Please select year or author:
2005
Bačić, G.; Mojović, M.
EPR spin trapping of oxygen radicals in plants: A methodological overview Journal Article
In: Annals of the New York Academy of Sciences, vol. 1048, pp. 230-243, 2005, (cited By 26).
Abstract | Links | BibTeX | Tags:
@article{Bačić2005230,
title = {EPR spin trapping of oxygen radicals in plants: A methodological overview},
author = {G. Bačić and M. Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144448116&doi=10.1196%2fannals.1342.021&partnerID=40&md5=9546b82c7ac3c70df190c2a11beaa8cb},
doi = {10.1196/annals.1342.021},
year = {2005},
date = {2005-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1048},
pages = {230-243},
publisher = {New York Academy of Sciences},
abstract = {We present a brief account of the difficulties involved in detection of oxygen free radicals in plants and give a rationale for using the EPR spin trapping technique in such studies. Comparative analysis of characteristics of different spin traps is given, having in mind their suitability in trapping oxygen-centered free radicals. Certain technical aspects of EPR experiments related to successful trapping of free radicals are discussed. Previous studies of trapping of oxygen radicals in plants are reviewed in terms of how efficient the experimental approach employed has been in their detection and how this influences conclusions about the mechanisms of their production. In addition, we analyze the potential of spin labels in the analysis of free radical production in plants and demonstrate that the combination of EPR spin traps and spin labels is extremely efficient for this purpose. © 2005 New York Academy of Sciences.},
note = {cited By 26},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We present a brief account of the difficulties involved in detection of oxygen free radicals in plants and give a rationale for using the EPR spin trapping technique in such studies. Comparative analysis of characteristics of different spin traps is given, having in mind their suitability in trapping oxygen-centered free radicals. Certain technical aspects of EPR experiments related to successful trapping of free radicals are discussed. Previous studies of trapping of oxygen radicals in plants are reviewed in terms of how efficient the experimental approach employed has been in their detection and how this influences conclusions about the mechanisms of their production. In addition, we analyze the potential of spin labels in the analysis of free radical production in plants and demonstrate that the combination of EPR spin traps and spin labels is extremely efficient for this purpose. © 2005 New York Academy of Sciences.
Mojović, M.; Spasojević, I.; Vuletić, M.; Vučinić, Ž.; Bačić, G.
An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes Journal Article
In: Journal of the Serbian Chemical Society, vol. 70, no. 2, pp. 177-186, 2005, (cited By 10).
Abstract | Links | BibTeX | Tags:
@article{Mojović2005177,
title = {An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes},
author = {M. Mojović and I. Spasojević and M. Vuletić and Ž. Vučinić and G. Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144441582&doi=10.2298%2fJSC0502177M&partnerID=40&md5=2f12dac6d99a81a7aa36c81912db03b3},
doi = {10.2298/JSC0502177M},
year = {2005},
date = {2005-01-01},
journal = {Journal of the Serbian Chemical Society},
volume = {70},
number = {2},
pages = {177-186},
publisher = {Serbian Chemical Society},
abstract = {Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production can be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Tempone, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spin-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.},
note = {cited By 10},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production can be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Tempone, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spin-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.
Maksimović, V.; Mojović, M.; Neumann, G.; Vučinić, Ž.
Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction Journal Article
In: Annals of the New York Academy of Sciences, vol. 1048, pp. 461-465, 2005, (cited By 5).
Abstract | Links | BibTeX | Tags:
@article{Maksimović2005461,
title = {Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction},
author = {V. Maksimović and M. Mojović and G. Neumann and Ž. Vučinić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144438518&doi=10.1196%2fannals.1342.067&partnerID=40&md5=68a6ddb9953ecf969461a1912870520e},
doi = {10.1196/annals.1342.067},
year = {2005},
date = {2005-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1048},
pages = {461-465},
publisher = {New York Academy of Sciences},
abstract = {An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield. © 2005 New York Academy of Sciences.},
note = {cited By 5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield. © 2005 New York Academy of Sciences.
Mojović, M.; Vuletić, M.; Bačić, G. G.
Detection of oxygen-centered radicals using EPR spin-trap DEPMPO: The effect of oxygen Journal Article
In: Annals of the New York Academy of Sciences, vol. 1048, pp. 471-475, 2005, (cited By 18).
