.101213},
year = {2019},
date = {2019-01-01},
journal = {Redox Biology},
volume = {24},
abstract = {Nitro-fatty acids modulate inflammatory and metabolic stress responses, thus displaying potential as new drug candidates. Herein, we evaluate the redox behavior of nitro-oleic acid (NO2-OA) and its ability to bind to the fatty acid transporter human serum albumin (HSA). The nitro group of NO2-OA underwent electrochemical reduction at −0.75 V at pH 7.4 in an aqueous milieu. Based on observations of the R–NO2 reduction process, the stability and reactivity of NO2-OA was measured in comparison to oleic acid (OA) as the negative control. These electrochemically-based results were reinforced by computational quantum mechanical modeling. DFT calculations indicated that both the C9-NO2 and C10-NO2 positional isomers of NO2-OA occurred in two conformers with different internal angles (69° and 110°) between the methyl- and carboxylate termini. Both NO2-OA positional isomers have LUMO energies of around −0.7 eV, affirming the electrophilic properties of fatty acid nitroalkenes. In addition, the binding of NO2-OA and OA with HSA revealed a molar ratio of ~7:1 [NO2-OA]:[HSA]. These binding experiments were performed using both an electrocatalytic approach and electron paramagnetic resonance (EPR) spectroscopy using 16-doxyl stearic acid. Using a Fe(DTCS)2 spin-trap, EPR studies also showed that the release of the nitro moiety of NO2-OA resulted in the formation of nitric oxide radical. Finally, the interaction of NO2-OA with HSA was monitored via Tyr and Trp residue electro-oxidation. The results indicate that not only non-covalent binding but also NO2-OA-HSA adduction mechanisms should be taken into consideration. This study of the redox properties of NO2-OA is applicable to the characterization of other electrophilic mediators of biological and pharmacological relevance. © 2019 The Authors},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Nitro-fatty acids modulate inflammatory and metabolic stress responses, thus displaying potential as new drug candidates. Herein, we evaluate the redox behavior of nitro-oleic acid (NO2-OA) and its ability to bind to the fatty acid transporter human serum albumin (HSA). The nitro group of NO2-OA underwent electrochemical reduction at −0.75 V at pH 7.4 in an aqueous milieu. Based on observations of the R–NO2 reduction process, the stability and reactivity of NO2-OA was measured in comparison to oleic acid (OA) as the negative control. These electrochemically-based results were reinforced by computational quantum mechanical modeling. DFT calculations indicated that both the C9-NO2 and C10-NO2 positional isomers of NO2-OA occurred in two conformers with different internal angles (69° and 110°) between the methyl- and carboxylate termini. Both NO2-OA positional isomers have LUMO energies of around −0.7 eV, affirming the electrophilic properties of fatty acid nitroalkenes. In addition, the binding of NO2-OA and OA with HSA revealed a molar ratio of ~7:1 [NO2-OA]:[HSA]. These binding experiments were performed using both an electrocatalytic approach and electron paramagnetic resonance (EPR) spectroscopy using 16-doxyl stearic acid. Using a Fe(DTCS)2 spin-trap, EPR studies also showed that the release of the nitro moiety of NO2-OA resulted in the formation of nitric oxide radical. Finally, the interaction of NO2-OA with HSA was monitored via Tyr and Trp residue electro-oxidation. The results indicate that not only non-covalent binding but also NO2-OA-HSA adduction mechanisms should be taken into consideration. This study of the redox properties of NO2-OA is applicable to the characterization of other electrophilic mediators of biological and pharmacological relevance. © 2019 The Authors
Marković, Srdjan Z.; Jovanović, Nina I. Dimitrijević; Sedić, Biserka; Vuković, Mira H.; Okjan, Jelena J.; Dimitrijević, Ivan K.; Trikoš, Lela D.; Mojović, Miloš D; Kastratović, Dragana A.
Impact of Differences in Economic Development and Socioeconomic Stability on Benzodiazepine Exposure between the Three Balkans Countries Journal Article
In: Psychiatria Danubina, vol. 31, pp. 750 – 760, 2019.
Abstract | Links | BibTeX | Tags:
@article{Marković2019750,
title = {Impact of Differences in Economic Development and Socioeconomic Stability on Benzodiazepine Exposure between the Three Balkans Countries},
author = {Srdjan Z. Marković and Nina I. Dimitrijević Jovanović and Biserka Sedić and Mira H. Vuković and Jelena J. Okjan and Ivan K. Dimitrijević and Lela D. Trikoš and Miloš D Mojović and Dragana A. Kastratović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081927165&partnerID=40&md5=1c1c2945da90f0446fc7464386c6b782},
year = {2019},
date = {2019-01-01},
journal = {Psychiatria Danubina},
volume = {31},
pages = {750 – 760},
abstract = {INTRODUCTION: Anxiety disorders are among the most common mental disorders. Benzodiazepines belong to the group of anxiolytic sedatives and the most prescribed drugs in the world. The aim in ours study was to evaluate the differences in the exposure of the population to benzodiazepines (in period from 2014-2018) between Serbia, Slovenia and Croatia, the three countries of the Southwestern Balkans with varying degrees of socioeconomic development. STUDY DESIGN: A academic investigator initiated, pharmacoepidemiological difference-in-difference time series analysis of population exposure to benzodiazepines between the three, geographically close Balkans countries (Slovenia, Serbia, Croatia) with varying degrees of socioeconomic development has been carried out. Study was conducted as academic investigator initiated, in a retrospective manner on monthly basis international data set from January 2014 to December 2018. RESULTS: At the annual level, during the study period from January 2014 to December 2018, compared to Slovenia, Serbia and Croatia had higher DIDs, from 5 fold (Croatia) to 6 fold (Serbia), for all benzodiazepines in total. By analyzing the differences-in-difference, we have shown that influence of both time (month) and country on DIDs is significant as well as their mutual interaction (the country x month) for all benzodiazepines in total. CONCLUSION: Serbia and Croatia must implement explicit measures of reducing benzodiazepine prescription in health primary care based on evidence-based recommendations in the indications where general medicine practitioners/family doctors most commonly prescribe these medicines. Without providing a realistic supplement/alternative to benzodiazepines such as increasing the availability of psychotherapy and improving the structure of psychiatric professionals in healthcare settings, implicit measures are not recommended for reducing prescription, implementing accountability measures for prolonged prescription of benzodiazepines, and in particular for "masked" somatic diseases. All this comes to the fore by raising economic development and socioeconomic stability.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
INTRODUCTION: Anxiety disorders are among the most common mental disorders. Benzodiazepines belong to the group of anxiolytic sedatives and the most prescribed drugs in the world. The aim in ours study was to evaluate the differences in the exposure of the population to benzodiazepines (in period from 2014-2018) between Serbia, Slovenia and Croatia, the three countries of the Southwestern Balkans with varying degrees of socioeconomic development. STUDY DESIGN: A academic investigator initiated, pharmacoepidemiological difference-in-difference time series analysis of population exposure to benzodiazepines between the three, geographically close Balkans countries (Slovenia, Serbia, Croatia) with varying degrees of socioeconomic development has been carried out. Study was conducted as academic investigator initiated, in a retrospective manner on monthly basis international data set from January 2014 to December 2018. RESULTS: At the annual level, during the study period from January 2014 to December 2018, compared to Slovenia, Serbia and Croatia had higher DIDs, from 5 fold (Croatia) to 6 fold (Serbia), for all benzodiazepines in total. By analyzing the differences-in-difference, we have shown that influence of both time (month) and country on DIDs is significant as well as their mutual interaction (the country x month) for all benzodiazepines in total. CONCLUSION: Serbia and Croatia must implement explicit measures of reducing benzodiazepine prescription in health primary care based on evidence-based recommendations in the indications where general medicine practitioners/family doctors most commonly prescribe these medicines. Without providing a realistic supplement/alternative to benzodiazepines such as increasing the availability of psychotherapy and improving the structure of psychiatric professionals in healthcare settings, implicit measures are not recommended for reducing prescription, implementing accountability measures for prolonged prescription of benzodiazepines, and in particular for "masked" somatic diseases. All this comes to the fore by raising economic development and socioeconomic stability.
