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2023
Milutinović, Milica; Nakarada, Đura; Božunović, Jelena; Todorović, Miloš; Gašić, Uroš; Živković, Suzana; Skorić, Marijana; Ivković, Đurđa; Savić, Jelena; Devrnja, Nina; Aničić, Neda; Banjanac, Tijana; Mojović, Miloš; Mišić, Danijela
Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening Journal Article
In: Antioxidants, vol. 12, no. 2, 2023, (Cited by: 1; All Open Access, Gold Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Milutinović2023b,
title = {Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening},
author = {Milica Milutinović and Đura Nakarada and Jelena Božunović and Miloš Todorović and Uroš Gašić and Suzana Živković and Marijana Skorić and Đurđa Ivković and Jelena Savić and Nina Devrnja and Neda Aničić and Tijana Banjanac and Miloš Mojović and Danijela Mišić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149231449&doi=10.3390%2fantiox12020346&partnerID=40&md5=16739c9e61a2f5d2bfb9a909d6ad207d},
doi = {10.3390/antiox12020346},
year = {2023},
date = {2023-01-01},
journal = {Antioxidants},
volume = {12},
number = {2},
abstract = {The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated. © 2023 by the authors.},
note = {Cited by: 1; All Open Access, Gold Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Božilović, Bojana; Nikolić, Bogdan; Waisi, Hadi; Trifković, Jelena; Dodevski, Vladimir; Janković, Bojan; Krstić, Sanja; Mojović, Miloš
In: Agronomy, vol. 13, no. 7, 2023, (Cited by: 0; All Open Access, Gold Open Access).
Abstract | Links | BibTeX | Tags:
@article{Božilović2023,
title = {Influence of 24-Epibrassinolide on the Energetic Parameters and Early Stages of Growth and Development in Seedlings of Two Maize (Zea mays L.) Genotypes},
author = {Bojana Božilović and Bogdan Nikolić and Hadi Waisi and Jelena Trifković and Vladimir Dodevski and Bojan Janković and Sanja Krstić and Miloš Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175115563&doi=10.3390%2fagronomy13071673&partnerID=40&md5=24684bb1feac960e5e5ad4c634b63533},
doi = {10.3390/agronomy13071673},
year = {2023},
date = {2023-01-01},
journal = {Agronomy},
volume = {13},
number = {7},
abstract = {Brassinosteroids (BRs) are a class of plant hormones that play important roles in regulating various physiological and developmental processes in plants. One of the most effective BRs involved in modulating crop growth is 24-epibrassinolide (24-EBL). The effects of different concentrations of 24-EBL on various biochemical and biophysical parameters critical to early growth stages and seedling development were investigated using two maize hybrids, ‘ZP 434’ (a new-generation hybrid) and ‘ZP 704’ (an older-generation hybrid). The evaluation of results is based on measurements of germination percentage, morphometric parameters, redox status, comparative analysis of thermodynamic parameters (such as Gibbs free energy, enthalpy, entropy), and the concentration of specific sugars in different parts of maize seedlings. The results indicate that the germination and initial growth of maize seedlings are influenced by the flow of crucial sugars from the remaining seed (as a source of nutrients) towards the plumule and radicle (as sink organs). Furthermore, alterations in Gibbs free energy play a significant role in these sugar transfers within the maize seedlings. The seed germination was most affected by the highest concentrations of 24-EBL, showing inhibitory effects, whereas lower and moderate concentrations of exogenously added 24-EBL exhibited a beneficial influence on the initial phases of seedling growth. The mentioned approach gives new insights into source–sink relationships and can be used as a quantitative measure of the germination energy, which until now has been a qualitative criterion in seed science. © 2023 by the authors.},
note = {Cited by: 0; All Open Access, Gold Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Milutinović, M.; Nakarada, Đ.; Božunović, J.; Todorović, M.; Gašić, U.; Živković, S.; Skorić, M.; Ivković, Đ.; Savić, J.; Devrnja, N.; Aničić, N.; Banjanac, T.; Mojović, M.; Mišić, D.
Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening Journal Article
In: Antioxidants, vol. 12, no. 2, 2023, (cited By 0).
Abstract | Links | BibTeX | Tags:
@article{Milutinović2023,
title = {Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening},
author = {M. Milutinović and Đ. Nakarada and J. Božunović and M. Todorović and U. Gašić and S. Živković and M. Skorić and Đ. Ivković and J. Savić and N. Devrnja and N. Aničić and T. Banjanac and M. Mojović and D. Mišić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149231449&doi=10.3390%2fantiox12020346&partnerID=40&md5=16739c9e61a2f5d2bfb9a909d6ad207d},
doi = {10.3390/antiox12020346},
year = {2023},
date = {2023-01-01},
journal = {Antioxidants},
volume = {12},
number = {2},
publisher = {MDPI},
abstract = {The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated. © 2023 by the authors.},
note = {cited By 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2022
Bartolić, Dragana; Mojović, Miloš; Prokopijević, Miloš; Djikanović, Daniela; Kalauzi, Aleksandar; Mutavdžić, Dragosav; Baošić, Rada; Radotić, Ksenija
Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study Journal Article
In: Journal of the Science of Food and Agriculture, vol. 102, no. 6, pp. 2500 – 2505, 2022, (Cited by: 1).