Abstract | Links | BibTeX | Tags:
@article{Mojović2005471,
title = {Detection of oxygen-centered radicals using EPR spin-trap DEPMPO: The effect of oxygen},
author = {M. Mojović and M. Vuletić and G. G. Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144438044&doi=10.1196%2fannals.1342.069&partnerID=40&md5=4fd9ec4cc020b2163dc91a51ef38f486},
doi = {10.1196/annals.1342.069},
year = {2005},
date = {2005-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1048},
pages = {471-475},
publisher = {New York Academy of Sciences},
abstract = {Studies of the ability of the EPR spin trap DEPMPO to detect both superoxide and hydroxyl radicals produced by systems in vitro and in vivo are presented. Experiments using free radical-generating systems confirmed the suitability of the EPR spin trapping technique but also revealed the existence of an undesirable conversion of DEPMPO/OOH into DEPMPO/OH adducts. The rate of conversion decreases with oxygenation, and the production of oxygen-centered radicals increases. However, this property of DEPMPO does not have a significant influence on its ability to independently detect radicals produced by plant plasma membranes. Since the adduct conversion appears to be rather slow compared to radical generation, we conclude that the DEPMPO spin-trap can be efficiently used for detection of oxygen-centered radicals produced by systems in vivo, as demonstrated for isolated plasma membranes. © 2005 New York Academy of Sciences.},
note = {cited By 18},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Studies of the ability of the EPR spin trap DEPMPO to detect both superoxide and hydroxyl radicals produced by systems in vitro and in vivo are presented. Experiments using free radical-generating systems confirmed the suitability of the EPR spin trapping technique but also revealed the existence of an undesirable conversion of DEPMPO/OOH into DEPMPO/OH adducts. The rate of conversion decreases with oxygenation, and the production of oxygen-centered radicals increases. However, this property of DEPMPO does not have a significant influence on its ability to independently detect radicals produced by plant plasma membranes. Since the adduct conversion appears to be rather slow compared to radical generation, we conclude that the DEPMPO spin-trap can be efficiently used for detection of oxygen-centered radicals produced by systems in vivo, as demonstrated for isolated plasma membranes. © 2005 New York Academy of Sciences.
Bačić, Goran; Mojović, Miloš
EPR spin trapping of oxygen radicals in plants: A methodological overview Journal Article
In: Annals of the New York Academy of Sciences, vol. 1048, pp. 230 – 243, 2005, (Cited by: 29).
Abstract | Links | BibTeX | Tags:
@article{Bačić2005230b,
title = {EPR spin trapping of oxygen radicals in plants: A methodological overview},
author = {Goran Bačić and Miloš Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144448116&doi=10.1196%2fannals.1342.021&partnerID=40&md5=9546b82c7ac3c70df190c2a11beaa8cb},
doi = {10.1196/annals.1342.021},
year = {2005},
date = {2005-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1048},
pages = {230 – 243},
abstract = {We present a brief account of the difficulties involved in detection of oxygen free radicals in plants and give a rationale for using the EPR spin trapping technique in such studies. Comparative analysis of characteristics of different spin traps is given, having in mind their suitability in trapping oxygen-centered free radicals. Certain technical aspects of EPR experiments related to successful trapping of free radicals are discussed. Previous studies of trapping of oxygen radicals in plants are reviewed in terms of how efficient the experimental approach employed has been in their detection and how this influences conclusions about the mechanisms of their production. In addition, we analyze the potential of spin labels in the analysis of free radical production in plants and demonstrate that the combination of EPR spin traps and spin labels is extremely efficient for this purpose. © 2005 New York Academy of Sciences.},
note = {Cited by: 29},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We present a brief account of the difficulties involved in detection of oxygen free radicals in plants and give a rationale for using the EPR spin trapping technique in such studies. Comparative analysis of characteristics of different spin traps is given, having in mind their suitability in trapping oxygen-centered free radicals. Certain technical aspects of EPR experiments related to successful trapping of free radicals are discussed. Previous studies of trapping of oxygen radicals in plants are reviewed in terms of how efficient the experimental approach employed has been in their detection and how this influences conclusions about the mechanisms of their production. In addition, we analyze the potential of spin labels in the analysis of free radical production in plants and demonstrate that the combination of EPR spin traps and spin labels is extremely efficient for this purpose. © 2005 New York Academy of Sciences.