Jović-Jovičić, N.; Mojović, M.; Stanković, D.; Nedić-Vasiljević, B.; Milutinović-Nikolić, A.; Banković, P.; Mojović, Z.
Characterization and electrochemical properties of organomodified and corresponding derived carbonized clay Journal Article
In: Electrochimica Acta, vol. 296, pp. 387 – 396, 2019.
Abstract | Links | BibTeX | Tags:
@article{Jović-Jovičić2019387,
title = {Characterization and electrochemical properties of organomodified and corresponding derived carbonized clay},
author = {N. Jović-Jovičić and M. Mojović and D. Stanković and B. Nedić-Vasiljević and A. Milutinović-Nikolić and P. Banković and Z. Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057196522&doi=10.1016%2fj.electacta.2018.11.031&partnerID=40&md5=e37babad2ffe275c442708b9c9908bc7},
doi = {10.1016/j.electacta.2018.11.031},
year = {2019},
date = {2019-01-01},
journal = {Electrochimica Acta},
volume = {296},
pages = {387 – 396},
abstract = {Series of alkylammonium modified smectites with different alkylammoinum/clay ratios was synthesized (H series). The obtained organoclays were used as precursors for carbon-clay composite materials (C series). Both set of materials were characterized by X-ray diffraction (XRD), point of zero charge (pHPZC), N2 adsorption-desorption isotherms, Raman spectroscopy and electron paramagnetic resonance (EPR) analysis. XRD analysis of organomodified clays confirmed incorporation of HDTMA+ into interlamellar space of smectite. Series of carbonized clays showed constant d001 value of 1.4 nm. The pHPZC of two series differed indicating that carbonization changed the profile of pH dependent sites on the clay surface. Textural properties of the H series decreased throughout the series, while in the C series the most developed porous structure was obtained for sample where HDTMA+ loading was equal to cation exchange capacity. Raman spectroscopy showed that amorphous carbon was formed during carbonization process. The obtained materials were used as modifiers of carbon paste electrode and investigated using electrochemical impedance spectroscopy, cyclic voltammetry and square wave voltammetry. The comparison of electrochemical behavior of H series and C series showed the importance of interlamellar species for charge transfer process. The investigation of influence of composition of carbon paste and form of added carbon showed that performance of carbon-clay based electrodes depended on synergy of different factors. © 2018 Elsevier Ltd},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Series of alkylammonium modified smectites with different alkylammoinum/clay ratios was synthesized (H series). The obtained organoclays were used as precursors for carbon-clay composite materials (C series). Both set of materials were characterized by X-ray diffraction (XRD), point of zero charge (pHPZC), N2 adsorption-desorption isotherms, Raman spectroscopy and electron paramagnetic resonance (EPR) analysis. XRD analysis of organomodified clays confirmed incorporation of HDTMA+ into interlamellar space of smectite. Series of carbonized clays showed constant d001 value of 1.4 nm. The pHPZC of two series differed indicating that carbonization changed the profile of pH dependent sites on the clay surface. Textural properties of the H series decreased throughout the series, while in the C series the most developed porous structure was obtained for sample where HDTMA+ loading was equal to cation exchange capacity. Raman spectroscopy showed that amorphous carbon was formed during carbonization process. The obtained materials were used as modifiers of carbon paste electrode and investigated using electrochemical impedance spectroscopy, cyclic voltammetry and square wave voltammetry. The comparison of electrochemical behavior of H series and C series showed the importance of interlamellar species for charge transfer process. The investigation of influence of composition of carbon paste and form of added carbon showed that performance of carbon-clay based electrodes depended on synergy of different factors. © 2018 Elsevier Ltd
Nakarada, Đura; Pejin, Boris; Dimić, Dušan; Ivanović-Šašić, Ana; Mojović, Zorica; Mojović, Miloš
Electrochemical and spectroscopic study of l-dopa interaction with avarol Journal Article
In: Reaction Kinetics, Mechanisms and Catalysis, vol. 127, no. 1, pp. 219 – 229, 2019.
