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2021
Vesković, A.; Nakarada, Đ.; Pavićević, A.; Prokić, B.; Perović, M.; Kanazir, S.; Popović-Bijelić, A.; Mojović, M.
In vivo/ex vivo EPR investigation of the brain redox status and blood-brain barrier integrity in the 5xFAD mouse model of Alzheimer's disease Journal Article
In: Current Alzheimer Research, vol. 18, no. 1, pp. 25-34, 2021, (cited By 1).
Abstract | Links | BibTeX | Tags:
@article{Vesković202125,
title = {In vivo/ex vivo EPR investigation of the brain redox status and blood-brain barrier integrity in the 5xFAD mouse model of Alzheimer's disease},
author = {A. Vesković and Đ. Nakarada and A. Pavićević and B. Prokić and M. Perović and S. Kanazir and A. Popović-Bijelić and M. Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104293047&doi=10.2174%2f1567205018666210324121156&partnerID=40&md5=49c60056dd19f58d7aebc09a3518eae1},
doi = {10.2174/1567205018666210324121156},
year = {2021},
date = {2021-01-01},
journal = {Current Alzheimer Research},
volume = {18},
number = {1},
pages = {25-34},
publisher = {Bentham Science Publishers},
abstract = {Background: Alzheimer’s disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the research focus from Aβ to other pathological features of the disease. Objective: The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. Methods: In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibiting different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectroscopy was employed to investigate the brain metal content. Results: This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-mediated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. Conclusion: The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neuronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spectroscopy application in preclinical studies of neurodegenerative diseases. © 2021 Bentham Science Publishers.},
note = {cited By 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background: Alzheimer’s disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the research focus from Aβ to other pathological features of the disease. Objective: The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. Methods: In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibiting different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectroscopy was employed to investigate the brain metal content. Results: This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-mediated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. Conclusion: The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neuronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spectroscopy application in preclinical studies of neurodegenerative diseases. © 2021 Bentham Science Publishers.
Grippo, V.; Mojovic, M.; Pavicevic, A.; Kabelac, M.; Hubatka, F.; Turanek, J.; Zatloukalova, M.; Freeman, B. A.; Vacek, J.
Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes Journal Article
In: Redox Biology, vol. 38, 2021, (cited By 13).
Abstract | Links | BibTeX | Tags:
@article{Grippo2021,
title = {Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes},
author = {V. Grippo and M. Mojovic and A. Pavicevic and M. Kabelac and F. Hubatka and J. Turanek and M. Zatloukalova and B. A. Freeman and J. Vacek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096158093&doi=10.1016%2fj.redox.2020.101756&partnerID=40&md5=2da8f31c4fb805691910ec16078ae42b},
doi = {10.1016/j.redox.2020.101756},
year = {2021},
date = {2021-01-01},
journal = {Redox Biology},
volume = {38},
publisher = {Elsevier B.V.},
abstract = {Fatty acid nitroalkenes (NO2-FA) are endogenously-generated products of the reaction of metabolic and inflammatory-derived nitrogen dioxide (.NO2) with unsaturated fatty acids. These species mediate signaling actions and induce adaptive responses in preclinical models of inflammatory and metabolic diseases. The nitroalkene substituent possesses an electrophilic nature, resulting in rapid and reversible reactions with biological nucleophiles such as cysteine, thus supporting post-translational modifications (PTM) of proteins having susceptible nucleophilic centers. These reactions contribute to enzyme regulation, modulation of inflammation and cell proliferation and the regulation of gene expression responses. Herein, focus is placed on the reduction-oxidation (redox) characteristics and stability of specific NO2-FA regioisomers having biological and clinical relevance; nitro-oleic acid (NO2-OA), bis-allylic nitro-linoleic acid (NO2-LA) and the conjugated diene-containing nitro-conjugated linoleic acid (NO2-cLA). Cyclic and alternating-current voltammetry and chronopotentiometry were used to the study of reduction potentials of these NO2-FA. R–NO2 reduction was observed around −0.8 V (vs. Ag/AgCl/3 M KCl) and is related to relative NO2-FA electrophilicity. This reduction process could be utilized for the evaluation of NO2-FA stability in aqueous milieu, shown herein to be pH dependent. In addition, electron paramagnetic resonance (EPR) spectroscopy was used to define the stability of the nitroalkene moiety under aqueous conditions, specifically under conditions where nitric oxide (.NO) release could be detected. The experimental data were supported by density functional theory calculations using 6–311++G (d,p) basis set and B3LYP functional. Based on experimental and computational approaches, the relative electrophilicities of these NO2-FA are NO2-cLA >> NO2-LA > NO2-OA. Micellarization and vesiculation largely define these biophysical characteristics in aqueous, nucleophile-free conditions. At concentrations below the critical micellar concentration (CMC), monomeric NO2-FA predominate, while at greater concentrations a micellar phase consisting of self-assembled lipid structures predominates. The CMC, determined by dynamic light scattering in 0.1 M phosphate buffer (pH 7.4) at 25 °C, was 6.9 (NO2-LA) 10.6 (NO2-OA) and 42.3 μM (NO2-cLA), respectively. In aggregate, this study provides new insight into the biophysical properties of NO2-FA that are important for better understanding the cell signaling and pharmacological potential of this class of mediators. © 2020 The Authors},
note = {cited By 13},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Fatty acid nitroalkenes (NO2-FA) are endogenously-generated products of the reaction of metabolic and inflammatory-derived nitrogen dioxide (.NO2) with unsaturated fatty acids. These species mediate signaling actions and induce adaptive responses in preclinical models of inflammatory and metabolic diseases. The nitroalkene substituent possesses an electrophilic nature, resulting in rapid and reversible reactions with biological nucleophiles such as cysteine, thus supporting post-translational modifications (PTM) of proteins having susceptible nucleophilic centers. These reactions contribute to enzyme regulation, modulation of inflammation and cell proliferation and the regulation of gene expression responses. Herein, focus is placed on the reduction-oxidation (redox) characteristics and stability of specific NO2-FA regioisomers having biological and clinical relevance; nitro-oleic acid (NO2-OA), bis-allylic nitro-linoleic acid (NO2-LA) and the conjugated diene-containing nitro-conjugated linoleic acid (NO2-cLA). Cyclic and alternating-current voltammetry and chronopotentiometry were used to the study of reduction potentials of these NO2-FA. R–NO2 reduction was observed around −0.8 V (vs. Ag/AgCl/3 M KCl) and is related to relative NO2-FA electrophilicity. This reduction process could be utilized for the evaluation of NO2-FA stability in aqueous milieu, shown herein to be pH dependent. In addition, electron paramagnetic resonance (EPR) spectroscopy was used to define the stability of the nitroalkene moiety under aqueous conditions, specifically under conditions where nitric oxide (.NO) release could be detected. The experimental data were supported by density functional theory calculations using 6–311++G (d,p) basis set and B3LYP functional. Based on experimental and computational approaches, the relative electrophilicities of these NO2-FA are NO2-cLA >> NO2-LA > NO2-OA. Micellarization and vesiculation largely define these biophysical characteristics in aqueous, nucleophile-free conditions. At concentrations below the critical micellar concentration (CMC), monomeric NO2-FA predominate, while at greater concentrations a micellar phase consisting of self-assembled lipid structures predominates. The CMC, determined by dynamic light scattering in 0.1 M phosphate buffer (pH 7.4) at 25 °C, was 6.9 (NO2-LA) 10.6 (NO2-OA) and 42.3 μM (NO2-cLA), respectively. In aggregate, this study provides new insight into the biophysical properties of NO2-FA that are important for better understanding the cell signaling and pharmacological potential of this class of mediators. © 2020 The Authors
Mojović, Z.; Petrović, S.; Mojović, M.; Pavlović, S.; Rožić, L.
Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection Journal Article
In: Journal of Physics and Chemistry of Solids, vol. 148, 2021, (cited By 4).
Abstract | Links | BibTeX | Tags:
@article{Mojović2021,
title = {Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection},
author = {Z. Mojović and S. Petrović and M. Mojović and S. Pavlović and L. Rožić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089660184&doi=10.1016%2fj.jpcs.2020.109649&partnerID=40&md5=2178ca9e6a6a97bf29af47c7530f08d5},
doi = {10.1016/j.jpcs.2020.109649},
year = {2021},
date = {2021-01-01},
journal = {Journal of Physics and Chemistry of Solids},
volume = {148},
publisher = {Elsevier Ltd},
abstract = {In this paper, the relationship of structure, type of oxygen defects, valence states of Cr and Ru, and electrochemical properties of La0.7Sr0.3Cr1-XRuXO3 (0 < x < 0.1) perovskite-type oxides were studied. The samples were synthesized by a conventional solid-state reaction method and characterized by powder X-ray diffraction, X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and electron paramagnetic resonance spectroscopy. The electrochemical performance of these samples was evaluated towards the detection of 4-nitrophenol and strongly depends on the concentration of ruthenium incorporated in the crystal lattice. A new La0.7Sr0.3Cr0.925Ru0.075O3 electrocatalyst with a wide linear concentration range (25–5000 mM) and a detection limit of 8 mM was obtained. These results provide an opportunity for its application in the analysis of real water samples. © 2020 Elsevier Ltd},
note = {cited By 4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this paper, the relationship of structure, type of oxygen defects, valence states of Cr and Ru, and electrochemical properties of La0.7Sr0.3Cr1-XRuXO3 (0 < x < 0.1) perovskite-type oxides were studied. The samples were synthesized by a conventional solid-state reaction method and characterized by powder X-ray diffraction, X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and electron paramagnetic resonance spectroscopy. The electrochemical performance of these samples was evaluated towards the detection of 4-nitrophenol and strongly depends on the concentration of ruthenium incorporated in the crystal lattice. A new La0.7Sr0.3Cr0.925Ru0.075O3 electrocatalyst with a wide linear concentration range (25–5000 mM) and a detection limit of 8 mM was obtained. These results provide an opportunity for its application in the analysis of real water samples. © 2020 Elsevier Ltd
Mojović, Zorica; Petrović, Srdjan; Mojović, Miloš; Pavlović, Stefan; Rožić, Ljiljana
Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection Journal Article
In: Journal of Physics and Chemistry of Solids, vol. 148, 2021, (Cited by: 5).
Abstract | Links | BibTeX | Tags:
@article{Mojović2021b,
title = {Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection},
author = {Zorica Mojović and Srdjan Petrović and Miloš Mojović and Stefan Pavlović and Ljiljana Rožić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089660184&doi=10.1016%2fj.jpcs.2020.109649&partnerID=40&md5=2178ca9e6a6a97bf29af47c7530f08d5},
doi = {10.1016/j.jpcs.2020.109649},
year = {2021},
date = {2021-01-01},
journal = {Journal of Physics and Chemistry of Solids},
volume = {148},
abstract = {In this paper, the relationship of structure, type of oxygen defects, valence states of Cr and Ru, and electrochemical properties of La0.7Sr0.3Cr1-XRuXO3 (0 < x < 0.1) perovskite-type oxides were studied. The samples were synthesized by a conventional solid-state reaction method and characterized by powder X-ray diffraction, X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and electron paramagnetic resonance spectroscopy. The electrochemical performance of these samples was evaluated towards the detection of 4-nitrophenol and strongly depends on the concentration of ruthenium incorporated in the crystal lattice. A new La0.7Sr0.3Cr0.925Ru0.075O3 electrocatalyst with a wide linear concentration range (25–5000 mM) and a detection limit of 8 mM was obtained. These results provide an opportunity for its application in the analysis of real water samples. © 2020 Elsevier Ltd},
note = {Cited by: 5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this paper, the relationship of structure, type of oxygen defects, valence states of Cr and Ru, and electrochemical properties of La0.7Sr0.3Cr1-XRuXO3 (0 < x < 0.1) perovskite-type oxides were studied. The samples were synthesized by a conventional solid-state reaction method and characterized by powder X-ray diffraction, X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and electron paramagnetic resonance spectroscopy. The electrochemical performance of these samples was evaluated towards the detection of 4-nitrophenol and strongly depends on the concentration of ruthenium incorporated in the crystal lattice. A new La0.7Sr0.3Cr0.925Ru0.075O3 electrocatalyst with a wide linear concentration range (25–5000 mM) and a detection limit of 8 mM was obtained. These results provide an opportunity for its application in the analysis of real water samples. © 2020 Elsevier Ltd
Grippo, Valentina; Mojovic, Milos; Pavicevic, Aleksandra; Kabelac, Martin; Hubatka, Frantisek; Turanek, Jaroslav; Zatloukalova, Martina; Freeman, Bruce A.; Vacek, Jan
Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes Journal Article
In: Redox Biology, vol. 38, 2021, (Cited by: 15; All Open Access, Gold Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Grippo2021b,
title = {Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes},
author = {Valentina Grippo and Milos Mojovic and Aleksandra Pavicevic and Martin Kabelac and Frantisek Hubatka and Jaroslav Turanek and Martina Zatloukalova and Bruce A. Freeman and Jan Vacek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096158093&doi=10.1016%2fj.redox.2020.101756&partnerID=40&md5=2da8f31c4fb805691910ec16078ae42b},
doi = {10.1016/j.redox.2020.101756},
year = {2021},
date = {2021-01-01},
journal = {Redox Biology},
volume = {38},
abstract = {Fatty acid nitroalkenes (NO2-FA) are endogenously-generated products of the reaction of metabolic and inflammatory-derived nitrogen dioxide (.NO2) with unsaturated fatty acids. These species mediate signaling actions and induce adaptive responses in preclinical models of inflammatory and metabolic diseases. The nitroalkene substituent possesses an electrophilic nature, resulting in rapid and reversible reactions with biological nucleophiles such as cysteine, thus supporting post-translational modifications (PTM) of proteins having susceptible nucleophilic centers. These reactions contribute to enzyme regulation, modulation of inflammation and cell proliferation and the regulation of gene expression responses. Herein, focus is placed on the reduction-oxidation (redox) characteristics and stability of specific NO2-FA regioisomers having biological and clinical relevance; nitro-oleic acid (NO2-OA), bis-allylic nitro-linoleic acid (NO2-LA) and the conjugated diene-containing nitro-conjugated linoleic acid (NO2-cLA). Cyclic and alternating-current voltammetry and chronopotentiometry were used to the study of reduction potentials of these NO2-FA. R–NO2 reduction was observed around −0.8 V (vs. Ag/AgCl/3 M KCl) and is related to relative NO2-FA electrophilicity. This reduction process could be utilized for the evaluation of NO2-FA stability in aqueous milieu, shown herein to be pH