Abstract | Links | BibTeX | Tags:
@article{Bartolić20222500b,
title = {Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study},
author = {Dragana Bartolić and Miloš Mojović and Miloš Prokopijević and Daniela Djikanović and Aleksandar Kalauzi and Dragosav Mutavdžić and Rada Baošić and Ksenija Radotić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118771838&doi=10.1002%2fjsfa.11591&partnerID=40&md5=0c702ebc1e1f64180a75b337db6ff19a},
doi = {10.1002/jsfa.11591},
year = {2022},
date = {2022-01-01},
journal = {Journal of the Science of Food and Agriculture},
volume = {102},
number = {6},
pages = {2500 – 2505},
abstract = {BACKGROUND: Aflatoxin B1 (AFB1) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB1 and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB1-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB1. RESULTS: Different changes in the content of lignin and free radicals with increasing AFB1 concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923},
note = {Cited by: 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Milosavljević, Dragica M.; Maksimović, Vuk M.; Milivojević, Jasminka M.; Nakarada, Đura J.; Mojović, Miloš D.; Maksimović, Jelena J. Dragišić
Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars Journal Article
In: Plants, vol. 11, no. 24, 2022, (Cited by: 1; All Open Access, Gold Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Milosavljević2022b,
title = {Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars},
author = {Dragica M. Milosavljević and Vuk M. Maksimović and Jasminka M. Milivojević and Đura J. Nakarada and Miloš D. Mojović and Jelena J. Dragišić Maksimović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144957209&doi=10.3390%2fplants11243566&partnerID=40&md5=1cbb6cf17d855eb4d9a4fe969e2fa6d6},
doi = {10.3390/plants11243566},
year = {2022},
date = {2022-01-01},
journal = {Plants},
volume = {11},
number = {24},
abstract = {Phenolic compounds of 25 newly introduced strawberry cultivars were profiled using spectrophotometry, electron paramagnetic resonance (EPR) spectroscopy, and high-performance liquid chromatography-mass spectrometry. Total phenolic and anthocyanin content (TPC and TACY, respectively), as well as vitamin C, and concentrations of individual phenolic compounds in fruits were evaluated to identify the most promising cultivars according to their phenolic profile. The highest values of TPC, TACY, and vitamin C were recorded in ‘Premy’ (1.53 mg eq GA g−1 FW), ‘Sandra’ (30.60 mg eq Pg-3-g 100 g−1 FW), and ‘Laetitia’ (56.32 mg 100 g−1 FW), respectively. The DPPH and •OH radicals scavenging activity of fruit methanolic extracts was estimated using EPR spectroscopy. All cultivars are almost uniformly effective in the scavenging of •OH radical, while ‘Tea’, ‘Premy’, and ‘Joly’ were marked as highly potent cultivars (over 70%) in terms of DPPH-antiradical activity. Specific peroxidase activities were the highest in ‘Garda’, ‘Federica’, and ‘Rumba’ (0.11, 0.08, and 0.06 U mg−1 prot, respectively). ‘Laetitia’, ‘Joly’, ‘Arianna’, ‘Tea’, and ‘Mila’ cultivars were distinguished from others as the richest concerning almost all flavonoids and phenolic acids, including some other parameters of bioactivity. These cultivars could be recommended to consumers as functional fruit foods. © 2022 by the authors.},
note = {Cited by: 1; All Open Access, Gold Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pagnacco, Maja; Marković, Smilja; Potočnik, Jelena; Krstić, Vesna; Tančić, Pavle; Mojović, Miloš; Mojović, Zorica
The Influence of Electrode Constituents on Hydrogen Evolution Reaction on Phosphate W- and Mo-Bronze-Based Electrodes Journal Article
In: Journal of the Electrochemical Society, vol. 169, no. 10, 2022, (Cited by: 1; All Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Pagnacco2022b,
title = {The Influence of Electrode Constituents on Hydrogen Evolution Reaction on Phosphate W- and Mo-Bronze-Based Electrodes},
author = {Maja Pagnacco and Smilja Marković and Jelena Potočnik and Vesna Krstić and Pavle Tančić and Miloš Mojović and Zorica Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140306202&doi=10.1149%2f1945-7111%2fac96ab&partnerID=40&md5=55812e236eeeb9b3f86b307056e07250},
doi = {10.1149/1945-7111/ac96ab},
year = {2022},
date = {2022-01-01},
journal = {Journal of the Electrochemical Society},
volume = {169},
number = {10},
abstract = {Phosphate tungsten bronze (WPB) and phosphate molybdenum bronze (MoPB) were synthesized and modified with rhenium. The existing phases were established by X-ray powder diffraction (XRPD), electron paramagnetic spectroscopy (EPR) and Field emission scanning electron microscopy (FESEM). The electroactivity of bronze samples, with and without rhenium for hydrogen evolution reaction (HER) was tested. The influence of carbon black presence in the catalytic ink on the electrochemical activity was investigated. Collected results provide insight into the effects of the constituents of an electrode on its electrochemical activity. © 2022 The Electrochemical Society (“ECS”). Published on behalf of ECS by IOP Publishing Limited.},
note = {Cited by: 1; All Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bartolić, D.; Mojović, M.; Prokopijević, M.; Djikanović, D.; Kalauzi, A.; Mutavdžić, D.; Baošić, R.; Radotić, K.
Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study Journal Article
In: Journal of the Science of Food and Agriculture, vol. 102, no. 6, pp. 2500-2505, 2022, (cited By 1).
Abstract | Links | BibTeX | Tags:
@article{Bartolić20222500,
title = {Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study},
author = {D. Bartolić and M. Mojović and M. Prokopijević and D. Djikanović and A. Kalauzi and D. Mutavdžić and R. Baošić and K. Radotić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118771838&doi=10.1002%2fjsfa.11591&partnerID=40&md5=0c702ebc1e1f64180a75b337db6ff19a},
doi = {10.1002/jsfa.11591},
year = {2022},
date = {2022-01-01},
journal = {Journal of the Science of Food and Agriculture},
volume = {102},
number = {6},
pages = {2500-2505},
publisher = {John Wiley and Sons Ltd},
abstract = {BACKGROUND: Aflatoxin B1 (AFB1) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB1 and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB1-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB1. RESULTS: Different changes in the content of lignin and free radicals with increasing AFB1 concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923},
note = {cited By 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pagnacco, M.; Marković, S.; Potočnik, J.; Krstić, V.; Tančić, P.; Mojović, M.; Mojović, Z.
The Influence of Electrode Constituents on Hydrogen Evolution Reaction on Phosphate W- and Mo-Bronze-Based Electrodes Journal Article
In: Journal of the Electrochemical Society, vol. 169, no. 10, 2022, (cited By 0).
Abstract | Links | BibTeX | Tags:
@article{Pagnacco2022,
title = {The Influence of Electrode Constituents on Hydrogen Evolution Reaction on Phosphate W- and Mo-Bronze-Based Electrodes},
author = {M. Pagnacco and S. Marković and J. Potočnik and V. Krstić and P. Tančić and M. Mojović and Z. Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140306202&doi=10.1149%2f1945-7111%2fac96ab&partnerID=40&md5=55812e236eeeb9b3f86b307056e07250},
doi = {10.1149/1945-7111/ac96ab},
year = {2022},
date = {2022-01-01},
journal = {Journal of the Electrochemical Society},
volume = {169},
number = {10},
publisher = {Institute of Physics},
abstract = {Phosphate tungsten bronze (WPB) and phosphate molybdenum bronze (MoPB) were synthesized and modified with rhenium. The existing phases were established by X-ray powder diffraction (XRPD), electron paramagnetic spectroscopy (EPR) and Field emission scanning electron microscopy (FESEM). The electroactivity of bronze samples, with and without rhenium for hydrogen evolution reaction (HER) was tested. The influence of carbon black presence in the catalytic ink on the electrochemical activity was investigated. Collected results provide insight into the effects of the constituents of an electrode on its electrochemical activity. © 2022 The Electrochemical Society (“ECS”). Published on behalf of ECS by IOP Publishing Limited.},
note = {cited By 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Milosavljević, D. M.; Maksimović, V. M.; Milivojević, J. M.; Nakarada, Đ. J.; Mojović, M. D.; Maksimović, J. J. Dragišić
Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars Journal Article
In: Plants, vol. 11, no. 24, 2022, (cited By 0).