Abstract | Links | BibTeX | Tags:
@article{Nakarada2019219,
title = {Electrochemical and spectroscopic study of l-dopa interaction with avarol},
author = {Đura Nakarada and Boris Pejin and Dušan Dimić and Ana Ivanović-Šašić and Zorica Mojović and Miloš Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064334278&doi=10.1007%2fs11144-019-01575-z&partnerID=40&md5=7fce655d8615f446eee5f3a78b1dfe3c},
doi = {10.1007/s11144-019-01575-z},
year = {2019},
date = {2019-01-01},
journal = {Reaction Kinetics, Mechanisms and Catalysis},
volume = {127},
number = {1},
pages = {219 – 229},
abstract = {The electrochemistry of catecholamine neurotransmitters and their precursor l-dopa has been widely studied due to their relevance as biologically important compounds. The detection of these compounds from aqueous solution is hindered by the coexistence of quinone or hydroquinone. However, it was suggested that quinones adsorbed on the electrode surface can enhance catechol detection. In order to estimate the degree of interaction between quinones and l-dopa, cyclic voltammetry and UV–Vis spectroscopic study was performed. A sesquiterpenoid hydroquinone, isolated from the marine sponge Dysidea avara (avarol), has been used in this study. The change of apparent heterogeneous rate constant with different avarol/l-dopa ratio indicated that charge transfer could be enhanced at some extent. In addition to this, the obtained results for avarol and hydroquinone (its structural element) were compared. UV–Vis spectroscopic analysis confirmed interaction between l-dopa and avarol or hydroquinone. Taken all together, the interaction of l-dopa was stronger with hydroquinone than with avarol, presumably reflecting the conformational restrains of avarol caused by its terpenoid moiety. © 2019, Akadémiai Kiadó, Budapest, Hungary.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The electrochemistry of catecholamine neurotransmitters and their precursor l-dopa has been widely studied due to their relevance as biologically important compounds. The detection of these compounds from aqueous solution is hindered by the coexistence of quinone or hydroquinone. However, it was suggested that quinones adsorbed on the electrode surface can enhance catechol detection. In order to estimate the degree of interaction between quinones and l-dopa, cyclic voltammetry and UV–Vis spectroscopic study was performed. A sesquiterpenoid hydroquinone, isolated from the marine sponge Dysidea avara (avarol), has been used in this study. The change of apparent heterogeneous rate constant with different avarol/l-dopa ratio indicated that charge transfer could be enhanced at some extent. In addition to this, the obtained results for avarol and hydroquinone (its structural element) were compared. UV–Vis spectroscopic analysis confirmed interaction between l-dopa and avarol or hydroquinone. Taken all together, the interaction of l-dopa was stronger with hydroquinone than with avarol, presumably reflecting the conformational restrains of avarol caused by its terpenoid moiety. © 2019, Akadémiai Kiadó, Budapest, Hungary.
2018
Egea, J.; Fabregat, I.; Frapart, Y. M.; Ghezzi, P.; Görlach, A.; Kietzmann, T.; Kubaichuk, K.; Knaus, U. G.; Lopez, M. G.; Olaso-Gonzalez, G.; Petry, A.; Schulz, R.; Vina, J.; Winyard, P.; Abbas, K.; Ademowo, O. S.; Afonso, C. B.; Andreadou, I.; Antelmann, H.; Antunes, F.; Aslan, M.; Bachschmid, M. M.; Barbosa, R. M.; Belousov, V.; Berndt, C.; Bernlohr, D.; Bertrán, E.; Bindoli, A.; Bottari, S. P.; Brito, P. M.; Carrara, G.; Casas, A. I.; Chatzi, A.; Chondrogianni, N.; Conrad, M.; Cooke, M. S.; Costa, J. G.; Cuadrado, A.; Dang, P. My-Chan; Smet, B. De; Debelec-Butuner, B.; Dias, I. H. K.; Dunn, J. D.; Edson, A. J.; Assar, M. El; El-Benna, J.; Ferdinandy, P.; Fernandes, A. S.; Fladmark, K. E.; Förstermann, U.; Giniatullin, R.; Giricz, Z.; Görbe, A.; Griffiths, H.; Hampl, V.; Hanf, A.; Herget, J.; Hernansanz-Agustín, P.; Hillion, M.; Huang, J.; Ilikay, S.; Jansen-Dürr, P.; Jaquet, V.; Joles, J. A.; Kalyanaraman, B.; Kaminskyy, D.; Karbaschi, M.; Kleanthous, M.; Klotz, L. O.; Korac, B.; Korkmaz, K. S.; Koziel, R.; Kračun, D.; Krause, K. H.; Křen, V.; Krieg, T.; Laranjinha, J.; Lazou, A.; Li, H.; Martínez-Ruiz, A.; Matsui, R.; McBean, G. J.; Meredith, S. P.; Messens, J.; Miguel, V.; Mikhed, Y.; Milisav, I.; Milković, L.; Miranda-Vizuete, A.; Mojović, M.; Monsalve, M.; Mouthuy, P. A.; Mulvey, J.; Münzel, T.; Muzykantov, V.; Nguyen, I. T. N.; Oelze, M.; Oliveira, N. G.; Palmeira, C. M.; Papaevgeniou, N.; Pavićević, A.; Pedre, B.; Peyrot, F.; Phylactides, M.; Pircalabioru, G. G.; Pitt, A. R.; Poulsen, H. E.; Prieto, I.; Rigobello, M. P.; Robledinos-Antón, N.; Rodríguez-Mañas, L.; Rolo, A. P.; Rousset, F.; Ruskovska, T.; Saraiva, N.; Sasson, S.; Schröder, K.; Semen, K.; Seredenina, T.; Shakirzyanova, A.; Smith, G. L.; Soldati, T.; Sousa, B. C.; Spickett, C. M.; Stancic, A.; Stasia, M. J.; Steinbrenner, H.; Stepanić, V.; Steven, S.; Tokatlidis, K.; Tuncay, E.; Turan, B.; Ursini, F.; Vacek, J.; Vajnerova, O.; Valentová, K.; Breusegem, F. Van; Varisli, L.; Veal, E. A.; Yalçın, A. S.; Yelisyeyeva, O.; Žarković, N.; Zatloukalová, M.; Zielonka, J.; Touyz, R. M.; Papapetropoulos, A.; Grune, T.; Lamas, S.; Schmidt, H. H. H. W.; Lisa, F. Di; Daiber, A.
In: Redox Biology, vol. 14, pp. 694 – 696, 2018.