dependent. In addition, electron paramagnetic resonance (EPR) spectroscopy was used to define the stability of the nitroalkene moiety under aqueous conditions, specifically under conditions where nitric oxide (.NO) release could be detected. The experimental data were supported by density functional theory calculations using 6–311++G (d,p) basis set and B3LYP functional. Based on experimental and computational approaches, the relative electrophilicities of these NO2-FA are NO2-cLA >> NO2-LA > NO2-OA. Micellarization and vesiculation largely define these biophysical characteristics in aqueous, nucleophile-free conditions. At concentrations below the critical micellar concentration (CMC), monomeric NO2-FA predominate, while at greater concentrations a micellar phase consisting of self-assembled lipid structures predominates. The CMC, determined by dynamic light scattering in 0.1 M phosphate buffer (pH 7.4) at 25 °C, was 6.9 (NO2-LA) 10.6 (NO2-OA) and 42.3 μM (NO2-cLA), respectively. In aggregate, this study provides new insight into the biophysical properties of NO2-FA that are important for better understanding the cell signaling and pharmacological potential of this class of mediators. © 2020 The Authors},
note = {Cited by: 15; All Open Access, Gold Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Fatty acid nitroalkenes (NO2-FA) are endogenously-generated products of the reaction of metabolic and inflammatory-derived nitrogen dioxide (.NO2) with unsaturated fatty acids. These species mediate signaling actions and induce adaptive responses in preclinical models of inflammatory and metabolic diseases. The nitroalkene substituent possesses an electrophilic nature, resulting in rapid and reversible reactions with biological nucleophiles such as cysteine, thus supporting post-translational modifications (PTM) of proteins having susceptible nucleophilic centers. These reactions contribute to enzyme regulation, modulation of inflammation and cell proliferation and the regulation of gene expression responses. Herein, focus is placed on the reduction-oxidation (redox) characteristics and stability of specific NO2-FA regioisomers having biological and clinical relevance; nitro-oleic acid (NO2-OA), bis-allylic nitro-linoleic acid (NO2-LA) and the conjugated diene-containing nitro-conjugated linoleic acid (NO2-cLA). Cyclic and alternating-current voltammetry and chronopotentiometry were used to the study of reduction potentials of these NO2-FA. R–NO2 reduction was observed around −0.8 V (vs. Ag/AgCl/3 M KCl) and is related to relative NO2-FA electrophilicity. This reduction process could be utilized for the evaluation of NO2-FA stability in aqueous milieu, shown herein to be pH dependent. In addition, electron paramagnetic resonance (EPR) spectroscopy was used to define the stability of the nitroalkene moiety under aqueous conditions, specifically under conditions where nitric oxide (.NO) release could be detected. The experimental data were supported by density functional theory calculations using 6–311++G (d,p) basis set and B3LYP functional. Based on experimental and computational approaches, the relative electrophilicities of these NO2-FA are NO2-cLA >> NO2-LA > NO2-OA. Micellarization and vesiculation largely define these biophysical characteristics in aqueous, nucleophile-free conditions. At concentrations below the critical micellar concentration (CMC), monomeric NO2-FA predominate, while at greater concentrations a micellar phase consisting of self-assembled lipid structures predominates. The CMC, determined by dynamic light scattering in 0.1 M phosphate buffer (pH 7.4) at 25 °C, was 6.9 (NO2-LA) 10.6 (NO2-OA) and 42.3 μM (NO2-cLA), respectively. In aggregate, this study provides new insight into the biophysical properties of NO2-FA that are important for better understanding the cell signaling and pharmacological potential of this class of mediators. © 2020 The Authors
2020
Mišurović, J.; Mojović, M.; Marjanović, B.; Vulić, P.; Ćirić-Marjanović, G.
Magnetite nanoparticles-catalyzed synthesis of conductive poly(p-aminodiphenylamine) Journal Article
In: Synthetic Metals, vol. 269, 2020, (cited By 1).
Abstract | Links | BibTeX | Tags:
@article{Mišurović2020,
title = {Magnetite nanoparticles-catalyzed synthesis of conductive poly(p-aminodiphenylamine)},
author = {J. Mišurović and M. Mojović and B. Marjanović and P. Vulić and G. Ćirić-Marjanović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091590368&doi=10.1016%2fj.synthmet.2020.116577&partnerID=40&md5=9d58d17505031d1fc2956c8449632aa1},
doi = {10.1016/j.synthmet.2020.116577},
year = {2020},
date = {2020-01-01},
journal = {Synthetic Metals},
volume = {269},
publisher = {Elsevier Ltd},
abstract = {Para-aminodiphenylamine (p-ADPA) was successfully polymerized by simple, economical and environmentally improved procedure. Magnetite nanoparticles (Fe3O4 NPs) were found to successfully catalyze the oxidation of p-ADPA in the presence of hydrogen peroxide (H2O2), an eco-friendly and clean oxidant, with a very small amount of ammonium peroxydisulfate (APS) as an initiator. In this manner, the amount of by-products occurring during the reaction is drastically reduced. The oxidation of p-ADPA proceeded also in the absence of Fe3O4 NPs, but far more slowly than the catalyzed process. The influence of the mass ratio Fe3O4NPs/p-ADPA on the properties of products was systematically studied by different characterization techniques. Spectroscopic measurements (UV-Vis-NIR, FTIR, Raman, EPR) indicate that the polymerization products are polyaniline emeraldine salt-like polymers and their measured electrical conductivity is one of the highest values reported in the literature for p-ADPA oligomerization/polymerization products (1.8∙10-2 S cm-1). X-ray powder diffraction (XRPD) measurements revealed highly crystalline structure of Poly(p-ADPA) samples synthesized with Fe3O4 NPs, while scanning electron microscopy (SEM) showed layered and prismatic-shaped particles as prevailing in their morphology. © 2020 Elsevier B.V.},
note = {cited By 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Para-aminodiphenylamine (p-ADPA) was successfully polymerized by simple, economical and environmentally improved procedure. Magnetite nanoparticles (Fe3O4 NPs) were found to successfully catalyze the oxidation of p-ADPA in the presence of hydrogen peroxide (H2O2), an eco-friendly and clean oxidant, with a very small amount of ammonium peroxydisulfate (APS) as an initiator. In this manner, the amount of by-products occurring during the reaction is drastically reduced. The oxidation of p-ADPA proceeded also in the absence of Fe3O4 NPs, but far more slowly than the catalyzed process. The influence of the mass ratio Fe3O4NPs/p-ADPA on the properties of products was systematically studied by different characterization techniques. Spectroscopic measurements (UV-Vis-NIR, FTIR, Raman, EPR) indicate that the polymerization products are polyaniline emeraldine salt-like polymers and their measured electrical conductivity is one of the highest values reported in the literature for p-ADPA oligomerization/polymerization products (1.8∙10-2 S cm-1). X-ray powder diffraction (XRPD) measurements revealed highly crystalline structure of Poly(p-ADPA) samples synthesized with Fe3O4 NPs, while scanning electron microscopy (SEM) showed layered and prismatic-shaped particles as prevailing in their morphology. © 2020 Elsevier B.V.