Maksimović, Vuk; Mojović, Miloš; Neumann, Günter; Vučinić, Željko
Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction Journal Article
In: Annals of the New York Academy of Sciences, vol. 1048, pp. 461 – 465, 2005, (Cited by: 5).
Abstract | Links | BibTeX | Tags:
@article{Maksimović2005461b,
title = {Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction},
author = {Vuk Maksimović and Miloš Mojović and Günter Neumann and Željko Vučinić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144438518&doi=10.1196%2fannals.1342.067&partnerID=40&md5=68a6ddb9953ecf969461a1912870520e},
doi = {10.1196/annals.1342.067},
year = {2005},
date = {2005-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1048},
pages = {461 – 465},
abstract = {An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield. © 2005 New York Academy of Sciences.},
note = {Cited by: 5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield. © 2005 New York Academy of Sciences.
Mojović, Miloš; Spasojević, Ivan; Bačić, Goran
Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems Journal Article
In: Journal of Chemical Information and Modeling, vol. 45, no. 6, pp. 1716 – 1718, 2005, (Cited by: 6).
Abstract | Links | BibTeX | Tags:
@article{Mojović20051716b,
title = {Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems},
author = {Miloš Mojović and Ivan Spasojević and Goran Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28944439903&doi=10.1021%2fci050173d&partnerID=40&md5=ad8614aa10785184910cfbae82e481df},
doi = {10.1021/ci050173d},
year = {2005},
date = {2005-01-01},
journal = {Journal of Chemical Information and Modeling},
volume = {45},
number = {6},
pages = {1716 – 1718},
abstract = {We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (*H) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bačič, G.; Mojović, M. Ann. N. Y. Acad. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (*H) adduct i.e., DEPMPO/H. An electrochemical system known to produce only *H radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems. © 2005 American Chemical Society.},
note = {Cited by: 6},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (*H) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bačič, G.; Mojović, M. Ann. N. Y. Acad. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (*H) adduct i.e., DEPMPO/H. An electrochemical system known to produce only *H radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems. © 2005 American Chemical Society.
Veljović-Jovanović, Sonja; Kukavica, Biljana; Cvetić, Tijana; Mojović, Miloš; Vučinić, Željko
Ascorbic acid and the oxidative processes in pea root cell wall isolates: Characterization by fluorescence and EPR spectroscopy Journal Article
In: Annals of the New York Academy of Sciences, vol. 1048, pp. 500 – 504, 2005, (Cited by: 10).
Abstract | Links | BibTeX | Tags:
@article{Veljović-Jovanović2005500b,
title = {Ascorbic acid and the oxidative processes in pea root cell wall isolates: Characterization by fluorescence and EPR spectroscopy},
author = {Sonja Veljović-Jovanović and Biljana Kukavica and Tijana Cvetić and Miloš Mojović and Željko Vučinić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144470117&doi=10.1196%2fannals.1342.076&partnerID=40&md5=0d832399737d944722017d3ab82e5494},
doi = {10.1196/annals.1342.076},
year = {2005},
date = {2005-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1048},
pages = {500 – 504},
abstract = {A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals. © 2005 New York Academy of Sciences.},
note = {Cited by: 10},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals. © 2005 New York Academy of Sciences.
Mojović, Miloš; Spasojević, Ivan; Vuletić, Mirjana; Vučinić, Željko; Bačić, Goran
An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes Journal Article
In: Journal of the Serbian Chemical Society, vol. 70, no. 2, pp. 177 – 186, 2005, (Cited by: 11; All Open Access, Bronze Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Mojović2005177b,
title = {An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes},
author = {Miloš Mojović and Ivan Spasojević and Mirjana Vuletić and Željko Vučinić and Goran Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144441582&doi=10.2298%2fJSC0502177M&partnerID=40&md5=2f12dac6d99a81a7aa36c81912db03b3},
doi = {10.2298/JSC0502177M},
year = {2005},
date = {2005-01-01},
journal = {Journal of the Serbian Chemical Society},
volume = {70},
number = {2},
pages = {177 – 186},
abstract = {Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production can be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Tempone, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spin-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.},
note = {Cited by: 11; All Open Access, Bronze Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production can be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Tempone, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spin-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.
Mojović, Miloš; Vuletić, Mirjana; Bačić, Goran G.
Detection of oxygen-centered radicals using EPR spin-trap DEPMPO: The effect of oxygen Journal Article
In: Annals of the New York Academy of Sciences, vol. 1048, pp. 471 – 475, 2005, (Cited by: 19).