Abstract | Links | BibTeX | Tags:
@article{Egea2018694,
title = {Corrigendum to “European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)” (Redox Biol. (2017) 13 (94–162)(S2213231717303373)(10.1016/j.redox.2017.05.007))},
author = {J. Egea and I. Fabregat and Y. M. Frapart and P. Ghezzi and A. Görlach and T. Kietzmann and K. Kubaichuk and U. G. Knaus and M. G. Lopez and G. Olaso-Gonzalez and A. Petry and R. Schulz and J. Vina and P. Winyard and K. Abbas and O. S. Ademowo and C. B. Afonso and I. Andreadou and H. Antelmann and F. Antunes and M. Aslan and M. M. Bachschmid and R. M. Barbosa and V. Belousov and C. Berndt and D. Bernlohr and E. Bertrán and A. Bindoli and S. P. Bottari and P. M. Brito and G. Carrara and A. I. Casas and A. Chatzi and N. Chondrogianni and M. Conrad and M. S. Cooke and J. G. Costa and A. Cuadrado and P. My-Chan Dang and B. De Smet and B. Debelec-Butuner and I. H. K. Dias and J. D. Dunn and A. J. Edson and M. El Assar and J. El-Benna and P. Ferdinandy and A. S. Fernandes and K. E. Fladmark and U. Förstermann and R. Giniatullin and Z. Giricz and A. Görbe and H. Griffiths and V. Hampl and A. Hanf and J. Herget and P. Hernansanz-Agustín and M. Hillion and J. Huang and S. Ilikay and P. Jansen-Dürr and V. Jaquet and J. A. Joles and B. Kalyanaraman and D. Kaminskyy and M. Karbaschi and M. Kleanthous and L. O. Klotz and B. Korac and K. S. Korkmaz and R. Koziel and D. Kračun and K. H. Krause and V. Křen and T. Krieg and J. Laranjinha and A. Lazou and H. Li and A. Martínez-Ruiz and R. Matsui and G. J. McBean and S. P. Meredith and J. Messens and V. Miguel and Y. Mikhed and I. Milisav and L. Milković and A. Miranda-Vizuete and M. Mojović and M. Monsalve and P. A. Mouthuy and J. Mulvey and T. Münzel and V. Muzykantov and I. T. N. Nguyen and M. Oelze and N. G. Oliveira and C. M. Palmeira and N. Papaevgeniou and A. Pavićević and B. Pedre and F. Peyrot and M. Phylactides and G. G. Pircalabioru and A. R. Pitt and H. E. Poulsen and I. Prieto and M. P. Rigobello and N. Robledinos-Antón and L. Rodríguez-Mañas and A. P. Rolo and F. Rousset and T. Ruskovska and N. Saraiva and S. Sasson and K. Schröder and K. Semen and T. Seredenina and A. Shakirzyanova and G. L. Smith and T. Soldati and B. C. Sousa and C. M. Spickett and A. Stancic and M. J. Stasia and H. Steinbrenner and V. Stepanić and S. Steven and K. Tokatlidis and E. Tuncay and B. Turan and F. Ursini and J. Vacek and O. Vajnerova and K. Valentová and F. Van Breusegem and L. Varisli and E. A. Veal and A. S. Yalçın and O. Yelisyeyeva and N. Žarković and M. Zatloukalová and J. Zielonka and R. M. Touyz and A. Papapetropoulos and T. Grune and S. Lamas and H. H. H. W. Schmidt and F. Di Lisa and A. Daiber},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032289195&doi=10.1016%2fj.redox.2017.10.001&partnerID=40&md5=55a7fd96e9207d6f19933a497cb8fd96},
doi = {10.1016/j.redox.2017.10.001},
year = {2018},
date = {2018-01-01},
journal = {Redox Biology},
volume = {14},
pages = {694 – 696},
abstract = {The authors regret that they have to correct the acknowledgement of the above mentioned publication as follows: This article/publication is based upon work from COST Action BM1203 (EU-ROS), supported by COST (European Cooperation in Science and Technology) which is funded by the Horizon 2020 Framework Programme of the European Union. COST (European Cooperation in Science and Technology) is a funding agency for research and innovation networks. Our Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation. For further information see www.cost.eu. The authors would like to apologise for any inconvenience caused. © 2017 The Author(s)},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The authors regret that they have to correct the acknowledgement of the above mentioned publication as follows: This article/publication is based upon work from COST Action BM1203 (EU-ROS), supported by COST (European Cooperation in Science and Technology) which is funded by the Horizon 2020 Framework Programme of the European Union. COST (European Cooperation in Science and Technology) is a funding agency for research and innovation networks. Our Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation. For further information see www.cost.eu. The authors would like to apologise for any inconvenience caused. © 2017 The Author(s)
Pavićević, Aleksandra; Lakočević, Milan; Popović, Milan; Popović-Bijelić, Ana; Daković, Marko; Mojović, Miloš
In: Biological Chemistry, vol. 399, no. 5, pp. 447 – 452, 2018.
Abstract | Links | BibTeX | Tags:
@article{Pavićević2018447,
title = {Changes of the peripheral blood mononuclear cells membrane fluidity from type 1 Gaucher disease patients: An electron paramagnetic resonance study},
author = {Aleksandra Pavićević and Milan Lakočević and Milan Popović and Ana Popović-Bijelić and Marko Daković and Miloš Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040229210&doi=10.1515%2fhsz-2017-0241&partnerID=40&md5=97ad367b49635a4af750c1d459d6a461},
doi = {10.1515/hsz-2017-0241},
year = {2018},
date = {2018-01-01},
journal = {Biological Chemistry},
volume = {399},
number = {5},
pages = {447 – 452},
abstract = {Gaucher disease (GD) is a lysosomal storage disorder, caused by an impaired function of β-glucocerebrosidase, which results in accumulation of glucocerebroside in cells, and altered membrane ordering. Using electron paramagnetic resonance spin labeling, a statistically significant difference in the order parameter between the peripheral blood mononuclear cell membranes of GD patients and healthy controls was observed. Moreover, the results show that the introduction of the enzyme replacement therapy leads to the restoration of the physiological membrane fluidity. Accordingly, this simple method could serve as a preliminary test for GD diagnosis and therapy efficiency. © 2018 Walter de Gruyter GmbH, Berlin/Boston 2018.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Gaucher disease (GD) is a lysosomal storage disorder, caused by an impaired function of β-glucocerebrosidase, which results in accumulation of glucocerebroside in cells, and altered membrane ordering. Using electron paramagnetic resonance spin labeling, a statistically significant difference in the order parameter between the peripheral blood mononuclear cell membranes of GD patients and healthy controls was observed. Moreover, the results show that the introduction of the enzyme replacement therapy leads to the restoration of the physiological membrane fluidity. Accordingly, this simple method could serve as a preliminary test for GD diagnosis and therapy efficiency. © 2018 Walter de Gruyter GmbH, Berlin/Boston 2018.
Carević, M. V.; Abazović, N. D.; Mitrić, M. N.; Ćirić-Marjanović, G.; Mojović, M. D.; Ahrenkiel, S. P.; Čomor, M. I.
Properties of Zirconia/Polyaniline hybrid nanocomposites and their application as photocatalysts for degradation of model pollutants Journal Article
In: Materials Chemistry and Physics, vol. 205, pp. 130 – 137, 2018.