Rajić, V.; Simatović, I. Stojković; Veselinović, L.; Čavor, J. B.; Novaković, M.; Popović, M.; Škapin, S. D.; Mojović, M.; Stojadinović, S.; Rac, V.; Častvan, I. J.; Marković, S.
Bifunctional catalytic activity of Zn1-: XFexO toward the OER/ORR: Seeking an optimal stoichiometry Journal Article
In: Physical Chemistry Chemical Physics, vol. 22, no. 38, pp. 22078-22095, 2020, (cited By 4).
Abstract | Links | BibTeX | Tags:
@article{Rajić202022078,
title = {Bifunctional catalytic activity of Zn1-: XFexO toward the OER/ORR: Seeking an optimal stoichiometry},
author = {V. Rajić and I. Stojković Simatović and L. Veselinović and J. B. Čavor and M. Novaković and M. Popović and S. D. Škapin and M. Mojović and S. Stojadinović and V. Rac and I. J. Častvan and S. Marković},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092681262&doi=10.1039%2fd0cp03377d&partnerID=40&md5=40bcf6262611a1231b60b7ecb5910e44},
doi = {10.1039/d0cp03377d},
year = {2020},
date = {2020-01-01},
journal = {Physical Chemistry Chemical Physics},
volume = {22},
number = {38},
pages = {22078-22095},
publisher = {Royal Society of Chemistry},
abstract = {Eco-friendly and rapid microwave processing of a precipitate was used to produce Fe-doped zinc oxide (Zn1-xFexO},
note = {cited By 4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Eco-friendly and rapid microwave processing of a precipitate was used to produce Fe-doped zinc oxide (Zn1-xFexO
Nakarada, Đ.; Pejin, B.; Tommonaro, G.; Mojović, M.
In: Journal of Liposome Research, vol. 30, no. 3, pp. 218-226, 2020, (cited By 7).
Abstract | Links | BibTeX | Tags:
@article{Nakarada2020218,
title = {Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol},
author = {Đ. Nakarada and B. Pejin and G. Tommonaro and M. Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067698469&doi=10.1080%2f08982104.2019.1625378&partnerID=40&md5=99e8b9d2e73b4f03bac1888b0acd0d59},
doi = {10.1080/08982104.2019.1625378},
year = {2020},
date = {2020-01-01},
journal = {Journal of Liposome Research},
volume = {30},
number = {3},
pages = {218-226},
publisher = {Taylor and Francis Ltd.},
abstract = {The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards •OH, O2•− and NO• radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol’s activity towards •OH, O2•−, NO• and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential. © 2019 Informa UK Limited, trading as Taylor & Francis Group.},
note = {cited By 7},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards •OH, O2•− and NO• radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol’s activity towards •OH, O2•−, NO• and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential. © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Matijević, M.; Nakarada, Đ.; Liang, X.; Korićanac, L.; Rajsiglova, L.; Vannucci, L.; Nešić, M.; Vranješ, M.; Mojović, M.; Mi, L.; Estrela-Lopis, I.; Böttner, J.; Šaponjić, Z.; Petković, M.; Stepić, M.
Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer Journal Article
In: Journal of Nanoparticle Research, vol. 22, no. 7, 2020, (cited By 5).
Abstract | Links | BibTeX | Tags:
@article{Matijević2020,
title = {Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer},
author = {M. Matijević and Đ. Nakarada and X. Liang and L. Korićanac and L. Rajsiglova and L. Vannucci and M. Nešić and M. Vranješ and M. Mojović and L. Mi and I. Estrela-Lopis and J. Böttner and Z. Šaponjić and M. Petković and M. Stepić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087054250&doi=10.1007%2fs11051-020-04899-3&partnerID=40&md5=9019709ed3fedee914b332555d4534ce},
doi = {10.1007/s11051-020-04899-3},
year = {2020},
date = {2020-01-01},
journal = {Journal of Nanoparticle Research},
volume = {22},
number = {7},
publisher = {Springer},
abstract = {TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study. © 2020, Springer Nature B.V.},
note = {cited By 5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study. © 2020, Springer Nature B.V.
Vujacic-Mirski, K.; Bruns, K.; Kalinovic, S.; Oelze, M.; Kröller-Schön, S.; Steven, S.; Mojovic, M.; Korac, B.; Münzel, T.; Daiber, A.
In: Antioxidants, vol. 9, no. 5, 2020, (cited By 5).
Abstract | Links | BibTeX | Tags:
@article{Vujacic-Mirski2020,
title = {Development of an analytical assay for electrochemical detection and quantification of protein-bound 3-nitrotyrosine in biological samples and comparison with classical, antibody-based methods},
author = {K. Vujacic-Mirski and K. Bruns and S. Kalinovic and M. Oelze and S. Kröller-Schön and S. Steven and M. Mojovic and B. Korac and T. Münzel and A. Daiber},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084254228&doi=10.3390%2fantiox9050388&partnerID=40&md5=9144982ce1a9eea136a9663ecf80df32},
doi = {10.3390/antiox9050388},
year = {2020},
date = {2020-01-01},
journal = {Antioxidants},
volume = {9},
number = {5},
publisher = {MDPI},
abstract = {Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantified by electrochemical detection (ECD, CoulArray) and compared to classical methods, namely enzyme-linked immunosorbent assay (ELISA) and dot blot analysis using specific 3-NT antibodies. Calibration curves for authentic 3-NT (detection limit 10 nM) and a concentration-response pattern for 3-NT obtained from digested nitrated bovine serum albumin (BSA) were highly linear over a wide 3-NT concentration range. Also, ex vivo nitration of protein from heart, isolated mitochondria, and serum/plasma could be quantified using the HPLC/ECD method and was confirmed by LC-MS/MS. Of note, nitro-oxidative damage of mitochondria results in increased superoxide (O2 •–) formation rates (measured by dihydroethidium-based HPLC assay), pointing to a self-amplification mechanism of oxidative stress. Based on our ex vivo data, the CoulArray quantification method for 3-NT seems to have some advantages regarding sensitivity and selectivity. Establishing a reliable automated HPLC assay for the routine quantification of 3-NT in biological samples of cell culture, of animal and human origin seems to be more sophisticated than expected. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {cited By 5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantified by electrochemical detection (ECD, CoulArray) and compared to classical methods, namely enzyme-linked immunosorbent assay (ELISA) and dot blot analysis using specific 3-NT antibodies. Calibration curves for authentic 3-NT (detection limit 10 nM) and a concentration-response pattern for 3-NT obtained from digested nitrated bovine serum albumin (BSA) were highly linear over a wide 3-NT concentration range. Also, ex vivo nitration of protein from heart, isolated mitochondria, and serum/plasma could be quantified using the HPLC/ECD method and was confirmed by LC-MS/MS. Of note, nitro-oxidative damage of mitochondria results in increased superoxide (O2 •–) formation rates (measured by dihydroethidium-based HPLC assay), pointing to a self-amplification mechanism of oxidative stress. Based on our ex vivo data, the CoulArray quantification method for 3-NT seems to have some advantages regarding sensitivity and selectivity. Establishing a reliable automated HPLC assay for the routine quantification of 3-NT in biological samples of cell culture, of animal and human origin seems to be more sophisticated than expected. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Carević, M. V.; Savić, T. D.; Abazović, N. D.; Mojović, M. D.; Novaković, T. B.; Čomor, M. I.