Abstract | Links | BibTeX | Tags:
@article{Mojović2005471b,
title = {Detection of oxygen-centered radicals using EPR spin-trap DEPMPO: The effect of oxygen},
author = {Miloš Mojović and Mirjana Vuletić and Goran G. Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25144438044&doi=10.1196%2fannals.1342.069&partnerID=40&md5=4fd9ec4cc020b2163dc91a51ef38f486},
doi = {10.1196/annals.1342.069},
year = {2005},
date = {2005-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1048},
pages = {471 – 475},
abstract = {Studies of the ability of the EPR spin trap DEPMPO to detect both superoxide and hydroxyl radicals produced by systems in vitro and in vivo are presented. Experiments using free radical-generating systems confirmed the suitability of the EPR spin trapping technique but also revealed the existence of an undesirable conversion of DEPMPO/OOH into DEPMPO/OH adducts. The rate of conversion decreases with oxygenation, and the production of oxygen-centered radicals increases. However, this property of DEPMPO does not have a significant influence on its ability to independently detect radicals produced by plant plasma membranes. Since the adduct conversion appears to be rather slow compared to radical generation, we conclude that the DEPMPO spin-trap can be efficiently used for detection of oxygen-centered radicals produced by systems in vivo, as demonstrated for isolated plasma membranes. © 2005 New York Academy of Sciences.},
note = {Cited by: 19},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Studies of the ability of the EPR spin trap DEPMPO to detect both superoxide and hydroxyl radicals produced by systems in vitro and in vivo are presented. Experiments using free radical-generating systems confirmed the suitability of the EPR spin trapping technique but also revealed the existence of an undesirable conversion of DEPMPO/OOH into DEPMPO/OH adducts. The rate of conversion decreases with oxygenation, and the production of oxygen-centered radicals increases. However, this property of DEPMPO does not have a significant influence on its ability to independently detect radicals produced by plant plasma membranes. Since the adduct conversion appears to be rather slow compared to radical generation, we conclude that the DEPMPO spin-trap can be efficiently used for detection of oxygen-centered radicals produced by systems in vivo, as demonstrated for isolated plasma membranes. © 2005 New York Academy of Sciences.
2004
Mojović, Miloš; Vuletić, Mirjana; Bačić, Goran G.; Vučinić, Željko
Oxygen radicals produced by plant plasma membranes: An EPR spin-trap study Journal Article
In: Journal of Experimental Botany, vol. 55, no. 408, pp. 2523 – 2531, 2004, (Cited by: 31; All Open Access, Bronze Open Access).
Abstract | Links | BibTeX | Tags:
@article{Mojović20042523b,
title = {Oxygen radicals produced by plant plasma membranes: An EPR spin-trap study},
author = {Miloš Mojović and Mirjana Vuletić and Goran G. Bačić and Željko Vučinić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-11144262180&doi=10.1093%2fjxb%2ferh266&partnerID=40&md5=89274629049d693354f57cd006e466d1},
doi = {10.1093/jxb/erh266},
year = {2004},
date = {2004-01-01},
journal = {Journal of Experimental Botany},
volume = {55},
number = {408},
pages = {2523 – 2531},
abstract = {Plant plasma membranes are known to produce superoxide radicals, while the production of the hydroxyl radical, previously detected in complex plant tissues, is thought to occur in the cell wall. The mechanism of production of superoxide radicals by plant plasma membranes is, however, under dispute. It is shown, using electron paramagnetic resonance spectroscopy with a 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide spintrap capable of differentiating between radical species, that isolated purified plasma membranes from maize roots produce hydroxyl radicals besides superoxide radicals. The results argue in favour of superoxide production through an oxygen and diphenylene iodonium-sensitive, NADH-dependent superoxide synthase mechanism, as well as through other unidentified mechanism(s). The hydroxyl radical is produced by an oxygen-insensitive, NADH-stimulated mechanism, which is enhanced in membranes in which the superoxide synthase is incapacitated by substrate removal or inhibition.},
note = {Cited by: 31; All Open Access, Bronze Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Plant plasma membranes are known to produce superoxide radicals, while the production of the hydroxyl radical, previously detected in complex plant tissues, is thought to occur in the cell wall. The mechanism of production of superoxide radicals by plant plasma membranes is, however, under dispute. It is shown, using electron paramagnetic resonance spectroscopy with a 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide spintrap capable of differentiating between radical species, that isolated purified plasma membranes from maize roots produce hydroxyl radicals besides superoxide radicals. The results argue in favour of superoxide production through an oxygen and diphenylene iodonium-sensitive, NADH-dependent superoxide synthase mechanism, as well as through other unidentified mechanism(s). The hydroxyl radical is produced by an oxygen-insensitive, NADH-stimulated mechanism, which is enhanced in membranes in which the superoxide synthase is incapacitated by substrate removal or inhibition.