Abstract | Links | BibTeX | Tags:
@article{Carević2018130,
title = {Properties of Zirconia/Polyaniline hybrid nanocomposites and their application as photocatalysts for degradation of model pollutants},
author = {M. V. Carević and N. D. Abazović and M. N. Mitrić and G. Ćirić-Marjanović and M. D. Mojović and S. P. Ahrenkiel and M. I. Čomor},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041460485&doi=10.1016%2fj.matchemphys.2017.11.016&partnerID=40&md5=1b2e005d16bce2153d75a67fdc4aa4ba},
doi = {10.1016/j.matchemphys.2017.11.016},
year = {2018},
date = {2018-01-01},
journal = {Materials Chemistry and Physics},
volume = {205},
pages = {130 – 137},
abstract = {A serie of novel ZrO2/Polyaniline (PANI) hybrid nanocomposite powders were successfully synthesized by the oxidative polymerization of aniline with ammonium peroxydisulfate (APS) in water, in the presence of ZrO2 nanoparticles. Syntheses were performed at initial ZrO2/aniline mole ratios 50, 100 and 150 to produce ZP-50, ZP-100 and ZP-150. The morphological, structural, and optical properties of the synthesized nanocomposites were studied using Transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Raman, UV-Vis and Electron-paramagnetic resonance (EPR) spectroscopies. EPR spectra of ZP-50 and ZP-100 nanocomposites showed the presence of intensive peak at the g-values comparable to that for free electrons (2.0023) indicating the presence of oligo/poly (semiquinone radical cations), i.e. polarons as charge carriers in PANI part; ZP-150 showed weak polaron peak of PANI, and peak that can be assigned to defects in zirconia matrix (Zr3+, oxygen vacancies). The monoclinic crystal structure of ZrO2 nanoparticles in all nanocomposites was confirmed by XRD and by Raman spectroscopy. The photocatalytic activities of ZP nanocomposites were evaluated using the photocatalytic degradation of trichlorophenol (TCP) and Rhodamine B model compounds and compared with the activity of bare ZrO2 nanoparticles. Enhanced degradation efficiencies of nanocomposites regarding TCP degradation were observed. © 2017 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A serie of novel ZrO2/Polyaniline (PANI) hybrid nanocomposite powders were successfully synthesized by the oxidative polymerization of aniline with ammonium peroxydisulfate (APS) in water, in the presence of ZrO2 nanoparticles. Syntheses were performed at initial ZrO2/aniline mole ratios 50, 100 and 150 to produce ZP-50, ZP-100 and ZP-150. The morphological, structural, and optical properties of the synthesized nanocomposites were studied using Transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Raman, UV-Vis and Electron-paramagnetic resonance (EPR) spectroscopies. EPR spectra of ZP-50 and ZP-100 nanocomposites showed the presence of intensive peak at the g-values comparable to that for free electrons (2.0023) indicating the presence of oligo/poly (semiquinone radical cations), i.e. polarons as charge carriers in PANI part; ZP-150 showed weak polaron peak of PANI, and peak that can be assigned to defects in zirconia matrix (Zr3+, oxygen vacancies). The monoclinic crystal structure of ZrO2 nanoparticles in all nanocomposites was confirmed by XRD and by Raman spectroscopy. The photocatalytic activities of ZP nanocomposites were evaluated using the photocatalytic degradation of trichlorophenol (TCP) and Rhodamine B model compounds and compared with the activity of bare ZrO2 nanoparticles. Enhanced degradation efficiencies of nanocomposites regarding TCP degradation were observed. © 2017 Elsevier B.V.
Novak, David; Mojovic, Milos; Pavicevic, Aleksandra; Zatloukalova, Martina; Hernychova, Lenka; Bartosik, Martin; Vacek, Jan
Electrochemistry and electron paramagnetic resonance spectroscopy of cytochrome c and its heme-disrupted analogs Journal Article
In: Bioelectrochemistry, vol. 119, pp. 136 – 141, 2018.
Abstract | Links | BibTeX | Tags:
@article{Novak2018136,
title = {Electrochemistry and electron paramagnetic resonance spectroscopy of cytochrome c and its heme-disrupted analogs},
author = {David Novak and Milos Mojovic and Aleksandra Pavicevic and Martina Zatloukalova and Lenka Hernychova and Martin Bartosik and Jan Vacek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030846989&doi=10.1016%2fj.bioelechem.2017.09.011&partnerID=40&md5=2e16f08089d8eb3a7a48776082daf510},
doi = {10.1016/j.bioelechem.2017.09.011},
year = {2018},
date = {2018-01-01},
journal = {Bioelectrochemistry},
volume = {119},
pages = {136 – 141},
abstract = {Cytochrome c (cyt c) is one of the most studied conjugated proteins due to its electron-transfer properties and ability to regulate the processes involved in homeostasis or apoptosis. Here we report an electrochemical strategy for investigating the electroactivity of cyt c and its analogs with a disrupted heme moiety, i.e. apocytochrome c (acyt c) and porphyrin cytochrome c (pcyt c). The electrochemical data are supplemented with low-temperature and spin-probe electron paramagnetic resonance (EPR) spectroscopy. The main contribution of this report is a complex evaluation of cyt c reduction and oxidation at the level of surface-localized amino acid residues and the heme moiety in a single electrochemical scan. The electrochemical pattern of cyt c is substantially different to both analogs acyt c and pcyt c, which could be applicable in further studies on the redox properties and structural stability of cytochromes and other hemeproteins. © 2017 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Cytochrome c (cyt c) is one of the most studied conjugated proteins due to its electron-transfer properties and ability to regulate the processes involved in homeostasis or apoptosis. Here we report an electrochemical strategy for investigating the electroactivity of cyt c and its analogs with a disrupted heme moiety, i.e. apocytochrome c (acyt c) and porphyrin cytochrome c (pcyt c). The electrochemical data are supplemented with low-temperature and spin-probe electron paramagnetic resonance (EPR) spectroscopy. The main contribution of this report is a complex evaluation of cyt c reduction and oxidation at the level of surface-localized amino acid residues and the heme moiety in a single electrochemical scan. The electrochemical pattern of cyt c is substantially different to both analogs acyt c and pcyt c, which could be applicable in further studies on the redox properties and structural stability of cytochromes and other hemeproteins. © 2017 Elsevier B.V.
2017
Stamenković, Stefan; Pavićević, Aleksandra; Mojović, Miloš; Popović-Bijelić, Ana; Selaković, Vesna; Andjus, Pavle; Bačić, Goran
In vivo EPR pharmacokinetic evaluation of the redox status and the blood brain barrier permeability in the SOD1G93A ALS rat model Journal Article
In: Free Radical Biology and Medicine, vol. 108, pp. 258 – 269, 2017.