Effect of Fe3+ ion doping on photocatalytic ability of nanozirconia ceramic to degrade 2, 4, 6- trichlorophenol Journal Article
In: Ceramics International, vol. 46, no. 5, pp. 6820-6827, 2020, (cited By 6).
Abstract | Links | BibTeX | Tags:
@article{Carević20206820,
title = {Effect of Fe3+ ion doping on photocatalytic ability of nanozirconia ceramic to degrade 2, 4, 6- trichlorophenol},
author = {M. V. Carević and T. D. Savić and N. D. Abazović and M. D. Mojović and T. B. Novaković and M. I. Čomor},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078751596&doi=10.1016%2fj.ceramint.2019.11.175&partnerID=40&md5=1d7e4c1e95c26cae0304d07dcdf02f98},
doi = {10.1016/j.ceramint.2019.11.175},
year = {2020},
date = {2020-01-01},
journal = {Ceramics International},
volume = {46},
number = {5},
pages = {6820-6827},
publisher = {Elsevier Ltd},
abstract = {Pure and a series of Fe3+ doped zirconia nanopowders were synthesized combining colloidal chemistry and solvothermal method from organometallic precursors in order to correlate doping and properties of zirconia matrix. After annealing of washed nanopowders at 600 °C, detailed characterization was performed using X-ray diffraction, UV/Vis absorption and luminescence, infrared and electron paramagnetic spectroscopy, transmission electron microscopy and BET measurements. Zirconia samples had mixed monoclinic and tetragonal crystalline phase; increasing Fe3+ ions concentration was followed by increasing of tetragonal phase share. In the sample with highest Fe3+ concentration, hematite can be detected. Also, UV/Vis spectrophotometry showed that Fe3+ doping lowers effective band gap of zirconia matrix from 4.5 eV (bulk value) to 2.1 eV for doped/nanocomposite samples. EPR measurements proved presence of dopant and showed that isolated Fe3+ ions in zirconia matrix exist in both crystalline phases; monoclinic and tetragonal (g ~ 4.8 and g ~ 4.27–4.2, respectively) surroundings. Zirconia samples were also successfully used as photocatalysts for photocatalytic degradation of 2, 4, 6, trichlorophenol. © 2019 Elsevier Ltd and Techna Group S.r.l.},
note = {cited By 6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pure and a series of Fe3+ doped zirconia nanopowders were synthesized combining colloidal chemistry and solvothermal method from organometallic precursors in order to correlate doping and properties of zirconia matrix. After annealing of washed nanopowders at 600 °C, detailed characterization was performed using X-ray diffraction, UV/Vis absorption and luminescence, infrared and electron paramagnetic spectroscopy, transmission electron microscopy and BET measurements. Zirconia samples had mixed monoclinic and tetragonal crystalline phase; increasing Fe3+ ions concentration was followed by increasing of tetragonal phase share. In the sample with highest Fe3+ concentration, hematite can be detected. Also, UV/Vis spectrophotometry showed that Fe3+ doping lowers effective band gap of zirconia matrix from 4.5 eV (bulk value) to 2.1 eV for doped/nanocomposite samples. EPR measurements proved presence of dopant and showed that isolated Fe3+ ions in zirconia matrix exist in both crystalline phases; monoclinic and tetragonal (g ~ 4.8 and g ~ 4.27–4.2, respectively) surroundings. Zirconia samples were also successfully used as photocatalysts for photocatalytic degradation of 2, 4, 6, trichlorophenol. © 2019 Elsevier Ltd and Techna Group S.r.l.
Dimić, D.; Nakarada, D.; Mojović, M.; Marković, J. D.
An experimental and theoretical study of the reactivity of selected catecholamines and their precursors towards ascorbyl radical Journal Article
In: Journal of the Serbian Society for Computational Mechanics, no. SpecialIssue, pp. 1-12, 2020, (cited By 2).
Abstract | Links | BibTeX | Tags:
@article{Dimić20201,
title = {An experimental and theoretical study of the reactivity of selected catecholamines and their precursors towards ascorbyl radical},
author = {D. Dimić and D. Nakarada and M. Mojović and J. D. Marković},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089268212&doi=10.24874%2fjsscm.2020.01.01&partnerID=40&md5=53a5048f1ed5fbd05fef983f7cc340fc},
doi = {10.24874/jsscm.2020.01.01},
year = {2020},
date = {2020-01-01},
journal = {Journal of the Serbian Society for Computational Mechanics},
number = {SpecialIssue},
pages = {1-12},
publisher = {Serbian Society of Computational Mechanics},
abstract = {Ascorbyl radical is often used as a biomarker of oxidative stress in human organism. It is a product of the antioxidant activity of ascorbic acid and it is not expected to react further with biologically important molecules. For the first time, the reactivity of catecholamines and their precursors was investigated theoretically and experimentally towards ascorbyl radical and the main structural parameters governing activity were analyzed. It was shown that catechol moiety plays an important role, which classifies norepinephrine and 3,4-dihydroxyphenylacetic acid as the most reactive when compared to homovanillic acid, vanillylmandelic acid, and octopamine. DFT methods have been employed to determine the most probable mechanism of the reaction. Based on the change in reaction enthalpy it was concluded that Hydrogen Atom Transfer (HAT) is a preferred mechanism both in water and pentyl ethanoate. The stabilization interactions in ascorbic acid, its anion and radical are carefully analyzed in order to understand the preferability of the mentioned mechanism. The stability of the ascorbyl radical is explained in detail. The results prove that ascorbyl radical is not just a product of antioxidant activity, but also a potentially harmful species in an organism. © 2020, Serbian Society of Computational Mechanics.},
note = {cited By 2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ascorbyl radical is often used as a biomarker of oxidative stress in human organism. It is a product of the antioxidant activity of ascorbic acid and it is not expected to react further with biologically important molecules. For the first time, the reactivity of catecholamines and their precursors was investigated theoretically and experimentally towards ascorbyl radical and the main structural parameters governing activity were analyzed. It was shown that catechol moiety plays an important role, which classifies norepinephrine and 3,4-dihydroxyphenylacetic acid as the most reactive when compared to homovanillic acid, vanillylmandelic acid, and octopamine. DFT methods have been employed to determine the most probable mechanism of the reaction. Based on the change in reaction enthalpy it was concluded that Hydrogen Atom Transfer (HAT) is a preferred mechanism both in water and pentyl ethanoate. The stabilization interactions in ascorbic acid, its anion and radical are carefully analyzed in order to understand the preferability of the mentioned mechanism. The stability of the ascorbyl radical is explained in detail. The results prove that ascorbyl radical is not just a product of antioxidant activity, but also a potentially harmful species in an organism. © 2020, Serbian Society of Computational Mechanics.