Mojović, M.; Vuletić, M.; Bačić, G. G.; Vučinić, Ž.
Oxygen radicals produced by plant plasma membranes: An EPR spin-trap study Journal Article
In: Journal of Experimental Botany, vol. 55, no. 408, pp. 2523-2531, 2004, (cited By 30).
Abstract | Links | BibTeX | Tags:
@article{Mojović20042523,
title = {Oxygen radicals produced by plant plasma membranes: An EPR spin-trap study},
author = {M. Mojović and M. Vuletić and G. G. Bačić and Ž. Vučinić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-11144262180&doi=10.1093%2fjxb%2ferh266&partnerID=40&md5=89274629049d693354f57cd006e466d1},
doi = {10.1093/jxb/erh266},
year = {2004},
date = {2004-01-01},
journal = {Journal of Experimental Botany},
volume = {55},
number = {408},
pages = {2523-2531},
publisher = {Oxford University Press},
abstract = {Plant plasma membranes are known to produce superoxide radicals, while the production of the hydroxyl radical, previously detected in complex plant tissues, is thought to occur in the cell wall. The mechanism of production of superoxide radicals by plant plasma membranes is, however, under dispute. It is shown, using electron paramagnetic resonance spectroscopy with a 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide spintrap capable of differentiating between radical species, that isolated purified plasma membranes from maize roots produce hydroxyl radicals besides superoxide radicals. The results argue in favour of superoxide production through an oxygen and diphenylene iodonium-sensitive, NADH-dependent superoxide synthase mechanism, as well as through other unidentified mechanism(s). The hydroxyl radical is produced by an oxygen-insensitive, NADH-stimulated mechanism, which is enhanced in membranes in which the superoxide synthase is incapacitated by substrate removal or inhibition.},
note = {cited By 30},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Plant plasma membranes are known to produce superoxide radicals, while the production of the hydroxyl radical, previously detected in complex plant tissues, is thought to occur in the cell wall. The mechanism of production of superoxide radicals by plant plasma membranes is, however, under dispute. It is shown, using electron paramagnetic resonance spectroscopy with a 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide spintrap capable of differentiating between radical species, that isolated purified plasma membranes from maize roots produce hydroxyl radicals besides superoxide radicals. The results argue in favour of superoxide production through an oxygen and diphenylene iodonium-sensitive, NADH-dependent superoxide synthase mechanism, as well as through other unidentified mechanism(s). The hydroxyl radical is produced by an oxygen-insensitive, NADH-stimulated mechanism, which is enhanced in membranes in which the superoxide synthase is incapacitated by substrate removal or inhibition.
2000
Petković, J.; Mladenović, I.; Vukelić, N.; Mojović, M.; Bačić, G.
Lanthanide doped alkaline metal sulphates as candidates for EPR dosimetry Journal Article
In: Journal of the Serbian Chemical Society, vol. 65, no. 10, pp. 743-754, 2000, (cited By 0).