Abstract | Links | BibTeX | Tags:
@article{Stamenković2017258,
title = {In vivo EPR pharmacokinetic evaluation of the redox status and the blood brain barrier permeability in the SOD1G93A ALS rat model},
author = {Stefan Stamenković and Aleksandra Pavićević and Miloš Mojović and Ana Popović-Bijelić and Vesna Selaković and Pavle Andjus and Goran Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016781498&doi=10.1016%2fj.freeradbiomed.2017.03.034&partnerID=40&md5=d8a9e1301fc55700e756d77bb4a81f64},
doi = {10.1016/j.freeradbiomed.2017.03.034},
year = {2017},
date = {2017-01-01},
journal = {Free Radical Biology and Medicine},
volume = {108},
pages = {258 – 269},
abstract = {Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder affecting the motor pathways of the central nervous system. Although a number of pathophysiological mechanisms have been described in the disease, post mortem and animal model studies indicate blood-brain barrier (BBB) disruption and elevated production of reactive oxygen species as major contributors to disease pathology. In this study, the BBB permeability and the brain tissue redox status of the SOD1G93A ALS rat model in the presymptomatic (preALS) and symptomatic (ALS) stages of the disease were investigated by in vivo EPR spectroscopy using three aminoxyl radicals with different cell membrane and BBB permeabilities, Tempol, 3-carbamoyl proxyl (3CP), and 3-carboxy proxyl (3CxP). Additionally, the redox status of the two brain regions previously implicated in disease pathology, brainstem and hippocampus, was investigated by spectrophotometric biochemical assays. The EPR results indicated that among the three spin probes, 3CP is the most suitable for reporting the intracellular redox status changes, as Tempol was reduced in vivo within minutes (t1/2 =2.0±0.5 min), thus preventing reliable kinetic modeling, whereas 3CxP reduction kinetics gave divergent conclusions, most probably due to its membrane impermeability. It was observed that the reduction kinetics of 3CP in vivo, in the head of preALS and ALS SOD1G93A rats was altered compared to the controls. Pharmacokinetic modeling of 3CP reduction in vivo, revealed elevated tissue distribution and tissue reduction rate constants indicating an altered brain tissue redox status, and possibly BBB disruption in these animals. The preALS and ALS brain tissue homogenates also showed increased nitrilation, superoxide production, lipid peroxidation and manganese superoxide dismutase activity, and a decreased copper-zinc superoxide dismutase activity. The present study highlights in vivo EPR spectroscopy as a reliable tool for the investigation of changes in BBB permeability and for the unprecedented in vivo monitoring of the brain tissue redox status, as early markers of ALS. © 2017 Elsevier Inc.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder affecting the motor pathways of the central nervous system. Although a number of pathophysiological mechanisms have been described in the disease, post mortem and animal model studies indicate blood-brain barrier (BBB) disruption and elevated production of reactive oxygen species as major contributors to disease pathology. In this study, the BBB permeability and the brain tissue redox status of the SOD1G93A ALS rat model in the presymptomatic (preALS) and symptomatic (ALS) stages of the disease were investigated by in vivo EPR spectroscopy using three aminoxyl radicals with different cell membrane and BBB permeabilities, Tempol, 3-carbamoyl proxyl (3CP), and 3-carboxy proxyl (3CxP). Additionally, the redox status of the two brain regions previously implicated in disease pathology, brainstem and hippocampus, was investigated by spectrophotometric biochemical assays. The EPR results indicated that among the three spin probes, 3CP is the most suitable for reporting the intracellular redox status changes, as Tempol was reduced in vivo within minutes (t1/2 =2.0±0.5 min), thus preventing reliable kinetic modeling, whereas 3CxP reduction kinetics gave divergent conclusions, most probably due to its membrane impermeability. It was observed that the reduction kinetics of 3CP in vivo, in the head of preALS and ALS SOD1G93A rats was altered compared to the controls. Pharmacokinetic modeling of 3CP reduction in vivo, revealed elevated tissue distribution and tissue reduction rate constants indicating an altered brain tissue redox status, and possibly BBB disruption in these animals. The preALS and ALS brain tissue homogenates also showed increased nitrilation, superoxide production, lipid peroxidation and manganese superoxide dismutase activity, and a decreased copper-zinc superoxide dismutase activity. The present study highlights in vivo EPR spectroscopy as a reliable tool for the investigation of changes in BBB permeability and for the unprecedented in vivo monitoring of the brain tissue redox status, as early markers of ALS. © 2017 Elsevier Inc.
Jović-Jovičić, Nataša; Mojović, Zorica; Mojović, Miloš; Banković, Predrag; Ajduković, Marija; Milutinović-Nikolić, Aleksandra; Jovanović, Dušan
Electrochemical behavior of immobilized hemoglobin in alkaline solution Journal Article
In: Applied Surface Science, vol. 400, pp. 347 – 354, 2017.
Abstract | Links | BibTeX | Tags:
@article{Jović-Jovičić2017347,
title = {Electrochemical behavior of immobilized hemoglobin in alkaline solution},
author = {Nataša Jović-Jovičić and Zorica Mojović and Miloš Mojović and Predrag Banković and Marija Ajduković and Aleksandra Milutinović-Nikolić and Dušan Jovanović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007609871&doi=10.1016%2fj.apsusc.2016.12.151&partnerID=40&md5=b5e41f54e1707025ac4caff1d20a6b2a},
doi = {10.1016/j.apsusc.2016.12.151},
year = {2017},
date = {2017-01-01},
journal = {Applied Surface Science},
volume = {400},
pages = {347 – 354},
abstract = {Glassy carbon electrode was modified with different synthesized hybrid clay-based materials and tested in alkaline solution with and without H 2 O 2 . The hybrid materials were obtained by immobilizing hemoglobin (Hb) on acid activated (AA) clay, or on AA clay modified with different sodium dodecyl sulfate (SDS) loadings. The obtained materials were characterized using DR UV–vis and ESR spectroscopy, elemental analysis, and SEM. The characterization confirmed higher degree of hemoglobin incorporation in the presence of SDS. The presence of SDS on the surface of clay particles resulted in the partial oxidation/denaturation of hemoglobin and formation of hemichrome. Cyclic voltammetry was used for the investigation of the electrochemical behavior of immobilized hemoglobin in alkaline solution. Two cathodic peaks at −0.45 V and −0.70 V were recorded and ascribed to the reduction of heme Fe(III)/Fe(II), and formation of HbFe(I) – highly reduced form of hemoglobin − respectively. The latter peak reflects hemoglobin denaturation. The presence of H 2 O 2 in the alkaline solution increased current intensities corresponding to both peaks (−0.45 V and −0.7 V). Linear response of peak current intensity vs. H 2 O 2 concentration was monitored for all investigated samples within different H 2 O 2 concentration ranges. The AA-SDS1.0-Hb electrode exhibited the highest current response with linear regression equation in the following form: I(μA) = 7.99 + 1.056 × [H 2 O 2 ] (mM) (R = 0.996). The limit of detection of 28 μM was estimated using the 3 sigma method. Different modified electrodes exhibited different degrees of denaturation resistance. The obtained values of Michaelis-Menten constant indicated that prolonged cycling in the presence of SDS increases protein denaturation. © 2016 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Glassy carbon electrode was modified with different synthesized hybrid clay-based materials and tested in alkaline solution with and without H 2 O 2 . The hybrid materials were obtained by immobilizing hemoglobin (Hb) on acid activated (AA) clay, or on AA clay modified with different sodium dodecyl sulfate (SDS) loadings. The obtained materials were characterized using DR UV–vis and ESR spectroscopy, elemental analysis, and SEM. The characterization confirmed higher degree of hemoglobin incorporation in the presence of SDS. The presence of SDS on the surface of clay particles resulted in the partial oxidation/denaturation of hemoglobin and formation of hemichrome. Cyclic voltammetry was used for the investigation of the electrochemical behavior of immobilized hemoglobin in alkaline solution. Two cathodic peaks at −0.45 V and −0.70 V were recorded and ascribed to the reduction of heme Fe(III)/Fe(II), and formation of HbFe(I) – highly reduced form of hemoglobin − respectively. The latter peak reflects hemoglobin denaturation. The presence of H 2 O 2 in the alkaline solution increased current intensities corresponding to both peaks (−0.45 V and −0.7 V). Linear response of peak current intensity vs. H 2 O 2 concentration was monitored for all investigated samples within different H 2 O 2 concentration ranges. The AA-SDS1.0-Hb electrode exhibited the highest current response with linear regression equation in the following form: I(μA) = 7.99 + 1.056 × [H 2 O 2 ] (mM) (R = 0.996). The limit of detection of 28 μM was estimated using the 3 sigma method. Different modified electrodes exhibited different degrees of denaturation resistance. The obtained values of Michaelis-Menten constant indicated that prolonged cycling in the presence of SDS increases protein denaturation. © 2016 Elsevier B.V.