Zang, L.; Paven, M. -C. Morère-Le; Clochard, T.; Porcher, A.; Satour, P.; Mojović, M.; Vidović, M.; Limami, A. M.; Montrichard, F.
Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula Journal Article
In: Plant Physiology and Biochemistry, vol. 146, pp. 363-373, 2020, (cited By 12).
Abstract | Links | BibTeX | Tags:
@article{Zang2020363,
title = {Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula},
author = {L. Zang and M. -C. Morère-Le Paven and T. Clochard and A. Porcher and P. Satour and M. Mojović and M. Vidović and A. M. Limami and F. Montrichard},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075562315&doi=10.1016%2fj.plaphy.2019.11.006&partnerID=40&md5=8ce9220ea6bedb365cdc4b0f2655e7fa},
doi = {10.1016/j.plaphy.2019.11.006},
year = {2020},
date = {2020-01-01},
journal = {Plant Physiology and Biochemistry},
volume = {146},
pages = {363-373},
publisher = {Elsevier Masson SAS},
abstract = {In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O2 •− → H2O2 → •OH) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O2 •−, H2O2 and •OH in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O2 •− accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into •OH. These changes impair the accumulation of H2O2 and then the accumulation of •OH, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway. © 2019 Elsevier Masson SAS},
note = {cited By 12},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O2 •− → H2O2 → •OH) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O2 •−, H2O2 and •OH in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O2 •− accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into •OH. These changes impair the accumulation of H2O2 and then the accumulation of •OH, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway. © 2019 Elsevier Masson SAS
Matijević, Milica; Nakarada, Đura; Liang, Xinyue; Korićanac, Lela; Rajsiglova, Lenka; Vannucci, Luca; Nešić, Maja; Vranješ, Mila; Mojović, Miloš; Mi, Lan; Estrela-Lopis, Irina; Böttner, Julia; Šaponjić, Zoran; Petković, Marijana; Stepić, Milutin
Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer Journal Article
In: Journal of Nanoparticle Research, vol. 22, no. 7, 2020, (Cited by: 6).
Abstract | Links | BibTeX | Tags:
@article{Matijević2020b,
title = {Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer},
author = {Milica Matijević and Đura Nakarada and Xinyue Liang and Lela Korićanac and Lenka Rajsiglova and Luca Vannucci and Maja Nešić and Mila Vranješ and Miloš Mojović and Lan Mi and Irina Estrela-Lopis and Julia Böttner and Zoran Šaponjić and Marijana Petković and Milutin Stepić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087054250&doi=10.1007%2fs11051-020-04899-3&partnerID=40&md5=9019709ed3fedee914b332555d4534ce},
doi = {10.1007/s11051-020-04899-3},
year = {2020},
date = {2020-01-01},
journal = {Journal of Nanoparticle Research},
volume = {22},
number = {7},
abstract = {TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study. © 2020, Springer Nature B.V.},
note = {Cited by: 6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study. © 2020, Springer Nature B.V.
Nakarada, Đura; Pejin, Boris; Tommonaro, Giuseppina; Mojović, Miloš
In: Journal of Liposome Research, vol. 30, no. 3, pp. 218 – 226, 2020, (Cited by: 9).
Abstract | Links | BibTeX | Tags:
@article{Nakarada2020218b,
title = {Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol},
author = {Đura Nakarada and Boris Pejin and Giuseppina Tommonaro and Miloš Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067698469&doi=10.1080%2f08982104.2019.1625378&partnerID=40&md5=99e8b9d2e73b4f03bac1888b0acd0d59},
doi = {10.1080/08982104.2019.1625378},
year = {2020},
date = {2020-01-01},
journal = {Journal of Liposome Research},
volume = {30},
number = {3},
pages = {218 – 226},
abstract = {The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards •OH, O2•− and NO• radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol’s activity towards •OH, O2•−, NO• and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential. © 2019 Informa UK Limited, trading as Taylor & Francis Group.},
note = {Cited by: 9},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards •OH, O2•− and NO• radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol’s activity towards •OH, O2•−, NO• and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential. © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Dimić, Dušan; Nakarada, Dura; Mojović, Miloš; Marković, Jasmina Dimitrić
An experimental and theoretical study of the reactivity of selected catecholamines and their precursors towards ascorbyl radical Journal Article
In: Journal of the Serbian Society for Computational Mechanics, no. SpecialIssue, pp. 1 – 12, 2020, (Cited by: 2; All Open Access, Bronze Open Access).
Abstract | Links | BibTeX | Tags:
@article{Dimić20201b,
title = {An experimental and theoretical study of the reactivity of selected catecholamines and their precursors towards ascorbyl radical},
author = {Dušan Dimić and Dura Nakarada and Miloš Mojović and Jasmina Dimitrić Marković},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089268212&doi=10.24874%2fjsscm.2020.01.01&partnerID=40&md5=53a5048f1ed5fbd05fef983f7cc340fc},
doi = {10.24874/jsscm.2020.01.01},
year = {2020},
date = {2020-01-01},
journal = {Journal of the Serbian Society for Computational Mechanics},
number = {SpecialIssue},
pages = {1 – 12},
abstract = {Ascorbyl radical is often used as a biomarker of oxidative stress in human organism. It is a product of the antioxidant activity of ascorbic acid and it is not expected to react further with biologically important molecules. For the first time, the reactivity of catecholamines and their precursors was investigated theoretically and experimentally towards ascorbyl radical and the main structural parameters governing activity were analyzed. It was shown that catechol moiety plays an important role, which classifies norepinephrine and 3,4-dihydroxyphenylacetic acid as the most reactive when compared to homovanillic acid, vanillylmandelic acid, and octopamine. DFT methods have been employed to determine the most probable mechanism of the reaction. Based on the change in reaction enthalpy it was concluded that Hydrogen Atom Transfer (HAT) is a preferred mechanism both in water and pentyl ethanoate. The stabilization interactions in ascorbic acid, its anion and radical are carefully analyzed in order to understand the preferability of the mentioned mechanism. The stability of the ascorbyl radical is explained in detail. The results prove that ascorbyl radical is not just a product of antioxidant activity, but also a potentially harmful species in an organism. © 2020, Serbian Society of Computational Mechanics.},
note = {Cited by: 2; All Open Access, Bronze Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ascorbyl radical is often used as a biomarker of oxidative stress in human organism. It is a product of the antioxidant activity of ascorbic acid and it is not expected to react further with biologically important molecules. For the first time, the reactivity of catecholamines and their precursors was investigated theoretically and experimentally towards ascorbyl radical and the main structural parameters governing activity were analyzed. It was shown that catechol moiety plays an important role, which classifies norepinephrine and 3,4-dihydroxyphenylacetic acid as the most reactive when compared to homovanillic acid, vanillylmandelic acid, and octopamine. DFT methods have been employed to determine the most probable mechanism of the reaction. Based on the change in reaction enthalpy it was concluded that Hydrogen Atom Transfer (HAT) is a preferred mechanism both in water and pentyl ethanoate. The stabilization interactions in ascorbic acid, its anion and radical are carefully analyzed in order to understand the preferability of the mentioned mechanism. The stability of the ascorbyl radical is explained in detail. The results prove that ascorbyl radical is not just a product of antioxidant activity, but also a potentially harmful species in an organism. © 2020, Serbian Society of Computational Mechanics.