Abstract | Links | BibTeX | Tags:
@article{Milosavljević2022,
title = {Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars},
author = {D. M. Milosavljević and V. M. Maksimović and J. M. Milivojević and Đ. J. Nakarada and M. D. Mojović and J. J. Dragišić Maksimović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144957209&doi=10.3390%2fplants11243566&partnerID=40&md5=1cbb6cf17d855eb4d9a4fe969e2fa6d6},
doi = {10.3390/plants11243566},
year = {2022},
date = {2022-01-01},
journal = {Plants},
volume = {11},
number = {24},
publisher = {MDPI},
abstract = {Phenolic compounds of 25 newly introduced strawberry cultivars were profiled using spectrophotometry, electron paramagnetic resonance (EPR) spectroscopy, and high-performance liquid chromatography-mass spectrometry. Total phenolic and anthocyanin content (TPC and TACY, respectively), as well as vitamin C, and concentrations of individual phenolic compounds in fruits were evaluated to identify the most promising cultivars according to their phenolic profile. The highest values of TPC, TACY, and vitamin C were recorded in ‘Premy’ (1.53 mg eq GA g−1 FW), ‘Sandra’ (30.60 mg eq Pg-3-g 100 g−1 FW), and ‘Laetitia’ (56.32 mg 100 g−1 FW), respectively. The DPPH and •OH radicals scavenging activity of fruit methanolic extracts was estimated using EPR spectroscopy. All cultivars are almost uniformly effective in the scavenging of •OH radical, while ‘Tea’, ‘Premy’, and ‘Joly’ were marked as highly potent cultivars (over 70%) in terms of DPPH-antiradical activity. Specific peroxidase activities were the highest in ‘Garda’, ‘Federica’, and ‘Rumba’ (0.11, 0.08, and 0.06 U mg−1 prot, respectively). ‘Laetitia’, ‘Joly’, ‘Arianna’, ‘Tea’, and ‘Mila’ cultivars were distinguished from others as the richest concerning almost all flavonoids and phenolic acids, including some other parameters of bioactivity. These cultivars could be recommended to consumers as functional fruit foods. © 2022 by the authors.},
note = {cited By 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2021
Vesković, Ana; Nakarada, Đura; Pavićević, Aleksandra; Prokić, Bogomir; Perović, Milka; Kanazir, Selma; Popović-Bijelić, Ana; Mojović, Miloš
In vivo/ex vivo EPR investigation of the brain redox status and blood-brain barrier integrity in the 5xFAD mouse model of Alzheimer's disease Journal Article
In: Current Alzheimer Research, vol. 18, no. 1, pp. 25 – 34, 2021, (Cited by: 2).
Abstract | Links | BibTeX | Tags:
@article{Vesković202125b,
title = {In vivo/ex vivo EPR investigation of the brain redox status and blood-brain barrier integrity in the 5xFAD mouse model of Alzheimer's disease},
author = {Ana Vesković and Đura Nakarada and Aleksandra Pavićević and Bogomir Prokić and Milka Perović and Selma Kanazir and Ana Popović-Bijelić and Miloš Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104293047&doi=10.2174%2f1567205018666210324121156&partnerID=40&md5=49c60056dd19f58d7aebc09a3518eae1},
doi = {10.2174/1567205018666210324121156},
year = {2021},
date = {2021-01-01},
journal = {Current Alzheimer Research},
volume = {18},
number = {1},
pages = {25 – 34},
abstract = {Background: Alzheimer’s disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the research focus from Aβ to other pathological features of the disease. Objective: The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. Methods: In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibiting different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectroscopy was employed to investigate the brain metal content. Results: This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-mediated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. Conclusion: The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neuronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spectroscopy application in preclinical studies of neurodegenerative diseases. © 2021 Bentham Science Publishers.},
note = {Cited by: 2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Petrović, Srdjan; Stanković, Miroslav; Pavlović, Stefan; Mojović, Zorica; Radić, Nenad; Mojović, Miloš; Rožić, Ljiljana
Nickel oxide on mechanochemically synthesized TiO2–CeO2: photocatalytic and electrochemical activity Journal Article
In: Reaction Kinetics, Mechanisms and Catalysis, vol. 133, no. 2, pp. 1097 – 1110, 2021, (Cited by: 4).
Abstract | Links | BibTeX | Tags:
@article{Petrović20211097b,
title = {Nickel oxide on mechanochemically synthesized TiO2–CeO2: photocatalytic and electrochemical activity},
author = {Srdjan Petrović and Miroslav Stanković and Stefan Pavlović and Zorica Mojović and Nenad Radić and Miloš Mojović and Ljiljana Rožić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108609286&doi=10.1007%2fs11144-021-02014-8&partnerID=40&md5=77b077247e58e48489f41b8a9f6b6b14},
doi = {10.1007/s11144-021-02014-8},
year = {2021},
date = {2021-01-01},
journal = {Reaction Kinetics, Mechanisms and Catalysis},
volume = {133},
number = {2},
pages = {1097 – 1110},
abstract = {This paper presents a new two-step method for the synthesis of NiO/TiO2–CeO2 semiconductors, which implies, the first, the mechanochemical synthesis of TiO2–CeO2 support, and the second is the doping of different NiO contents by chemical precipitation in an aqueous medium in an ambient atmosphere. Structural, optical, electrochemical and photocatalytic properties of NiO/TiO2–CeO2 semiconductors were examined. The influence of different NiO contents on the electrocatalytic activity of NiO/TiO2–CeO2 samples was investigated in the oxygen evolution reaction and the results showed that the samples with the lowest NiO content have the highest electroactivity. The photocatalytic activity was determined during the phenol decomposition process over the samples with different NiO:TiO2–CeO2 ratios and the best photocatalytic activity is observed for the sample with 4.1 mol% NiO. The sample with the lowest NiO content obtained by a relatively simple method is suitable for use both as an electrode material in the oxygen evolution reaction and as a photocatalyst in the phenol decomposition process. © 2021, Akadémiai Kiadó, Budapest, Hungary.},
note = {Cited by: 4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Maksimović, Jelena Dragišić; Mojović, Miloš; Vučinić, Željko; Maksimović, Vuk
Spatial distribution of apoplastic antioxidative constituents in maize root Journal Article
In: Physiologia Plantarum, vol. 173, no. 3, pp. 818 – 828, 2021, (Cited by: 3).