Barudžija, Tanja; Cvjetićanin, Nikola; Bajuk-Bogdanović, Danica; Mojović, Miloš; Mitrić, Miodrag
Vibrational and electron paramagnetic resonance spectroscopic studies of β-MnO2 and α-KxMnO2 nanorods Journal Article
In: Journal of Alloys and Compounds, vol. 728, pp. 259 – 270, 2017.
Abstract | Links | BibTeX | Tags:
@article{Barudžija2017259,
title = {Vibrational and electron paramagnetic resonance spectroscopic studies of β-MnO2 and α-KxMnO2 nanorods},
author = {Tanja Barudžija and Nikola Cvjetićanin and Danica Bajuk-Bogdanović and Miloš Mojović and Miodrag Mitrić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028743086&doi=10.1016%2fj.jallcom.2017.09.015&partnerID=40&md5=73ee3765ffa02ccd8de20664bfcb56a6},
doi = {10.1016/j.jallcom.2017.09.015},
year = {2017},
date = {2017-01-01},
journal = {Journal of Alloys and Compounds},
volume = {728},
pages = {259 – 270},
abstract = {Raman scattering (RS), Fourier transform infrared (FTIR) and electron paramagnetic resonance (EPR) spectroscopic measurements on β-MnO2 and α-KxMnO2 nanorods were performed. The infrared spectrum of β-MnO2 is sensitive towards the particle sizes, while the Raman spectrum is sensitive towards the presence of nonstoichiometric and disordered MnO2-δ on the surface of nanorods. EPR measurements show the appearance of two types of Mn4+ ions having different local environment: (i) Mn4+ ions in Mn4+-rich environment and (ii) Mn4+ ions in mixed-valence Mn4+/Mn3+ environment that match up with region of nonstoichiometric MnO2-δ. Observed bands in vibrational spectra of tetragonal hollandite-type α-KxMnO2 are considered to be mainly due to the vibration modes of Mn[sbnd]O frameworks. The EPR spectra of α-KxMnO2 nanorods contain two signals that can be attributed to Mn4+ ions in Mn4+-rich environment and Mn4+ ions in mixed-valence Mn4+/Mn3+ environment close to K+ ions. © 2017 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Raman scattering (RS), Fourier transform infrared (FTIR) and electron paramagnetic resonance (EPR) spectroscopic measurements on β-MnO2 and α-KxMnO2 nanorods were performed. The infrared spectrum of β-MnO2 is sensitive towards the particle sizes, while the Raman spectrum is sensitive towards the presence of nonstoichiometric and disordered MnO2-δ on the surface of nanorods. EPR measurements show the appearance of two types of Mn4+ ions having different local environment: (i) Mn4+ ions in Mn4+-rich environment and (ii) Mn4+ ions in mixed-valence Mn4+/Mn3+ environment that match up with region of nonstoichiometric MnO2-δ. Observed bands in vibrational spectra of tetragonal hollandite-type α-KxMnO2 are considered to be mainly due to the vibration modes of Mn[sbnd]O frameworks. The EPR spectra of α-KxMnO2 nanorods contain two signals that can be attributed to Mn4+ ions in Mn4+-rich environment and Mn4+ ions in mixed-valence Mn4+/Mn3+ environment close to K+ ions. © 2017 Elsevier B.V.
Pavićević, Aleksandra; Luo, Jinghui; Popović-Bijelić, Ana; Mojović, Miloš
Maleimido-proxyl as an EPR spin label for the evaluation of conformational changes of albumin Journal Article
In: European Biophysics Journal, vol. 46, no. 8, pp. 773 – 787, 2017.