Mišurović, Jana; Mojović, Miloš; Marjanović, Budimir; Vulić, Predrag; Ćirić-Marjanović, Gordana
Magnetite nanoparticles-catalyzed synthesis of conductive poly(p-aminodiphenylamine) Journal Article
In: Synthetic Metals, vol. 269, 2020, (Cited by: 1).
Abstract | Links | BibTeX | Tags:
@article{Mišurović2020b,
title = {Magnetite nanoparticles-catalyzed synthesis of conductive poly(p-aminodiphenylamine)},
author = {Jana Mišurović and Miloš Mojović and Budimir Marjanović and Predrag Vulić and Gordana Ćirić-Marjanović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091590368&doi=10.1016%2fj.synthmet.2020.116577&partnerID=40&md5=9d58d17505031d1fc2956c8449632aa1},
doi = {10.1016/j.synthmet.2020.116577},
year = {2020},
date = {2020-01-01},
journal = {Synthetic Metals},
volume = {269},
abstract = {Para-aminodiphenylamine (p-ADPA) was successfully polymerized by simple, economical and environmentally improved procedure. Magnetite nanoparticles (Fe3O4 NPs) were found to successfully catalyze the oxidation of p-ADPA in the presence of hydrogen peroxide (H2O2), an eco-friendly and clean oxidant, with a very small amount of ammonium peroxydisulfate (APS) as an initiator. In this manner, the amount of by-products occurring during the reaction is drastically reduced. The oxidation of p-ADPA proceeded also in the absence of Fe3O4 NPs, but far more slowly than the catalyzed process. The influence of the mass ratio Fe3O4NPs/p-ADPA on the properties of products was systematically studied by different characterization techniques. Spectroscopic measurements (UV-Vis-NIR, FTIR, Raman, EPR) indicate that the polymerization products are polyaniline emeraldine salt-like polymers and their measured electrical conductivity is one of the highest values reported in the literature for p-ADPA oligomerization/polymerization products (1.8∙10-2 S cm-1). X-ray powder diffraction (XRPD) measurements revealed highly crystalline structure of Poly(p-ADPA) samples synthesized with Fe3O4 NPs, while scanning electron microscopy (SEM) showed layered and prismatic-shaped particles as prevailing in their morphology. © 2020 Elsevier B.V.},
note = {Cited by: 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Para-aminodiphenylamine (p-ADPA) was successfully polymerized by simple, economical and environmentally improved procedure. Magnetite nanoparticles (Fe3O4 NPs) were found to successfully catalyze the oxidation of p-ADPA in the presence of hydrogen peroxide (H2O2), an eco-friendly and clean oxidant, with a very small amount of ammonium peroxydisulfate (APS) as an initiator. In this manner, the amount of by-products occurring during the reaction is drastically reduced. The oxidation of p-ADPA proceeded also in the absence of Fe3O4 NPs, but far more slowly than the catalyzed process. The influence of the mass ratio Fe3O4NPs/p-ADPA on the properties of products was systematically studied by different characterization techniques. Spectroscopic measurements (UV-Vis-NIR, FTIR, Raman, EPR) indicate that the polymerization products are polyaniline emeraldine salt-like polymers and their measured electrical conductivity is one of the highest values reported in the literature for p-ADPA oligomerization/polymerization products (1.8∙10-2 S cm-1). X-ray powder diffraction (XRPD) measurements revealed highly crystalline structure of Poly(p-ADPA) samples synthesized with Fe3O4 NPs, while scanning electron microscopy (SEM) showed layered and prismatic-shaped particles as prevailing in their morphology. © 2020 Elsevier B.V.
Rajić, Vladimir; Simatović, Ivana Stojković; Veselinović, Ljiljana; Čavor, Jelena Belošević; Novaković, Mirjana; Popović, Maja; Škapin, Srečo Davor; Mojović, Miloš; Stojadinović, Stevan; Rac, Vladislav; Častvan, Ivona Janković; Marković, Smilja
Bifunctional catalytic activity of Zn1-: X Fex O toward the OER/ORR: Seeking an optimal stoichiometry Journal Article
In: Physical Chemistry Chemical Physics, vol. 22, no. 38, pp. 22078 – 22095, 2020, (Cited by: 8; All Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Rajić202022078b,
title = {Bifunctional catalytic activity of Zn1-: X Fex O toward the OER/ORR: Seeking an optimal stoichiometry},
author = {Vladimir Rajić and Ivana Stojković Simatović and Ljiljana Veselinović and Jelena Belošević Čavor and Mirjana Novaković and Maja Popović and Srečo Davor Škapin and Miloš Mojović and Stevan Stojadinović and Vladislav Rac and Ivona Janković Častvan and Smilja Marković},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092681262&doi=10.1039%2fd0cp03377d&partnerID=40&md5=40bcf6262611a1231b60b7ecb5910e44},
doi = {10.1039/d0cp03377d},
year = {2020},
date = {2020-01-01},
journal = {Physical Chemistry Chemical Physics},
volume = {22},
number = {38},
pages = {22078 – 22095},
abstract = {Eco-friendly and rapid microwave processing of a precipitate was used to produce Fe-doped zinc oxide (Zn1-xFexO},
note = {Cited by: 8; All Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Eco-friendly and rapid microwave processing of a precipitate was used to produce Fe-doped zinc oxide (Zn1-xFexO
Carević, Milica V.; Savić, Tatjana D.; Abazović, Nadica D.; Mojović, Miloš D.; Novaković, Tatjana B.; Čomor, Mirjana I.