Abstract | Links | BibTeX | Tags:
@article{DragišićMaksimović2021818b,
title = {Spatial distribution of apoplastic antioxidative constituents in maize root},
author = {Jelena Dragišić Maksimović and Miloš Mojović and Željko Vučinić and Vuk Maksimović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108365280&doi=10.1111%2fppl.13476&partnerID=40&md5=25d74508b1a75aa19bf65104d3635b34},
doi = {10.1111/ppl.13476},
year = {2021},
date = {2021-01-01},
journal = {Physiologia Plantarum},
volume = {173},
number = {3},
pages = {818 – 828},
abstract = {Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showed a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial–temporal changes in small regions of the intact root tissue. © 2021 Scandinavian Plant Physiology Society},
note = {Cited by: 3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Matijević, Milica; Žakula, Jelena; Korićanac, Lela; Radoičić, Marija; Liang, Xinyue; Mi, Lan; Tričković, Jelena Filipović; Šobot, Ana Valenta; Stanković, Maja N.; Nakarada, Đura; Mojović, Miloš; Petković, Marijana; Stepić, Milutin; Nešić, Maja D.
Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles Journal Article
In: Photochemical and Photobiological Sciences, vol. 20, no. 8, pp. 1087 – 1098, 2021, (Cited by: 3; All Open Access, Bronze Open Access).
Abstract | Links | BibTeX | Tags:
@article{Matijević20211087b,
title = {Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles},
author = {Milica Matijević and Jelena Žakula and Lela Korićanac and Marija Radoičić and Xinyue Liang and Lan Mi and Jelena Filipović Tričković and Ana Valenta Šobot and Maja N. Stanković and Đura Nakarada and Miloš Mojović and Marijana Petković and Milutin Stepić and Maja D. Nešić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85112557363&doi=10.1007%2fs43630-021-00082-2&partnerID=40&md5=13e1274c257164a0fd80c90cb86a6ad9},
doi = {10.1007/s43630-021-00082-2},
year = {2021},
date = {2021-01-01},
journal = {Photochemical and Photobiological Sciences},
volume = {20},
number = {8},
pages = {1087 – 1098},
abstract = {Abstract: In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy. Graphic abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.},
note = {Cited by: 3; All Open Access, Bronze Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Krunić, Matija; Ristić, Biljana; Bošnjak, Mihajlo; Paunović, Verica; Tovilović-Kovačević, Gordana; Zogović, Nevena; Mirčić, Aleksandar; Marković, Zoran; Todorović-Marković, Biljana; Jovanović, Svetlana; Kleut, Duška; Mojović, Miloš; Nakarada, Đura; Marković, Olivera; Vuković, Irena; Harhaji-Trajković, Ljubica; Trajković, Vladimir
Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death Journal Article
In: Free Radical Biology and Medicine, vol. 177, pp. 167 – 180, 2021, (Cited by: 5; All Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Krunić2021167b,
title = {Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death},
author = {Matija Krunić and Biljana Ristić and Mihajlo Bošnjak and Verica Paunović and Gordana Tovilović-Kovačević and Nevena Zogović and Aleksandar Mirčić and Zoran Marković and Biljana Todorović-Marković and Svetlana Jovanović and Duška Kleut and Miloš Mojović and Đura Nakarada and Olivera Marković and Irena Vuković and Ljubica Harhaji-Trajković and Vladimir Trajković},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117824181&doi=10.1016%2fj.freeradbiomed.2021.10.025&partnerID=40&md5=dca6b01a062016c9894c25afee0a0369},
doi = {10.1016/j.freeradbiomed.2021.10.025},
year = {2021},
date = {2021-01-01},
journal = {Free Radical Biology and Medicine},
volume = {177},
pages = {167 – 180},
abstract = {We investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP). GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•−), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagy-limiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy. © 2021},
note = {Cited by: 5; All Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Cvjetinović, Đorđe; Prijović, Željko; Janković, Drina; Radović, Magdalena; Mirković, Marija; Milanović, Zorana; Mojović, Miloš; Škalamera, Đani; Vranješ-Đurić, Sanja
Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking Journal Article
In: Journal of Controlled Release, vol. 332, pp. 301 – 311, 2021, (Cited by: 19).