Abstract | Links | BibTeX | Tags:
@article{Petković2000743,
title = {Lanthanide doped alkaline metal sulphates as candidates for EPR dosimetry},
author = {J. Petković and I. Mladenović and N. Vukelić and M. Mojović and G. Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034345791&doi=10.2298%2fjsc0010743p&partnerID=40&md5=80728ed866a70109252945e70f2a451d},
doi = {10.2298/jsc0010743p},
year = {2000},
date = {2000-01-01},
journal = {Journal of the Serbian Chemical Society},
volume = {65},
number = {10},
pages = {743-754},
publisher = {Serbian Chemical Society},
abstract = {The applicability of lanthanide doped alkaline metal sulphates as a new type of dosimeters for EPR dosimetery of ionizing radiation has been investigated in an attempt to obtain a dosimeter with better characteristics than the commonly used alanine dosimeter. Irradiation of samples doped with different lanthanides (Y, Ln, Gd) showed that the best sensitivity is obtained using dosimeters doped with Y2(SO4)3. Different procedures for manufacturing dosimeters were studied and an optimum procedure was established. The time stability of the EPR signal of the irradiated Y2(SO4)3 dosimeter was investigated using a 15N-PDT standard and no fading of the EPR signal was observed over at least two weeks. The dose dependence of alanine and Y2(SO4)3 doped K3Na(SO4)2 dosimeters irradiated in the range 20 Gy - 200 kGy was analysed using a combination of 1-hit and 2-hit mechanisms of free radicals creation.},
note = {cited By 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The applicability of lanthanide doped alkaline metal sulphates as a new type of dosimeters for EPR dosimetery of ionizing radiation has been investigated in an attempt to obtain a dosimeter with better characteristics than the commonly used alanine dosimeter. Irradiation of samples doped with different lanthanides (Y, Ln, Gd) showed that the best sensitivity is obtained using dosimeters doped with Y2(SO4)3. Different procedures for manufacturing dosimeters were studied and an optimum procedure was established. The time stability of the EPR signal of the irradiated Y2(SO4)3 dosimeter was investigated using a 15N-PDT standard and no fading of the EPR signal was observed over at least two weeks. The dose dependence of alanine and Y2(SO4)3 doped K3Na(SO4)2 dosimeters irradiated in the range 20 Gy - 200 kGy was analysed using a combination of 1-hit and 2-hit mechanisms of free radicals creation.
Petković, Jelena; Mladenović, Ivana; Vukelić, Nikola; Mojović, Miloš; Bačić, Goran
Lanthanide doped alkaline metal sulphates as candidates for EPR dosimetry Journal Article
In: Journal of the Serbian Chemical Society, vol. 65, no. 10, pp. 743 – 754, 2000, (Cited by: 0; All Open Access, Bronze Open Access).
Abstract | Links | BibTeX | Tags:
@article{Petković2000743b,
title = {Lanthanide doped alkaline metal sulphates as candidates for EPR dosimetry},
author = {Jelena Petković and Ivana Mladenović and Nikola Vukelić and Miloš Mojović and Goran Bačić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034345791&doi=10.2298%2fjsc0010743p&partnerID=40&md5=80728ed866a70109252945e70f2a451d},
doi = {10.2298/jsc0010743p},
year = {2000},
date = {2000-01-01},
journal = {Journal of the Serbian Chemical Society},
volume = {65},
number = {10},
pages = {743 – 754},
abstract = {The applicability of lanthanide doped alkaline metal sulphates as a new type of dosimeters for EPR dosimetery of ionizing radiation has been investigated in an attempt to obtain a dosimeter with better characteristics than the commonly used alanine dosimeter. Irradiation of samples doped with different lanthanides (Y, Ln, Gd) showed that the best sensitivity is obtained using dosimeters doped with Y2(SO4)3. Different procedures for manufacturing dosimeters were studied and an optimum procedure was established. The time stability of the EPR signal of the irradiated Y2(SO4)3 dosimeter was investigated using a 15N-PDT standard and no fading of the EPR signal was observed over at least two weeks. The dose dependence of alanine and Y2(SO4)3 doped K3Na(SO4)2 dosimeters irradiated in the range 20 Gy - 200 kGy was analysed using a combination of 1-hit and 2-hit mechanisms of free radicals creation.},
note = {Cited by: 0; All Open Access, Bronze Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The applicability of lanthanide doped alkaline metal sulphates as a new type of dosimeters for EPR dosimetery of ionizing radiation has been investigated in an attempt to obtain a dosimeter with better characteristics than the commonly used alanine dosimeter. Irradiation of samples doped with different lanthanides (Y, Ln, Gd) showed that the best sensitivity is obtained using dosimeters doped with Y2(SO4)3. Different procedures for manufacturing dosimeters were studied and an optimum procedure was established. The time stability of the EPR signal of the irradiated Y2(SO4)3 dosimeter was investigated using a 15N-PDT standard and no fading of the EPR signal was observed over at least two weeks. The dose dependence of alanine and Y2(SO4)3 doped K3Na(SO4)2 dosimeters irradiated in the range 20 Gy - 200 kGy was analysed using a combination of 1-hit and 2-hit mechanisms of free radicals creation.