Effect of Fe3+ ion doping on photocatalytic ability of nanozirconia ceramic to degrade 2, 4, 6- trichlorophenol Journal Article
In: Ceramics International, vol. 46, no. 5, pp. 6820 – 6827, 2020, (Cited by: 6; All Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Carević20206820b,
title = {Effect of Fe3+ ion doping on photocatalytic ability of nanozirconia ceramic to degrade 2, 4, 6- trichlorophenol},
author = {Milica V. Carević and Tatjana D. Savić and Nadica D. Abazović and Miloš D. Mojović and Tatjana B. Novaković and Mirjana I. Čomor},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078751596&doi=10.1016%2fj.ceramint.2019.11.175&partnerID=40&md5=1d7e4c1e95c26cae0304d07dcdf02f98},
doi = {10.1016/j.ceramint.2019.11.175},
year = {2020},
date = {2020-01-01},
journal = {Ceramics International},
volume = {46},
number = {5},
pages = {6820 – 6827},
abstract = {Pure and a series of Fe3+ doped zirconia nanopowders were synthesized combining colloidal chemistry and solvothermal method from organometallic precursors in order to correlate doping and properties of zirconia matrix. After annealing of washed nanopowders at 600 °C, detailed characterization was performed using X-ray diffraction, UV/Vis absorption and luminescence, infrared and electron paramagnetic spectroscopy, transmission electron microscopy and BET measurements. Zirconia samples had mixed monoclinic and tetragonal crystalline phase; increasing Fe3+ ions concentration was followed by increasing of tetragonal phase share. In the sample with highest Fe3+ concentration, hematite can be detected. Also, UV/Vis spectrophotometry showed that Fe3+ doping lowers effective band gap of zirconia matrix from 4.5 eV (bulk value) to 2.1 eV for doped/nanocomposite samples. EPR measurements proved presence of dopant and showed that isolated Fe3+ ions in zirconia matrix exist in both crystalline phases; monoclinic and tetragonal (g ~ 4.8 and g ~ 4.27–4.2, respectively) surroundings. Zirconia samples were also successfully used as photocatalysts for photocatalytic degradation of 2, 4, 6, trichlorophenol. © 2019 Elsevier Ltd and Techna Group S.r.l.},
note = {Cited by: 6; All Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pure and a series of Fe3+ doped zirconia nanopowders were synthesized combining colloidal chemistry and solvothermal method from organometallic precursors in order to correlate doping and properties of zirconia matrix. After annealing of washed nanopowders at 600 °C, detailed characterization was performed using X-ray diffraction, UV/Vis absorption and luminescence, infrared and electron paramagnetic spectroscopy, transmission electron microscopy and BET measurements. Zirconia samples had mixed monoclinic and tetragonal crystalline phase; increasing Fe3+ ions concentration was followed by increasing of tetragonal phase share. In the sample with highest Fe3+ concentration, hematite can be detected. Also, UV/Vis spectrophotometry showed that Fe3+ doping lowers effective band gap of zirconia matrix from 4.5 eV (bulk value) to 2.1 eV for doped/nanocomposite samples. EPR measurements proved presence of dopant and showed that isolated Fe3+ ions in zirconia matrix exist in both crystalline phases; monoclinic and tetragonal (g ~ 4.8 and g ~ 4.27–4.2, respectively) surroundings. Zirconia samples were also successfully used as photocatalysts for photocatalytic degradation of 2, 4, 6, trichlorophenol. © 2019 Elsevier Ltd and Techna Group S.r.l.
Vujacic-Mirski, Ksenija; Bruns, Kai; Kalinovic, Sanela; Oelze, Matthias; Kröller-Schön, Swenja; Steven, Sebastian; Mojovic, Milos; Korac, Bato; Münzel, Thomas; Daiber, Andreas
In: Antioxidants, vol. 9, no. 5, 2020, (Cited by: 7; All Open Access, Gold Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Vujacic-Mirski2020b,
title = {Development of an analytical assay for electrochemical detection and quantification of protein-bound 3-nitrotyrosine in biological samples and comparison with classical, antibody-based methods},
author = {Ksenija Vujacic-Mirski and Kai Bruns and Sanela Kalinovic and Matthias Oelze and Swenja Kröller-Schön and Sebastian Steven and Milos Mojovic and Bato Korac and Thomas Münzel and Andreas Daiber},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084254228&doi=10.3390%2fantiox9050388&partnerID=40&md5=9144982ce1a9eea136a9663ecf80df32},
doi = {10.3390/antiox9050388},
year = {2020},
date = {2020-01-01},
journal = {Antioxidants},
volume = {9},
number = {5},
abstract = {Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantified by electrochemical detection (ECD, CoulArray) and compared to classical methods, namely enzyme-linked immunosorbent assay (ELISA) and dot blot analysis using specific 3-NT antibodies. Calibration curves for authentic 3-NT (detection limit 10 nM) and a concentration-response pattern for 3-NT obtained from digested nitrated bovine serum albumin (BSA) were highly linear over a wide 3-NT concentration range. Also, ex vivo nitration of protein from heart, isolated mitochondria, and serum/plasma could be quantified using the HPLC/ECD method and was confirmed by LC-MS/MS. Of note, nitro-oxidative damage of mitochondria results in increased superoxide (O2 •–) formation rates (measured by dihydroethidium-based HPLC assay), pointing to a self-amplification mechanism of oxidative stress. Based on our ex vivo data, the CoulArray quantification method for 3-NT seems to have some advantages regarding sensitivity and selectivity. Establishing a reliable automated HPLC assay for the routine quantification of 3-NT in biological samples of cell culture, of animal and human origin seems to be more sophisticated than expected. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {Cited by: 7; All Open Access, Gold Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Reactive oxygen and nitrogen species (RONS) cause oxidative damage, which is associated with endothelial dysfunction and cardiovascular disease, but may also contribute to redox signaling. Therefore, their precise detection is important for the evaluation of disease mechanisms. Here, we compared three different methods for the detection of 3-nitrotyrosine (3-NT), a marker of nitro-oxidative stress, in biological samples. Nitrated proteins were generated by incubation with peroxynitrite or 3-morpholino sydnonimine (Sin-1) and subjected to total hydrolysis using pronase, a mixture of different proteases. The 3-NT was then separated by high performance liquid chromatography (HPLC) and quantified by electrochemical detection (ECD, CoulArray) and compared to classical methods, namely enzyme-linked immunosorbent assay (ELISA) and dot blot analysis using specific 3-NT antibodies. Calibration curves for authentic 3-NT (detection limit 10 nM) and a concentration-response pattern for 3-NT obtained from digested nitrated bovine serum albumin (BSA) were highly linear over a wide 3-NT concentration range. Also, ex vivo nitration of protein from heart, isolated mitochondria, and serum/plasma could be quantified using the HPLC/ECD method and was confirmed by LC-MS/MS. Of note, nitro-oxidative damage of mitochondria results in increased superoxide (O2 •–) formation rates (measured by dihydroethidium-based HPLC assay), pointing to a self-amplification mechanism of oxidative stress. Based on our ex vivo data, the CoulArray quantification method for 3-NT seems to have some advantages regarding sensitivity and selectivity. Establishing a reliable automated HPLC assay for the routine quantification of 3-NT in biological samples of cell culture, of animal and human origin seems to be more sophisticated than expected. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.