Abstract | Links | BibTeX | Tags:
@article{Cvjetinović2021301b,
title = {Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking},
author = {Đorđe Cvjetinović and Željko Prijović and Drina Janković and Magdalena Radović and Marija Mirković and Zorana Milanović and Miloš Mojović and Đani Škalamera and Sanja Vranješ-Đurić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102414049&doi=10.1016%2fj.jconrel.2021.03.006&partnerID=40&md5=e3d7f2072a0b004f8d726c2358b83d71},
doi = {10.1016/j.jconrel.2021.03.006},
year = {2021},
date = {2021-01-01},
journal = {Journal of Controlled Release},
volume = {332},
pages = {301 – 311},
abstract = {Liposomes are promising drug's delivery systems due to decreased toxicity of the liposome-encapsulated drug, but wider clinical application requires their more efficient tumor targeting with uptake, controlled drug release and higher shelf life. The unique metabolic characteristics of cancer cells based on higher demand for energy and therefore increased glucose utilization were exploited in the design of glucose modified liposomes (GML) with the aim to provide increased tumor targeting via glucose transporters and increased ability of drug delivery into tumor cells. Tumor accumulating potential of GML and non-glucose liposomes (NGL) were investigated on CT26 and LS174T tumor-bearing mice by simple and reliable radiotracer method using 177Lu as radioactive marker. Both liposomes, GML and NGL were radiolabeled in high radiolabeling yield, showing high in vitro stability in biological media, as the main prerequisite for the biodistribution studies. Tumors displayed significantly better accumulation of 177Lu-GML with the maximum uptake 6 h post-injection (5.8 ± 0.2%/g in LS174T tumor and 5.1 ± 0.5%/g in CT26 tumor), compared to negligible uptake of 177Lu-NGL (0.6 ± 0.1%/g in LS174T tumor and 0.9 ± 0.2%/g in CT26 tumor). Results of comparative biodistribution studies of 177Lu-NGL and 177Lu-GML indicate that increased accumulation of GML is enabled by glucose transporters and subsequent endocytosis, resulting in their prolonged retention in tumor tissues (up to 72 h). Direct radiolabeling of liposomes with 177Lu may be used not only for biodistribution studies using radiotracking, but also for cancer treatment. © 2021 Elsevier B.V.},
note = {Cited by: 19},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Grippo, Valentina; Mojovic, Milos; Pavicevic, Aleksandra; Kabelac, Martin; Hubatka, Frantisek; Turanek, Jaroslav; Zatloukalova, Martina; Freeman, Bruce A.; Vacek, Jan
Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes Journal Article
In: Redox Biology, vol. 38, 2021, (Cited by: 15; All Open Access, Gold Open Access, Green Open Access).
Abstract | Links | BibTeX | Tags:
@article{Grippo2021b,
title = {Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes},
author = {Valentina Grippo and Milos Mojovic and Aleksandra Pavicevic and Martin Kabelac and Frantisek Hubatka and Jaroslav Turanek and Martina Zatloukalova and Bruce A. Freeman and Jan Vacek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096158093&doi=10.1016%2fj.redox.2020.101756&partnerID=40&md5=2da8f31c4fb805691910ec16078ae42b},
doi = {10.1016/j.redox.2020.101756},
year = {2021},
date = {2021-01-01},
journal = {Redox Biology},
volume = {38},
abstract = {Fatty acid nitroalkenes (NO2-FA) are endogenously-generated products of the reaction of metabolic and inflammatory-derived nitrogen dioxide (.NO2) with unsaturated fatty acids. These species mediate signaling actions and induce adaptive responses in preclinical models of inflammatory and metabolic diseases. The nitroalkene substituent possesses an electrophilic nature, resulting in rapid and reversible reactions with biological nucleophiles such as cysteine, thus supporting post-translational modifications (PTM) of proteins having susceptible nucleophilic centers. These reactions contribute to enzyme regulation, modulation of inflammation and cell proliferation and the regulation of gene expression responses. Herein, focus is placed on the reduction-oxidation (redox) characteristics and stability of specific NO2-FA regioisomers having biological and clinical relevance; nitro-oleic acid (NO2-OA), bis-allylic nitro-linoleic acid (NO2-LA) and the conjugated diene-containing nitro-conjugated linoleic acid (NO2-cLA). Cyclic and alternating-current voltammetry and chronopotentiometry were used to the study of reduction potentials of these NO2-FA. R–NO2 reduction was observed around −0.8 V (vs. Ag/AgCl/3 M KCl) and is related to relative NO2-FA electrophilicity. This reduction process could be utilized for the evaluation of NO2-FA stability in aqueous milieu, shown herein to be pH dependent. In addition, electron paramagnetic resonance (EPR) spectroscopy was used to define the stability of the nitroalkene moiety under aqueous conditions, specifically under conditions where nitric oxide (.NO) release could be detected. The experimental data were supported by density functional theory calculations using 6–311++G (d,p) basis set and B3LYP functional. Based on experimental and computational approaches, the relative electrophilicities of these NO2-FA are NO2-cLA >> NO2-LA > NO2-OA. Micellarization and vesiculation largely define these biophysical characteristics in aqueous, nucleophile-free conditions. At concentrations below the critical micellar concentration (CMC), monomeric NO2-FA predominate, while at greater concentrations a micellar phase consisting of self-assembled lipid structures predominates. The CMC, determined by dynamic light scattering in 0.1 M phosphate buffer (pH 7.4) at 25 °C, was 6.9 (NO2-LA) 10.6 (NO2-OA) and 42.3 μM (NO2-cLA), respectively. In aggregate, this study provides new insight into the biophysical properties of NO2-FA that are important for better understanding the cell signaling and pharmacological potential of this class of mediators. © 2020 The Authors},
note = {Cited by: 15; All Open Access, Gold Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Mojović, Zorica; Petrović, Srdjan; Mojović, Miloš; Pavlović, Stefan; Rožić, Ljiljana
Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection Journal Article
In: Journal of Physics and Chemistry of Solids, vol. 148, 2021, (Cited by: 5).