Abstract | Links | BibTeX | Tags:
@article{Pavićević2017773,
title = {Maleimido-proxyl as an EPR spin label for the evaluation of conformational changes of albumin},
author = {Aleksandra Pavićević and Jinghui Luo and Ana Popović-Bijelić and Miloš Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029763050&doi=10.1007%2fs00249-017-1257-z&partnerID=40&md5=6d642951aac69e88ed10d3d4123b8814},
doi = {10.1007/s00249-017-1257-z},
year = {2017},
date = {2017-01-01},
journal = {European Biophysics Journal},
volume = {46},
number = {8},
pages = {773 – 787},
abstract = {Albumin is the most abundant plasma protein and as such has been the subject of many studies using a variety of techniques. One of them, capable of monitoring the conformational changes and the binding capacity of proteins, is electron paramagnetic resonance spectroscopy (EPR) spin labeling. To date, albumin has been investigated using a number of different spin labels, mostly spin-labeled fatty acids (SLFAs). However, albumin can bind up to seven equivalents of fatty acids, making it difficult to determine which parts of the molecule undergo conformational changes. To obtain information from a specific site on a protein, spin labels that bind to free cysteine residues may be used. In this work, the applicability of such a label, 3-maleimido proxyl (5-MSL), was evaluated for monitoring conformational changes of bovine serum albumin (BSA) at different temperatures and pH values. Also, the effect of ethanol, reactive oxygen species (hydrogen peroxide and superoxide radical), and the binding of ligands specific for albumin, namely fatty acids, and several drugs were evaluated. The results indicate that the labeling of albumin at its free cysteine residue (Cys-34) using 5-MSL may successfully be used for the detection of conformational changes, even in the case of the subtle alterations induced by ligand binding. © 2017, European Biophysical Societies' Association.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Albumin is the most abundant plasma protein and as such has been the subject of many studies using a variety of techniques. One of them, capable of monitoring the conformational changes and the binding capacity of proteins, is electron paramagnetic resonance spectroscopy (EPR) spin labeling. To date, albumin has been investigated using a number of different spin labels, mostly spin-labeled fatty acids (SLFAs). However, albumin can bind up to seven equivalents of fatty acids, making it difficult to determine which parts of the molecule undergo conformational changes. To obtain information from a specific site on a protein, spin labels that bind to free cysteine residues may be used. In this work, the applicability of such a label, 3-maleimido proxyl (5-MSL), was evaluated for monitoring conformational changes of bovine serum albumin (BSA) at different temperatures and pH values. Also, the effect of ethanol, reactive oxygen species (hydrogen peroxide and superoxide radical), and the binding of ligands specific for albumin, namely fatty acids, and several drugs were evaluated. The results indicate that the labeling of albumin at its free cysteine residue (Cys-34) using 5-MSL may successfully be used for the detection of conformational changes, even in the case of the subtle alterations induced by ligand binding. © 2017, European Biophysical Societies' Association.
Glumac, Miodrag; Pejin, Boris; Karaman, Maja; Mojović, Miloš; Matavulj, Milan
Lignicolous fungi hydrodistilled extracts may represent a promising source of natural phenolics Journal Article
In: Natural Product Research, vol. 31, no. 1, pp. 104 – 107, 2017.
Abstract | Links | BibTeX | Tags:
@article{Glumac2017104,
title = {Lignicolous fungi hydrodistilled extracts may represent a promising source of natural phenolics},
author = {Miodrag Glumac and Boris Pejin and Maja Karaman and Miloš Mojović and Milan Matavulj},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84980011680&doi=10.1080%2f14786419.2016.1212036&partnerID=40&md5=6cbb10c2459c65358d608bae7fa2fe23},
doi = {10.1080/14786419.2016.1212036},
year = {2017},
date = {2017-01-01},
journal = {Natural Product Research},
volume = {31},
number = {1},
pages = {104 – 107},
abstract = {In vitro evaluation of total phenolic contents and antiradical activities of the lignicolous fungi Fomes fomentarius and Schizophyllum commune hydrodistilled extracts was the subject of this study. This preliminary screening included four free radical species evaluated by UV–vis (DPPH•, ABTS•and•NO) and EPR (Asc•), respectively. According to the experimental data obtained, both F. fomentarius and S. commune hydrodistilled extracts may be considered as promising sources of phenolic natural products (157 and 138 mg GAE/g d.e., respectively) and other bioactives showing good anti-DPPH (1.31 μg/mL) and anti-Asc (70.40%) radical activities, respectively, at in vitro conditions. © 2016 Informa UK Limited, trading as Taylor & Francis Group.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In vitro evaluation of total phenolic contents and antiradical activities of the lignicolous fungi Fomes fomentarius and Schizophyllum commune hydrodistilled extracts was the subject of this study. This preliminary screening included four free radical species evaluated by UV–vis (DPPH•, ABTS•and•NO) and EPR (Asc•), respectively. According to the experimental data obtained, both F. fomentarius and S. commune hydrodistilled extracts may be considered as promising sources of phenolic natural products (157 and 138 mg GAE/g d.e., respectively) and other bioactives showing good anti-DPPH (1.31 μg/mL) and anti-Asc (70.40%) radical activities, respectively, at in vitro conditions. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
Tošović, Jelena; Marković, Svetlana; Marković, Jasmina M. Dimitrić; Mojović, Miloš; Milenković, Dejan
Antioxidative mechanisms in chlorogenic acid Journal Article
In: Food Chemistry, vol. 237, pp. 390 – 398, 2017.
Abstract | Links | BibTeX | Tags:
@article{Tošović2017390,
title = {Antioxidative mechanisms in chlorogenic acid},
author = {Jelena Tošović and Svetlana Marković and Jasmina M. Dimitrić Marković and Miloš Mojović and Dejan Milenković},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019975230&doi=10.1016%2fj.foodchem.2017.05.080&partnerID=40&md5=64821c5eb6361bda6c568798811ff907},
doi = {10.1016/j.foodchem.2017.05.080},
year = {2017},
date = {2017-01-01},
journal = {Food Chemistry},
volume = {237},
pages = {390 – 398},
abstract = {Although chlorogenic acid (5CQA) is an important ingredient of various foods and beverages, mechanisms of its antioxidative action have not been fully clarified. Besides electron spin resonance experiment, this study includes thermodynamic and mechanistic investigations of the hydrogen atom transfer (HAT), radical adduct formation (RAF), sequential proton loss electron transfer (SPLET), and single electron transfer – proton transfer (SET-PT) mechanisms of 5CQA in benzene, ethanol, and water solutions. The calculations were performed using the M06-2X/6-311++G(d,p) level of theory and CPCM solvation model. It was found that SET-PT is not a plausible antioxidative mechanism of 5CQA. RAF pathways are faster, but HAT yields thermodynamically more stable radical products, indicating that in acidic and neutral media 5CQA can take either HAT or RAF pathways. In basic environment (e.g. at physiological pH) SPLET is the likely antioxidative mechanism of 5CQA with extremely high rate. © 2017 Elsevier Ltd},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Although chlorogenic acid (5CQA) is an important ingredient of various foods and beverages, mechanisms of its antioxidative action have not been fully clarified. Besides electron spin resonance experiment, this study includes thermodynamic and mechanistic investigations of the hydrogen atom transfer (HAT), radical adduct formation (RAF), sequential proton loss electron transfer (SPLET), and single electron transfer – proton transfer (SET-PT) mechanisms of 5CQA in benzene, ethanol, and water solutions. The calculations were performed using the M06-2X/6-311++G(d,p) level of theory and CPCM solvation model. It was found that SET-PT is not a plausible antioxidative mechanism of 5CQA. RAF pathways are faster, but HAT yields thermodynamically more stable radical products, indicating that in acidic and neutral media 5CQA can take either HAT or RAF pathways. In basic environment (e.g. at physiological pH) SPLET is the likely antioxidative mechanism of 5CQA with extremely high rate. © 2017 Elsevier Ltd