Abstract | Links | BibTeX | Tags:
@article{Mojović2021b,
title = {Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection},
author = {Zorica Mojović and Srdjan Petrović and Miloš Mojović and Stefan Pavlović and Ljiljana Rožić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089660184&doi=10.1016%2fj.jpcs.2020.109649&partnerID=40&md5=2178ca9e6a6a97bf29af47c7530f08d5},
doi = {10.1016/j.jpcs.2020.109649},
year = {2021},
date = {2021-01-01},
journal = {Journal of Physics and Chemistry of Solids},
volume = {148},
abstract = {In this paper, the relationship of structure, type of oxygen defects, valence states of Cr and Ru, and electrochemical properties of La0.7Sr0.3Cr1-XRuXO3 (0 < x < 0.1) perovskite-type oxides were studied. The samples were synthesized by a conventional solid-state reaction method and characterized by powder X-ray diffraction, X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and electron paramagnetic resonance spectroscopy. The electrochemical performance of these samples was evaluated towards the detection of 4-nitrophenol and strongly depends on the concentration of ruthenium incorporated in the crystal lattice. A new La0.7Sr0.3Cr0.925Ru0.075O3 electrocatalyst with a wide linear concentration range (25–5000 mM) and a detection limit of 8 mM was obtained. These results provide an opportunity for its application in the analysis of real water samples. © 2020 Elsevier Ltd},
note = {Cited by: 5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Mojović, Z.; Petrović, S.; Mojović, M.; Pavlović, S.; Rožić, L.
Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection Journal Article
In: Journal of Physics and Chemistry of Solids, vol. 148, 2021, (cited By 4).
Abstract | Links | BibTeX | Tags:
@article{Mojović2021,
title = {Ruthenium containing perovskites as electrode materials for 4-nitrophenol detection},
author = {Z. Mojović and S. Petrović and M. Mojović and S. Pavlović and L. Rožić},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089660184&doi=10.1016%2fj.jpcs.2020.109649&partnerID=40&md5=2178ca9e6a6a97bf29af47c7530f08d5},
doi = {10.1016/j.jpcs.2020.109649},
year = {2021},
date = {2021-01-01},
journal = {Journal of Physics and Chemistry of Solids},
volume = {148},
publisher = {Elsevier Ltd},
abstract = {In this paper, the relationship of structure, type of oxygen defects, valence states of Cr and Ru, and electrochemical properties of La0.7Sr0.3Cr1-XRuXO3 (0 < x < 0.1) perovskite-type oxides were studied. The samples were synthesized by a conventional solid-state reaction method and characterized by powder X-ray diffraction, X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and electron paramagnetic resonance spectroscopy. The electrochemical performance of these samples was evaluated towards the detection of 4-nitrophenol and strongly depends on the concentration of ruthenium incorporated in the crystal lattice. A new La0.7Sr0.3Cr0.925Ru0.075O3 electrocatalyst with a wide linear concentration range (25–5000 mM) and a detection limit of 8 mM was obtained. These results provide an opportunity for its application in the analysis of real water samples. © 2020 Elsevier Ltd},
note = {cited By 4},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Grippo, V.; Mojovic, M.; Pavicevic, A.; Kabelac, M.; Hubatka, F.; Turanek, J.; Zatloukalova, M.; Freeman, B. A.; Vacek, J.
Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes Journal Article
In: Redox Biology, vol. 38, 2021, (cited By 13).
Abstract | Links | BibTeX | Tags:
@article{Grippo2021,
title = {Electrophilic characteristics and aqueous behavior of fatty acid nitroalkenes},
author = {V. Grippo and M. Mojovic and A. Pavicevic and M. Kabelac and F. Hubatka and J. Turanek and M. Zatloukalova and B. A. Freeman and J. Vacek},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096158093&doi=10.1016%2fj.redox.2020.101756&partnerID=40&md5=2da8f31c4fb805691910ec16078ae42b},
doi = {10.1016/j.redox.2020.101756},
year = {2021},
date = {2021-01-01},
journal = {Redox Biology},
volume = {38},
publisher = {Elsevier B.V.},
abstract = {Fatty acid nitroalkenes (NO2-FA) are endogenously-generated products of the reaction of metabolic and inflammatory-derived nitrogen dioxide (.NO2) with unsaturated fatty acids. These species mediate signaling actions and induce adaptive responses in preclinical models of inflammatory and metabolic diseases. The nitroalkene substituent possesses an electrophilic nature, resulting in rapid and reversible reactions with biological nucleophiles such as cysteine, thus supporting post-translational modifications (PTM) of proteins having susceptible nucleophilic centers. These reactions contribute to enzyme regulation, modulation of inflammation and cell proliferation and the regulation of gene expression responses. Herein, focus is placed on the reduction-oxidation (redox) characteristics and stability of specific NO2-FA regioisomers having biological and clinical relevance; nitro-oleic acid (NO2-OA), bis-allylic nitro-linoleic acid (NO2-LA) and the conjugated diene-containing nitro-conjugated linoleic acid (NO2-cLA). Cyclic and alternating-current voltammetry and chronopotentiometry were used to the study of reduction potentials of these NO2-FA. R–NO2 reduction was observed around −0.8 V (vs. Ag/AgCl/3 M KCl) and is related to relative NO2-FA electrophilicity. This reduction process could be utilized for the evaluation of NO2-FA stability in aqueous milieu, shown herein to be pH dependent. In addition, electron paramagnetic resonance (EPR) spectroscopy was used to define the stability of the nitroalkene moiety under aqueous conditions, specifically under conditions where nitric oxide (.NO) release could be detected. The experimental data were supported by density functional theory calculations using 6–311++G (d,p) basis set and B3LYP functional. Based on experimental and computational approaches, the relative electrophilicities of these NO2-FA are NO2-cLA >> NO2-LA > NO2-OA. Micellarization and vesiculation largely define these biophysical characteristics in aqueous, nucleophile-free conditions. At concentrations below the critical micellar concentration (CMC), monomeric NO2-FA predominate, while at greater concentrations a micellar phase consisting of self-assembled lipid structures predominates. The CMC, determined by dynamic light scattering in 0.1 M phosphate buffer (pH 7.4) at 25 °C, was 6.9 (NO2-LA) 10.6 (NO2-OA) and 42.3 μM (NO2-cLA), respectively. In aggregate, this study provides new insight into the biophysical properties of NO2-FA that are important for better understanding the cell signaling and pharmacological potential of this class of mediators. © 2020 The Authors},
note = {cited By 13},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Vesković, A.; Nakarada, Đ.; Pavićević, A.; Prokić, B.; Perović, M.; Kanazir, S.; Popović-Bijelić, A.; Mojović, M.
In vivo/ex vivo EPR investigation of the brain redox status and blood-brain barrier integrity in the 5xFAD mouse model of Alzheimer's disease Journal Article
In: Current Alzheimer Research, vol. 18, no. 1, pp. 25-34, 2021, (cited By 1).
Abstract | Links | BibTeX | Tags:
@article{Vesković202125,
title = {In vivo/ex vivo EPR investigation of the brain redox status and blood-brain barrier integrity in the 5xFAD mouse model of Alzheimer's disease},
author = {A. Vesković and Đ. Nakarada and A. Pavićević and B. Prokić and M. Perović and S. Kanazir and A. Popović-Bijelić and M. Mojović},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104293047&doi=10.2174%2f1567205018666210324121156&partnerID=40&md5=49c60056dd19f58d7aebc09a3518eae1},
doi = {10.2174/1567205018666210324121156},
year = {2021},
date = {2021-01-01},
journal = {Current Alzheimer Research},
volume = {18},
number = {1},
pages = {25-34},
publisher = {Bentham Science Publishers},
abstract = {Background: Alzheimer’s disease (AD) is the most common neurodegenerative disorder characterized by cognitive decline and total brain atrophy. Despite the substantial scientific effort, the pathological mechanisms underlying neurodegeneration in AD are currently unknown. In most studies, amyloid β peptide has been considered the key pathological change in AD. However, numerous Aβ-targeting treatments have failed in clinical trials. This implies the need to shift the research focus from Aβ to other pathological features of the disease. Objective: The aim of this study was to examine the interplay between mitochondrial dysfunction, oxidative stress and blood-brain barrier (BBB) disruption in AD pathology, using a novel approach that involves the application of electron paramagnetic resonance (EPR) spectroscopy. Methods: In vivo and ex vivo EPR spectroscopy using two spin probes (aminoxyl radicals) exhibiting different cell-membrane and BBB permeability were employed to assess BBB integrity and brain tissue redox status in the 5xFAD mouse model of AD. In vivo spin probe reduction decay was analyzed using a two-compartment pharmacokinetic model. Furthermore, 15 K EPR spectroscopy was employed to investigate the brain metal content. Results: This study has revealed an altered brain redox state, BBB breakdown, as well as ROS-mediated damage to mitochondrial iron-sulfur clusters, and up-regulation of MnSOD in the 5xFAD model. Conclusion: The EPR spin probes were shown to be excellent in vivo reporters of the 5xFAD neuronal tissue redox state, as well as the BBB integrity, indicating the importance of in vivo EPR spectroscopy application in preclinical studies of neurodegenerative diseases. © 2021 Bentham Science Publishers.},
note = {cited By 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}